cDNA Clone TPS5

• Plasmid: pEBG6P-TPS5
• Parent Plasmid: pEBG6P
• Genbank: CAB10557
• Species: Arabidopsis thaliana

Sequence of Insert

GGATCCATTAGTGATTATCTTCGACATTGTATCTACTTCACTTGCTGT
AGAGATATGGTATCAAGATCTTATTCAAACCTCTTGGATCTTGCTTCTGG
TAACTTCCATTCGTTTTCTCGAGAAAAGAAGAGGTTTCCAAGAGTAGCAA
CTGTCACTGGTGTCTTATCTGAGCTAGATGATGATAACAACAGCAACAGT
GTCTGCTCTGATGCTCCTTCTTCCGTCACACAAGATCGAATCATCATCGT
TGGAAACCAGCTTCCTATTAAATCACATCGGAACTCTGCTGGTAAATTGA
GTTTTAGTTGGGACAATGACTCGCTTCTCTTGCAGCTTAAAGATGGTATG
CGTGAAGATATGGAAGTTGTCTACATTGGTTGCCTTAAGGAACAGATTGA
TACAGTTGAGCAAGATGATGTTTCTCAAAGGTTGCTTGAGAATTTCAAAT
GTGTTCCTGCTTATATCCCACCTGAGCTATTCACTAAGTACTATCATGGA
TTCTGTAAGCAACATCTATGGCCTTTGTTTCACTACATGCTTCCCTTAAC
TCCTGATCTTGGTGGTAGATTTGATCGTTCTTTATGGCAAGCTTATCTTT
CGGTTAACAAGATCTTTGCTGATAAAGTGATGGAAGTGATTAGTCCTGAT
GATGATTTTGTTTGGGTTCATGACTATCACTTAATGGTTTTGCCTACATT
TCTAAGGAAGAGGTTTAATAGAGTAAAGCTTGGTTTTTTCCTTCATAGCC
CGTTCCCTTCCTCTGAGATTTACCGTACTCTTCCAGTGAGAAATGAGCTC
TTACGTGCACTCCTCAACGCTGATTTGATTGGCTTTCATACCTTTGACTA
TGCAAGACACTTCCTTTCTTGTTGTAGCAGGATGCTTGGTTTATCCTATC
AGTCCAAACGTGGAACCATAGGGCTTGAGTATTATGGTCGAACGGTTAGT
ATCAAGATTCTTCCTGTGGGGATTCATATCAGCCAGCTTCAGTCAATTTT
AAACCTCCCAGAGACTCAGACCAAAGTTGCTGAGCTAAGAGATCAGTTCT
TGGATCAGAAAGTTCTTCTCGGTGTTGATGACATGGACATCTTCAAAGGA
ATCAGCCTCAAACTCTTGGCAATGGAACAACTTCTCACACAGCATCCCGA
GAAGAGAGGTCGAGTTGTACTTGTCCAGATTGCAAACCCTGCAAGAGGTC
GTGGGAAAGACGTTCAGGAGGTTCAGTCTGAAACTGAAGCCACGGTTAAA
AGGATCAATGAAATGTTTGGAAGGCCGGGCTACCAACCCGTGGTTCTGAT
TGATACACCGCTTCAATTCTTTGAGAGGATTGCTTACTATGTCATTGCAG
AGTGTTGTCTTGTTACAGCGGTAAGAGATGGTATGAATCTTATACCTTAT
GAGTACATTATCTGCAGGCAAGGTAATCCGAAACTCAACGAGACTATAGG
CCTTGACCCTTCTGCTGCAAAGAAGAGTATGCTTGTTGTCTCTGAGTTTA
TTGGTTGTTCTCCTTCTTTAAGCGGCGCCATTAGAGTAAATCCGTGGAAC
ATTGATGCTGTGACTGAAGCAATGGACTATGCATTGATAGTTTCAGAAGC
AGAGAAGCAAATGCGTCACGAGAAGCATCACAAATATGTTAGCACACATG
ATGTTGCTTATTGGGCGCGTAGCTTTATACAAGATCTTGAAAGGGCTTGC
GGGGATCATGTGAGGAAGAGGTGTTGGGGGATTGGATTCGGGTTAGGCTT
TAGAGTTGTGGCGCTTGATCCGAGTTTTAAAAAGCTTTCGATTGAGCACA
