cDNA Clone TPS5

• Synonyms: ATTPS5; DL4920W; FCAALL.9; TREHALOSE -6-PHOSPHATASE SYNTHASE S5; trehalose phosphatase/synthase 5; AT4G17770
• Plasmid: pET28A Arabidopsis TPS5
• Parent Plasmid: pET28a original
• Expressed: 6His Thrombin T7 Arabidopsis TPS5
• Genbank: CAB10557.1
• Tag: 6His
• Tag: T7
• Species: Arabidopsis thaliana

Sequence of Insert

GGATCCATTAGTGATTATCTTCGACATTGTATCTACTTCACTTGCTGTAGAGATATGGTATCAAGATCTTATTCAAACCTCTTGGATCTTGCTTCTGGTAACTTCCATTCGTTTTCTCGAGAAAAGAAGAGGTTTCCAAGAGTAGCAACTGTCACTGGTGTCTTATCTGAGCTAGATGATGATAACAACAGCAACAGTGTCTGCTCTGATGCTCCTTCTTCCGTCACACAAGATCGAATCATCATCGTTGGAAACCAGCTTCCTATTAAATCACATCGGAACTCTGCTGGTAAATTGAGTTTTAGTTGGGACAATGACTCGCTTCTCTTGCAGCTTAAAGATGGTATGCGTGAAGATATGGAAGTTGTCTACATTGGTTGCCTTAAGGAACAGATTGATACAGTTGAGCAAGATGATGTTTCTCAAAGGTTGCTTGAGAATTTCAAATGTGTTCCTGCTTATATCCCACCTGAGCTATTCACTAAGTACTATCATGGATTCTGTAAGCAACATCTATGGCCTTTGTTTCACTACATGCTTCCCTTAACTCCTGATCTTGGTGGTAGATTTGATCGTTCTTTATGGCAAGCTTATCTTTCGGTTAACAAGATCTTTGCTGATAAAGTGATGGAAGTGATTAGTCCTGATGATGATTTTGTTTGGGTTCATGACTATCACTTAATGGTTTTGCCTACATTTCTAAGGAAGAGGTTTAATAGAGTAAAGCTTGGTTTTTTCCTTCATAGCCCGTTCCCTTCCTCTGAGATTTACCGTACTCTTCCAGTGAGAAATGAGCTCTTACGTGCACTCCTCAACGCTGATTTGATTGGCTTTCATACCTTTGACTATGCAAGACACTTCCTTTCTTGTTGTAGCAGGATGCTTGGTTTATCCTATCAGTCCAAACGTGGAACCATAGGGCTTGAGTATTATGGTCGAACGGTTAGTATCAAGATTCTTCCTGTGGGGATTCATATCAGCCAGCTTCAGTCAATTTTAAACCTCCCAGAGACTCAGACCAAAGTTGCTGAGCTAAGAGATCAGTTCTTGGATCAGAAAGTTCTTCTCGGTGTTGATGACATGGACATCTTCAAAGGAATCAGCCTCAAACTCTTGGCAATGGAACAACTTCTCACACAGCATCCCGAGAAGAGAGGTCGAGTTGTACTTGTCCAGATTGCAAACCCTGCAAGAGGTCGTGGGAAAGACGTTCAGGAGGTTCAGTCTGAAACTGAAGCCACGGTTAAAAGGATCAATGAAATGTTTGGAAGGCCGGGCTACCAACCCGTGGTTCTGATTGATACACCGCTTCAATTCTTTGAGAGGATTGCTTACTATGTCATTGCAGAGTGTTGTCTTGTTACAGCGGTAAGAGATGGTATGAATCTTATACCTTATGAGTACATTATCTGCAGGCAAGGTAATCCGAAACTCAACGAGACTATAGGCCTTGACCCTTCTGCTGCAAAGAAGAGTATGCTTGTTGTCTCTGAGTTTATTGGTTGTTCTCCTTCTTTAAGCGGCGCCATTAGAGTAAATCCGTGGAACATTGATGCTGTGACTGAAGCAATGGACTATGCATTGATAGTTTCAGAAGCAGAGAAGCAAATGCGTCACGAGAAGCATCACAAATATGTTAGCACACATGATGTTGCTTATTGGGCGCGTAGCTTTATACAAGATCTTGAAAGGGCTTGCGGGGATCATGTGAGGAAGAGGTGTTGGGGGATTGGATTCGGGTTAGGCTTTAGAGTTGTGGCGCTTGATCCGAGTTTTAAAAAGCTTTCGATTGAGCACATTGTCTCAGCTTATAAGAGAACCAAGAACCGAGCCATTTTGTTGGATTATGATGGCACAATGGTGCAGCCAGGTTCCATTAGGACAACACCAACCCGCGAAACAATCGAAATCTTGAACAACCTGTCTAGTGATCCCAAGAATATCGTGTACCTCGTCAGTGGGAAAGACAGAAGGACACTAACTGAATGGTTTTCTTCATGTGACGATCTCGGTTTGGGTGCAGAGCACGGGTATTTTATAAGGCCAAATGATGGAACAGACTGGGAAACGTCGAGTTTGGTATCAGGTTTTGAGTGGAAACAAATAGCAGAGCCAGTGATGAGACTTTACACTGAGACTACAGATGGATCAACAATAGAGACTAAAGAGACTGCTCTCGTTTGGAATTACCAATTCGCAGATCCTGATTTTGGATCTTGTCAAGCCAAAGAGCTTATGGAACACCTCGAAAGCGTGCTTACCAATGATCCAGTCTCTGTCAAGACTGGACAACAACTCGTTGAAGTTAAACCACAGGGTGTGAACAAAGGTCTTGTGGCAGAGAGGCTTCTAACAACGATGCAAGAAAAAGGGAAACTTTTGGATTTCATTCTCTGCGTCGGTGATGATCGGTCTGATGAGGATATGTTTGAGGTGATAATGAGTGCTAAAGATGGTCCAGCTTTGTCTCCTGTGGCTGAGATATTCGCTTGCACCGTTGGACAAAAGCCAAGCAAAGCAAAATACTATTTAGACGATACTGCGGAGATAATCAGAATGCTGGACGGTCTAGCCGCTACCAACACAACTATCTCTGATCAAACTGATTCAACCGCCACTGTTCCAACTAAAGATCTGTTTTAAGAATTC

