cDNA Clone TAPP1

• Plasmid: pGEX-4T TAPP1 PH 182-303
• Parent Plasmid: pGEX4T1
• Expressed: GST-TAPP1 PH 182-303
• Genbank: AF286160
• Tag: GST
• Species: Human

Sequence of Insert

GGATCCATGTTTACTCCTAAACCACCTCAAGATAGTGCGGTTATCAAAGC
TGGATATTGTGTAAAACAAGGAGCAGTGATGAAAAACTGGAAGAGAAGAT
ATTTTCAATTGGATGAAAACACAATAGGCTACTTCAAATCTGAACTGGAA
AAGGAACCTCTTCGCGTAATACCACTTAAAGAGGTTCATAAAGTCCAGGA
ATGTAAGCAAAGCGACATAATGATGAGGGACAACCTCTTTGAAATTGTAA
CAACGTCTCGAACTTTCTATGTGCAGGCTGATAGCCCTGAAGAGATGCAC
AGTTGGATTAAAGCAGTCTCTGGCGCCATTGTAGCACAGCGGGGTCCCGG
CAGATCTGCGTCTTCTGAGCATCCCTGAGGATCC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLVPRGSMFTPKPPQDSAVIKAGYCVKQGAV
MKNWKRRYFQLDENTIGYFKSELEKEPLRVIPLKEVHKVQECKQSDIMMR
DNLFEIVTTSRTFYVQADSPEEMHSWIKAVSGAIVAQRGPGRSASSEHP*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU3463
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N terminal GST for bacterial expression.