cDNA Clone MEKK1

• Synonyms: MAP3K1
• Plasmid: pGFP TAPP new MEKK1 D1-622 human
• Parent Plasmid: pGFP TAP BN
• Expressed: GFP-TAP-new MEKK1 D1-622 human
• Genbank: AF042838
• Tag: GFP
• Tag: TAP
• Species: Human

Sequence of Insert

GGATCCATGGTTACTACAGTACCCCATGTGTTTTCAAAACTGTTAGAAAT
GCTGAGTGTTTCCAGTTCCACTCACTTCACCAGGATGCGTCGCCGTTTGA
TGGCTATTGCAGATGAGGTGGAAATTGCCGAAGCCATCCAGTTGGGCGTA
GAAGACACTTTGGATGGTCAACAGGACAGCTTCTTGCAGGCATCTGTTCC
CAACAACTATCTGGAAACCACAGAGAACAGTTCCCCTGAGTGCACAATCC
ATTTAGAGAAAACTGGAAAAGGATTATGTGCTACAAAATTGAGTGCCAGT
TCAGAGGACATTTCTGAGAGACTGGCCAGCATTTCAGTAGGACCTTCTAG
TTCAACAACAACAACAACAACAACAGAGCAACCAAAGCCAATGGTTCAAA
CAAAAGGCAGACCCCACAGTCAGTGTTTGAACTCCTCTCCTTTATCTCAT
CATTCCCAATTAATGTTTCCAGCCTTGTCAACCCCTTCTTCTTCTACCCC
ATCTGTACCAGCTGGCACTGCAACAGATGTCTCTAAGCATAGACTTCAGG
GATTCATTCCCTGCAGAATACCTTCTGCATCTCCTCAAACACAGCGCAAG
TTTTCTCTACAATTCCACAGAAACTGTCCTGAAAACAAAGACTCAGATAA
ACTTTCCCCAGTCTTTACTCAGTCAAGACCCTTGCCCTCCAGTAACATAC
ACAGGCCAAAGCCATCTCGACCTACCCCAGGTAATACAAGTAAACAGGGA
GATCCCTCAAAAAATAGCATGACACTTGATCTGAACAGTAGTTCCAAATG
TGATGACAGCTTTGGCTGTAGCAGCAATAGTAGTAATGCTGTTATACCCA
GTGACGAGACAGTGTTCACCCCAGTAGAGGAGAAATGCAGATTAGATGTC
AATACAGAGCTCAACTCCAGTATTGAGGACCTTCTTGAAGCATCTATGCC
TTCAAGTGATACAACAGTAACTTTTAAGTCAGAAGTTGCTGTCCTGTCTC
CTGAAAAGGCTGAAAATGATGATACCTACAAAGATGATGTGAATCATAAT
CAAAAGTGCAAAGAGAAGATGGAAGCTGAAGAAGAAGAAGCTTTAGCAAT
TGCCATGGCAATGTCAGCGTCTCAGGATGCCCTCCCCATAGTTCCTCAGC
TGCAGGTTGAAAATGGAGAAGATATCATCATTATTCAACAGGATACACCA
GAGACTCTACCAGGACATACCAAAGCAAAACAACCGTATAGAGAAGACAC
TGAATGGCTGAAAGGTCAACAGATAGGCCTTGGAGCATTTTCTTCTTGTT
ATCAGGCTCAAGATGTGGGAACTGGAACTTTAATGGCTGTTAAACAGGTG
ACTTATGTCAGAAACACATCTTCTGAGCAAGAAGAAGTAGTAGAAGCACT
AAGAGAAGAGATAAGAATGATGAGCCATCTGAATCATCCAAACATCATTA
GGATGTTGGGAGCCACGTGTGAGAAGAGCAATTACAATCTCTTCATTGAA
TGGATGGCAGGGGGATCGGTGGCTCATTTGCTGAGTAAATATGGAGCCTT
CAAAGAATCAGTAGTTATTAACTACACTGAACAGTTACTCCGTGGCCTTT
CGTATCTCCATGAAAACCAAATCATTCACAGAGATGTCAAAGGTGCCAAT
TTGCTAATTGACAGCACTGGTCAGAGACTAAGAATTGCAGATTTTGGAGC
TGCAGCCAGGTTGGCATCAAAAGGAACTGGTGCAGGAGAGTTTCAGGGAC
AATTACTGGGGACAATTGCATTTATGGCACCTGAGGTACTAAGAGGTCAA
CAGTATGGAAGGAGCTGTGATGTATGGAGTGTTGGCTGTGCTATTATAGA
AATGGCTTGTGCAAAACCACCATGGAATGCAGAAAAACACTCCAATCATC
TTGCTTTGATATTTAAGATTGCTAGTGCAACTACTGCTCCATCGATCCCT
TCACATTTGTCTCCTGGTTTACGAGATGTGGCTCTTCGTTGTTTAGAACT
TCAACCTCAGGACAGACCTCCATCAAGAGAGCTACTGAAGCATCCAGTCT
TTCGTACTACATGGCATCATCACCATCACCATTGAGAATTCGC

Amino Acid Sequence

MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICT
TGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIF
FKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHN
VYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
YLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKSGRTQISSSSF
DIALATMDTSAHDEAVDNKFNKEQQNAFYEILHLPNLNEEQRNAFIQSLK
DDPSQSANLLAEAKKLNDAQAPKVDNKFNKERQNAFYEILHLPNLNEEQR
NAFIQSLKDDPSQSANLLAEAKKLNDAQAPKVDASWSAGASENLYFQGGR
SMEKRRWKKNFIAVSAANRFKKISSSGALDYDIPTALEVLFQGPLGSMVT
TVPHVFSKLLEMLSVSSSTHFTRMRRRLMAIADEVEIAEAIQLGVEDTLD
GQQDSFLQASVPNNYLETTENSSPECTIHLEKTGKGLCATKLSASSEDIS
ERLASISVGPSSSTTTTTTTEQPKPMVQTKGRPHSQCLNSSPLSHHSQLM
FPALSTPSSSTPSVPAGTATDVSKHRLQGFIPCRIPSASPQTQRKFSLQF
HRNCPENKDSDKLSPVFTQSRPLPSSNIHRPKPSRPTPGNTSKQGDPSKN
SMTLDLNSSSKCDDSFGCSSNSSNAVIPSDETVFTPVEEKCRLDVNTELN
SSIEDLLEASMPSSDTTVTFKSEVAVLSPEKAENDDTYKDDVNHNQKCKE
KMEAEEEEALAIAMAMSASQDALPIVPQLQVENGEDIIIIQQDTPETLPG
HTKAKQPYREDTEWLKGQQIGLGAFSSCYQAQDVGTGTLMAVKQVTYVRN
TSSEQEEVVEALREEIRMMSHLNHPNIIRMLGATCEKSNYNLFIEWMAGG
SVAHLLSKYGAFKESVVINYTEQLLRGLSYLHENQIIHRDVKGANLLIDS
TGQRLRIADFGAAARLASKGTGAGEFQGQLLGTIAFMAPEVLRGQQYGRS
CDVWSVGCAIIEMACAKPPWNAEKHSNHLALIFKIASATTAPSIPSHLSP
GLRDVALRCLELQPQDRPPSRELLKHPVFRTTWHHHHHH*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU3256
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N terminal GFP TAPP/ C terminal 6His for mammalian expression n.b. numbering in name relates to Celera protein hCP1814173. M1 of hCP1814173 is M145 in AF042838