cDNA Clone ERK8

• Synonyms: MAPK15
• Plasmid: pCMV5-HA ERK8
• Parent Plasmid: pCMV5
• Expressed: HA-ERK8
• Genbank: AY065978
• Tag: HA
• Species: Human

Sequence of Insert

GGATCCGCCACCATGTACCCATACGATGTGCCAGATTACGCCTGCACCGT
AGTGGACCCTCGCATTGTCCGGAGATACCTACTCAGGCGGCAGCTCGGGC
AGGGGGCCTATGGCATTGTGTGGAAGGCAGTGGACCGGAGGACTGGTGAG
GTCGTGGCCATCAAGAAAATCTTTGATGCTTTTAGGGATAAGACAGATGC
CCAGAGAACATTCCGGGAAATCACGCTCCTCCAGGAGTTTGGGGACCATC
CCAACATCATCAGCCTCCTTGACGTGATCCGGGCAGAGAACGACAGGGAC
ATTTACCTGGTGTTTGAGTTTATGGACACTGACCTGAACGCAGTCATCCG
GAAGGGCGGCCTGCTGCAGGACGTCCACGTGCGCTCCATCTTCTACCAGC
TCCTGCGGGCCACCCGGTTCCTCCACTCGGGGCACGTTGTGCACCGGGAC
CAGAAGCCGTCCAATGTGCTCCTGGATGCCAACTGCACAGTGAAGCTGTG
TGACTTTGGCCTGGCCCGCTCCCTGGGCGACCTCCCTGAGGGGCCTGAGG
ACCAGGCCGTGACAGAGTACGTGGCCACACGCTGGTACCGAGCACCGGAG
GTGCTGCTCTCTTCGCACCGATACACCCTTGGGGTGGACATGTGGAGTCT
GGGCTGTATCCTGGGGGAGATGCTGCGGGGGAGACCCCTGTTCCCCGGCA
CGTCCACCCTCCACCAGCTGGAGCTGATCCTGGAGACCATCCCACCGCCA
TCTGAGGAGGACCTCCTGGCTCTCGGCTCAGGCTGCCGTGCCTCTGTGCT
GCACCAGCTGGGGTCCCGGCCACGACAGACGCTGGATGCCCTCCTACCGC
CAGACACCTCCCCAGAGGCCTTGGACCTCCTTAGGCGACTCCTGGTGTTC
GCCCCGGACAAGCGGTTAAGCGCGACCCAGGCACTGCAGCACCCCTACGT
GCAGAGGTTCCACTGCCCCAGCGACGAGTGGGCACGAGAGGCAGATGTGC
GGCCCCGGGCACACGAAGGGGTCCAGCTCTCTGTGCCTGAGTACCGCAGC
CGCGTCTATCAGATGATCCTGGAGTGTGGAGGCAGCAGCGGCACCTCGAG
AGAGAAGGGCCCGGAGGGTGTCTCCCCAAGCCAGGCACACCTGCACAAAC
CCAGAGCCGACCCTCAGCTGCCTTCTAGGACACCTGTGCAGGGTCCCAGA
CCCAGGCCCCAGAGCAGCCCAGGCCATGACCCTGCCGAGCACGAGTCCCC
CCGTGCAGCCAAGAACGTTCCCAGGCAGAACTCCGCTCCCCTGCTCCAAA
CTGCTCTCCTAGGGAATGGGGAAGGGCCCCCTGGGGCGAAGGAAGCGCCC
CCCTTGACACTCTCGCTGGTGAAGCCAGGCGGGAGGGGAGCTGCGCCCTC
CCTGACCTCCCAGGCTGCGGCTCAGGTGGCCAACCAGGCCCTGATCCGGG
GTGACTGGAACCGGGGCGGTGGGGTGAGGGTGGCCAGCGTACAACAGGTC
CCTCCCCGGCTTCCTCCGGAGGCCCGGCCCGGCCGGAGGATGTTCAGCAC
CTCTGCCTTGCAGGGTGCCCAGGGGGGTGCCAGGGCTTTGCTTGGAGGCT
ACTCCCAAGCCTACGGGACTGTCTGCCACTCGGCACTGGGCCACCTGCCC
CTGCTGGAGGGGCACCATGTGTGAGAATTCGCAAGGGCGAATTCCAGCAC
ACTGGCGGCCGTTACTAGGTGGATCC

Amino Acid Sequence

MYPYDVPDYACTVVDPRIVRRYLLRRQLGQGAYGIVWKAVDRRTGEVVAI
KKIFDAFRDKTDAQRTFREITLLQEFGDHPNIISLLDVIRAENDRDIYLV
FEFMDTDLNAVIRKGGLLQDVHVRSIFYQLLRATRFLHSGHVVHRDQKPS
NVLLDANCTVKLCDFGLARSLGDLPEGPEDQAVTEYVATRWYRAPEVLLS
SHRYTLGVDMWSLGCILGEMLRGRPLFPGTSTLHQLELILETIPPPSEED
LLALGSGCRASVLHQLGSRPRQTLDALLPPDTSPEALDLLRRLLVFAPDK
RLSATQALQHPYVQRFHCPSDEWAREADVRPRAHEGVQLSVPEYRSRVYQ
MILECGGSSGTSREKGPEGVSPSQAHLHKPRADPQLPSRTPVQGPRPRPQ
SSPGHDPAEHESPRAAKNVPRQNSAPLLQTALLGNGEGPPGAKEAPPLTL
SLVKPGGRGAAPSLTSQAAAQVANQALIRGDWNRGGGVRVASVQQVPPRL
PPEARPGRRMFSTSALQGAQGGARALLGGYSQAYGTVCHSALGHLPLLEG
HHV*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU3326
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Mammalian expression plasmid with N terminal HA tag.