cDNA Clone TAK1-S

• Plasmid: pEBG6P-TAK1 1-303 TAB1 371-504 S423A
• Parent Plasmid: pEBG6P
• Expressed: GST-TAK1 1-303 TAB1 371-504 S423A
• Genbank: NM_003188 (TAK1) MAP3K7IP1 (TAB1)
• Tag: GST
• Species: Human

Sequence of Insert

GGATCCATGTCTACAGCCTCTGCCGCCTCCTCCTCCTCCTCGTCTTCGGC
CGGTGAGATGATCGAAGCCCCTTCCCAGGTCCTCAACTTTGAAGAGATCG
ACTACAAGGAGATCGAGGTGGAAGAGGTTGTTGGAAGAGGAGCCTTTGGA
GTTGTTTGCAAAGCTAAGTGGAGAGCAAAAGATGTTGCTATTAAACAAAT
AGAAAGTGAATCTGAGAGGAAAGCGTTTATTGTAGAGCTTCGGCAGTTAT
CCCGTGTGAACCATCCTAATATTGTAAAGCTTTATGGAGCCTGCTTGAAT
CCAGTGTGTCTTGTGATGGAATATGCTGAAGGGGGCTCTTTATATAATGT
GCTGCATGGTGCTGAACCATTGCCATATTATACTGCTGCCCACGCAATGA
GTTGGTGTTTACAGTGTTCCCAAGGAGTGGCTTATCTTCACAGCATGCAA
CCCAAAGCGCTAATTCACAGGGACCTGAAACCACCAAACTTACTGCTGGT
TGCAGGGGGGACAGTTCTAAAAATTTGTGATTTTGGTACAGCCTGTGACA
TTCAGACACACATGACCAATAACAAGGGGAGTGCTGCTTGGATGGCACCT
GAAGTTTTTGAAGGTAGTAATTACAGTGAAAAATGTGACGTCTTCAGCTG
GGGTATTATTCTTTGGGAAGTGATAACGCGTCGGAAACCCTTTGATGAGA
TTGGTGGCCCAGCTTTCCGAATCATGTGGGCTGTTCATAATGGTACTCGA
CCACCACTGATAAAAAATTTACCTAAGCCCATTGAGAGCCTGATGACTCG
TTGTTGGTCTAAAGATCCTTCCCAGCGCCCTTCAATGGAGGAAATTGTGA
AAATAATGACTCACTTGATGCGGTACTTTCCAGGAGCAGATGAGCCATTA
CAGTATCCTTGTCAGGAATTCGGAGGAGGAGGAGGAGAAATGAGCCAGCC
CACACCGAGCCCAGCCCCAGCTGCAGGAGGACGAGTGTACCCTGTGTCTG
TGCCATACTCCAGCGCCCAGAGCACCAGCAAGACCAGCGTGACCCTCTCC
CTTGTCATGCCCTCCCAGGGCCAGATGGTCAACGGGGCTCACGCTGCTTC
CACCCTGGACGAAGCCACCCCCACCCTCACCAACCAAAGCCCGACCTTAA
CCCTGCAGTCCACCAACACGCACACGCAGAGCAGCAGCTCCAGCTCTGAC
GGAGGCCTCTTCCGCTCCCGGCCCGCCCACTCGCTCCCGCCTGGCGAGGA
CGGTCGTGTTGAGCCCTATGTGGACTTTGCTGAGTTTTACCGCCTCTGGA
GCGTGGACCATGGCGAGCAGAGCGTGGTGACAGCACCGTAGGGATCC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLVPRGSLEVLFQGPLGSMSTASAASSSSSS
SAGEMIEAPSQVLNFEEIDYKEIEVEEVVGRGAFGVVCKAKWRAKDVAIK
QIESESERKAFIVELRQLSRVNHPNIVKLYGACLNPVCLVMEYAEGGSLY
NVLHGAEPLPYYTAAHAMSWCLQCSQGVAYLHSMQPKALIHRDLKPPNLL
LVAGGTVLKICDFGTACDIQTHMTNNKGSAAWMAPEVFEGSNYSEKCDVF
SWGIILWEVITRRKPFDEIGGPAFRIMWAVHNGTRPPLIKNLPKPIESLM
TRCWSKDPSQRPSMEEIVKIMTHLMRYFPGADEPLQYPCQEFGGGGGEMS
QPTPSPAPAAGGRVYPVSVPYSSAQSTSKTSVTLSLVMPSQGQMVNGAHA
ASTLDEATPTLTNQSPTLTLQSTNTHTQSSSSSSDGGLFRSRPAHSLPPG
EDGRVEPYVDFAEFYRLWSVDHGEQSVVTAP*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU3499
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N terminal GST for mammalian expression.