cDNA Clone DAZAP1-b

• Plasmid: pGEX6P-1-DAZAP1-b T269A T315A
• Parent Plasmid: pGEX6P1
• Expressed: GST-DAZAP1-b T269A T315A
• Genbank: NM_018959
• Tag: GST
• Species: Human

Sequence of Insert

GGATCCAACAACTCGGGCGCCGACGAGATCGGGAAGCTCTTCGTGGGCGG
TCTTGACTGGAGCACGACCCAAGAGACTCTGCGCAGCTACTTTTCCCAAT
ATGGAGAAGTCGTAGATTGTGTTATCATGAAAGATAAAACCACCAACCAG
TCTCGAGGCTTTGGGTTTGTCAAATTTAAAGACCCAAACTGTGTGGGGAC
GGTGCTGGCCAGCAGACCGCACACGCTAGATGGCCGAAACATCGACCCCA
AGCCATGCACACCCCGGGGGATGCAGCCGGAGAGAACACGGCCGAAGGAA
GGATGGCAGAAAGGACCCAGGAGCGATAACAGTAAATCAAATAAGATATT
TGTCGGTGGAATTCCTCACAATTGTGGTGAGACAGAGCTCAGGGAATACT
TCAAGAAGTTCGGAGTGGTCACGGAGGTAGTCATGATCTATGACGCCGAG
AAGCAGAGGCCCCGAGGTTTTGGATTTATTACTTTCGAGGACGAACAATC
AGTGGACCAGGCTGTCAACATGCATTTTCACGACATCATGGGCAAAAAAG
TGGAAGTTAAACGAGCTGAGCCTCGGGACAGCAAGAGCCAAGCGCCGGGA
CAGCCAGGTGCCAGCCAGTGGGGGAGCCGGGTTGTGCCCAACGCTGCCAA
TGGCTGGGCAGGCCAGCCCCCGCCCACGTGGCAGCAAGGATATGGCCCGC
AAGGAATGTGGGTGCCGGCAGGACAGGCGATTGGTGGCTATGGACCGCCC
CCTGCAGGAAGAGGAGCCCCCCCGCCACCCCCACCGTTCACCTCCTACAT
CGTGTCCGCCCCTCCTGGAGGCTTTCCCCCTCCCCAGGGCTTCCCTCAGG
GCTACGGTGCCCCGCCACAGTTCAGTTTTGGCTACGGGCCTCCACCTCCA
CCGCCAGATCAGTTTGCCCCTCCGGGGGTTCCTCCTCCACCAGCCGCTCC
CGGGGCAGCACCTCTGGCTTTCCCACCGCCTCCGTCTCAGGCTGCCCCGG
ACATGAGCAAGCCCCCGACAGCTCAGCCAGACTTCCCCTATGGTCAGTAT
GCAGGTTACGGGCAGGACTTGAGTGGCTTCGGACAGGGCTTCTCAGACCC
CAGCCAGCAGCCTCCTTCCTACGGGGGTCCCTCCGTGCCAGGGTCGGGGG
GCCCCCCCGCCGGCGGCAGCGGCTTTGGACGAGGGCAGAACCACAACGTG
CAAGGGTTCCACCCCTACCGACGCTAGGGATCC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSNNSGADEIGKLFVGGLDWS
TTQETLRSYFSQYGEVVDCVIMKDKTTNQSRGFGFVKFKDPNCVGTVLAS
RPHTLDGRNIDPKPCTPRGMQPERTRPKEGWQKGPRSDNSKSNKIFVGGI
PHNCGETELREYFKKFGVVTEVVMIYDAEKQRPRGFGFITFEDEQSVDQA
VNMHFHDIMGKKVEVKRAEPRDSKSQAPGQPGASQWGSRVVPNAANGWAG
QPPPTWQQGYGPQGMWVPAGQAIGGYGPPPAGRGAPPPPPPFTSYIVSAP
PGGFPPPQGFPQGYGAPPQFSFGYGPPPPPPDQFAPPGVPPPPAAPGAAP
LAFPPPPSQAAPDMSKPPTAQPDFPYGQYAGYGQDLSGFGQGFSDPSQQP
PSYGGPSVPGSGGPPAGGSGFGRGQNHNVQGFHPYRR*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU3267
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N terminal GST tag for bacterial expression.