TTGTCTCAGCTTATAAGAGAACCAAGAACCGAGCCATTTTGTTGGATTAT
GATGGCACAATGGTGCAGCCAGGTTCCATTAGGACAACACCAACCCGCGA
AACAATCGAAATCTTGAACAACCTGTCTAGTGATCCCAAGAATATCGTGT
ACCTCGTCAGTGGGAAAGACAGAAGGACACTAACTGAATGGTTTTCTTCA
TGTGACGATCTCGGTTTGGGTGCAGAGCACGGGTATTTTATAAGGCCAAA
TGATGGAACAGACTGGGAAACGTCGAGTTTGGTATCAGGTTTTGAGTGGA
AACAAATAGCAGAGCCAGTGATGAGACTTTACACTGAGACTACAGATGGA
TCAACAATAGAGACTAAAGAGACTGCTCTCGTTTGGAATTACCAATTCGC
AGATCCTGATTTTGGATCTTGTCAAGCCAAAGAGCTTATGGAACACCTCG
AAAGCGTGCTTACCAATGATCCAGTCTCTGTCAAGACTGGACAACAACTC
GTTGAAGTTAAACCACAGGGTGTGAACAAAGGTCTTGTGGCAGAGAGGCT
TCTAACAACGATGCAAGAAAAAGGGAAACTTTTGGATTTCATTCTCTGCG
TCGGTGATGATCGGTCTGATGAGGATATGTTTGAGGTGATAATGAGTGCT
AAAGATGGTCCAGCTTTGTCTCCTGTGGCTGAGATATTCGCTTGCACCGT
TGGACAAAAGCCAAGCAAAGCAAAATACTATTTAGACGATACTGCGGAGA
TAATCAGAATGCTGGACGGTCTAGCCGCTACCAACACAACTATCTCTGAT
CAAACTGATTCAACCGCCACTGTTCCAACTAAAGATCTGTTTTAAGAATT
CGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLVPRGSLEVLFQGPLGSISDYLRHCIYFTC
CRDMVSRSYSNLLDLASGNFHSFSREKKRFPRVATVTGVLSELDDDNNSN
SVCSDAPSSVTQDRIIIVGNQLPIKSHRNSAGKLSFSWDNDSLLLQLKDG
MREDMEVVYIGCLKEQIDTVEQDDVSQRLLENFKCVPAYIPPELFTKYYH
GFCKQHLWPLFHYMLPLTPDLGGRFDRSLWQAYLSVNKIFADKVMEVISP
DDDFVWVHDYHLMVLPTFLRKRFNRVKLGFFLHSPFPFPEIYRTLPVRNE
LLRALLNADLIGFHTFDYARHFLSCCSRMLGLSYQSKRGTIGLEYYGRTG
NIKILPVGIHISQLQSILNLPETQTKVAELRDQFLDQKVLLGVDDMDIFK
GISLKLLAMEQLLTQHPEKRGRVVLVQIANPARGRGKDVQEVQSETEATV
KRINEMFGRPGYQPVVLIDTPLQFFERIAYYVIAECCLVTAVRDGMNLIP
YEYIICRQGNPKLNETIGLDPSAAKKSMLVVSEFIGCSPSLSGAIRVNPW
NIDAVTEAMDYALIVSEAEKQMRHEKHHKYVSTHDVAYWARSFIQDLERA
CGDHVRKRCWGIGFGLGFRVVALDPSFKKLSIEHIVSAYKRTKNRAILLD
YDGTMVQPGSIRTTPTRETIEILNNLSSDPKNIVYLVSGKDRRTLTEWFS
SCDDLGLGAEHGYFIRPNDGTDWETSSLVSGFEWKQIAEPVMRLYTETTD
GSTIETKETALVWNYQFADPDFGSCQAKELMEHLESVLTNDPVSVKTGQQ
LVEVKPQGVNKGLVAERLLTTMQEKGKLLDFILCVGDDRSDEDMFEVIMS
AKDGPALSPVAEIFACTVGQKPSKAKYYLDDTAEIIRMLDGLAATNTTIS
DQTDSTATVPTKDLF*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU3454
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Mammalian expression plasmid with N terminal GST tag.
• Low Temp: No