cDNA Clone DYRK3

• Plasmid: pGEX6P-1-DYRK3
• Parent Plasmid: pGEX6P1
• Expressed: GST-DYRK3
• Genbank: AY590695
• Tag: GST
• Species: Human

Sequence of Insert

GGATCCATGGGAGGCACAGCTCGTGGGCCTGGGCGGAAGGATGCGGGGCC
GCCTGGGGCCGGGCTCCCGCCCCAGCAGCGGAGGTTGGGGGATGGTGTCT
ATGACACCTTCATGATGATAGATGAAACCAAATGTCCCCCCTGTTCAAAT
GTACTCTGCAATCCTTCTGAACCACCTCCACCCAGAAGACTAAATATGAC
CACTGAGCAGTTTACAGGAGATCATACTCAGCACTTTTTGGATGGAGGTG
AGATGAAGGTAGAACAGCTGTTTCAAGAATTTGGCAACAGAAAATCCAAT
ACTATTCAGTCAGATGGCATCAGTGACTCTGAAAAATGCTCTCCTACTGT
TTCTCAGGGTAAAAGTTCAGATTGCTTGAATACAGTAAAATCCAACAGTT
CATCCAAGGCACCCAAAGTGGTGCCTCTGACTCCAGAACAAGCCCTGAAG
CAATATAAACACCACCTCACTGCCTATGAGAAACTGGAAATAATTAATTA
TCCAGAAATTTACTTTGTAGGTCCAAATGCCAAGAAAAGACATGGAGTTA
TTGGTGGTCCCAATAATGGAGGGTATGATGATGCAGATGGGGCCTATATT
CATGTACCTCGAGACCATCTAGCTTATCGATATGAGGTGCTGAAAATTAT
TGGCAAGGGGAGTTTTGGGCAGGTGGCCAGGGTCTATGATCACAAACTTC
GACAGTACGTGGCCCTAAAAATGGTGCGCAATGAGAAGCGCTTTCATCGT
CAAGCAGCTGAGGAGATCCGGATTTTGGAGCATCTTAAGAAACAGGATAA
AACTGGTAGTATGAACGTTATCCACATGCTGGAAAGTTTCACATTCCGGA
ACCATGTTTGCATGGCCTTTGAATTGCTGAGCATAGACCTTTATGAGCTG
ATTAAAAAAAATAAGTTTCAGGGTTTTAGCGTCCAGTTGGTACGCAAGTT
TGCCCAGTCCATCTTGCAATCTTTGGATGCCCTCCACAAAAATAAGATTA
TTCACTGCGATCTGAAGCCAGAAAACATTCTCCTGAAACACCACGGGCGC
AGTTCAACCAAGGTCATTGACTTTGGGTCCAGCTGTTTCGAGTACCAGAA
GCTCTACACATATATCCAGTCTCGGTTCTACAGAGCTCCAGAAATCATCT
TAGGAAGCCGCTACAGCACACCAATTGACATATGGAGTTTTGGCTGCATC
CTTGCAGAACTTTTAACAGGACAGCCTCTCTTCCCTGGAGAGGATGAAGG
AGACCAGTTGGCCTGCATGATGGAGCTTCTAGGGATGCCACCACCAAAAC
TTCTGGAGCAATCCAAACGTGCCAAGTACTTTATTAATTCCAAGGGCATA
CCCCGCTACTGCTCTGTGACTACCCAGGCAGATGGGAGGGTTGTGCTTGT
GGGGGGTCGCTCACGTAGGGGTAAAAAGCGGGGTCCCCCAGGCAGCAAAG
ACTGGGGGACAGCACTGAAAGGGTGTGATGACTACTTGTTTATAGAGTTC
TTGAAAAGGTGTCTTCACTGGGACCCCTCTGCCCGCTTGACCCCAGCTCA
AGCATTAAGACACCCTTGGATTAGCAAGTCTGTCCCCAGACCTCTCACCA
CCATAGACAAGGTGTCAGGGAAACGGGTAGTTAATCCTGCAAGTGCTTTC
CAGGGATTGGGTTCTAAGCTGCCTCCAGTTGTTGGAATAGCCAATAAGCT
TAAAGCTAACTTAATGTCAGAAACCAATGGTAGTATACCCCTATGCAGTG
TATTGCCAAAACTGATTAGCTAGGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMGGTARGPGRKDAGPPGAG
LPPQQRRLGDGVYDTFMMIDETKCPPCSNVLCNPSEPPPPRRLNMTTEQF
TGDHTQHFLDGGEMKVEQLFQEFGNRKSNTIQSDGISDSEKCSPTVSQGK
SSDCLNTVKSNSSSKAPKVVPLTPEQALKQYKHHLTAYEKLEIINYPEIY
FVGPNAKKRHGVIGGPNNGGYDDADGAYIHVPRDHLAYRYEVLKIIGKGS
FGQVARVYDHKLRQYVALKMVRNEKRFHRQAAEEIRILEHLKKQDKTGSM
NVIHMLESFTFRNHVCMAFELLSIDLYELIKKNKFQGFSVQLVRKFAQSI
LQSLDALHKNKIIHCDLKPENILLKHHGRSSTKVIDFGSSCFEYQKLYTY
IQSRFYRAPEIILGSRYSTPIDIWSFGCILAELLTGQPLFPGEDEGDQLA
CMMELLGMPPPKLLEQSKRAKYFINSKGIPRYCSVTTQADGRVVLVGGRS
RRGKKRGPPGSKDWGTALKGCDDYLFIEFLKRCLHWDPSARLTPAQALRH
PWISKSVPRPLTTIDKVSGKRVVNPASAFQGLGSKLPPVVGIANKLKANL
MSETNGSIPLCSVLPKLIS*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU3371
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N terminal GST for bacterial expression.