Amino Acid Sequence

MGSSHHHHHHSSGLVPRGSHMASMTGGQQMGRGSISDYLRHCIYFTCCRDMVSRSYSNLLDLASGNFHSFSREKKRFPRVATVTGVLSELDDDNNSNSVC
SDAPSSVTQDRIIIVGNQLPIKSHRNSAGKLSFSWDNDSLLLQLKDGMREDMEVVYIGCLKEQIDTVEQDDVSQRLLENFKCVPAYIPPELFTKYYHGFC
KQHLWPLFHYMLPLTPDLGGRFDRSLWQAYLSVNKIFADKVMEVISPDDDFVWVHDYHLMVLPTFLRKRFNRVKLGFFLHSPFPSSEIYRTLPVRNELLR
ALLNADLIGFHTFDYARHFLSCCSRMLGLSYQSKRGTIGLEYYGRTVSIKILPVGIHISQLQSILNLPETQTKVAELRDQFLDQKVLLGVDDMDIFKGIS
LKLLAMEQLLTQHPEKRGRVVLVQIANPARGRGKDVQEVQSETEATVKRINEMFGRPGYQPVVLIDTPLQFFERIAYYVIAECCLVTAVRDGMNLIPYEY
IICRQGNPKLNETIGLDPSAAKKSMLVVSEFIGCSPSLSGAIRVNPWNIDAVTEAMDYALIVSEAEKQMRHEKHHKYVSTHDVAYWARSFIQDLERACGD
HVRKRCWGIGFGLGFRVVALDPSFKKLSIEHIVSAYKRTKNRAILLDYDGTMVQPGSIRTTPTRETIEILNNLSSDPKNIVYLVSGKDRRTLTEWFSSCD
DLGLGAEHGYFIRPNDGTDWETSSLVSGFEWKQIAEPVMRLYTETTDGSTIETKETALVWNYQFADPDFGSCQAKELMEHLESVLTNDPVSVKTGQQLVE
VKPQGVNKGLVAERLLTTMQEKGKLLDFILCVGDDRSDEDMFEVIMSAKDGPALSPVAEIFACTVGQKPSKAKYYLDDTAEIIRMLDGLAATNTTISDQT
DSTATVPTKDLF*

• Vector Type: Bacterial
• Antibiotic: Kan
• Full Snapgene Map: pET28A Arabidopsis TPS566.42 KB Snapgene files can be opened using software available here: www.snapgene.com
• DU Number: DU3352
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee