cDNA Clone NDRG1

• Plasmid: pEBG-2T-FLAG NDRG1 T346A
• Parent Plasmid: pEBG2T
• Expressed: GST-FLAG NDRG1 T346A
• Genbank: XP_005243
• Tag: GST
• Tag: FLAG
• Species: Human

Sequence of Insert

GGATCCGCCACCATGGACTACAAGGACGACGATGACAAGTCTCGGGAGAT
GCAGGATGTAGACCTCGCTGAGGTGAAGCCTTTGGTGGAGAAAGGGGAGA
CCATCACCGGCCTCCTGCAAGAGTTTGATGTCCAGGAGCAGGACATCGAG
ACTTTACATGGCTCTGTTCACGTCACGCTGTGTGGGACTCCCAAGGGAAA
CCGGCCTGTCATCCTCACCTACCATGACATCGGCATGAACCACAAAACCT
GCTACAACCCCCTCTTCAACTACGAGGACATGCAGGAGATCACCCAGCAC
TTTGCCGTCTGCCACGTGGACGCCCCTGGCCAGCAGGACGGCGCAGCCTC
CTTCCCCGCAGGGTACATGTACCCCTCCATGGATCAGCTGGCTGAAATGC
TTCCTGGAGTCCTTCAACAGTTTGGGCTGAAAAGCATTATTGGCATGGGA
ACAGGAGCAGGCGCCTACATCCTAACTCGATTTGCTCTAAACAACCCTGA
GATGGTGGAGGGCCTTGTCCTTATCAACGTGAACCCTTGTGCGGAAGGCT
GGATGGACTGGGCCGCCTCCAAGATCTCAGGATGGACCCAAGCTCTGCCG
GACATGGTGGTGTCCCACCTTTTTGGGAAGGAAGAAATGCAGAGTAACGT
GGAAGTGGTCCACACCTACCGCCAGCACATTGTGAATGACATGAACCCCG
GCAACCTGCACCTGTTCATCAATGCCTACAACAGCCGGCGCGACCTGGAG
ATTGAGCGACCAATGCCGGGAACCCACACAGTCACCCTGCAGTGCCCTGC
TCTGTTGGTGGTTGGGGACAGCTCGCCTGCAGTGGATGCCGTGGTGGAGT
GCAACTCAAAATTGGACCCAACAAAGACCACTCTCCTCAAGATGGCGGAC
TGTGGCGGCCTCCCGCAGATCTCCCAGCCGGCCAAGCTCGCTGAGGCCTT
CAAGTACTTCGTGCAGGGCATGGGATACATGCCCTCGGCTAGCATGACCC
GCCTGATGCGGTCCCGCACAGCCTCTGGTTCCAGCGTCACTTCTCTGGAT
GGCACCCGCAGCCGCTCCCACGCCAGCGAGGGCACCCGAAGCCGCTCCCA
CACCAGCGAGGGCACCCGCAGCCGCTCGCACACCAGCGAGGGGGCCCACC
TGGACATCACCCCCAACTCGGGTGCTGCTGGGAACAGCGCCGGGCCCAAG
TCCATGGAGGTCTCCTGCTAGGGATCC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLVPRGSATMDYKDDDDKSREMQDVDLAEVK
PLVEKGETITGLLQEFDVQEQDIETLHGSVHVTLCGTPKGNRPVILTYHD
IGMNHKTCYNPLFNYEDMQEITQHFAVCHVDAPGQQDGAASFPAGYMYPS
MDQLAEMLPGVLQQFGLKSIIGMGTGAGAYILTRFALNNPEMVEGLVLIN
VNPCAEGWMDWAASKISGWTQALPDMVVSHLFGKEEMQSNVEVVHTYRQH
IVNDMNPGNLHLFINAYNSRRDLEIERPMPGTHTVTLQCPALLVVGDSSP
AVDAVVECNSKLDPTKTTLLKMADCGGLPQISQPAKLAEAFKYFVQGMGY
MPSASMTRLMRSRTASGSSVTSLDGTRSRSHASEGTRSRSHTSEGTRSRS
HTSEGAHLDITPNSGAAGNSAGPKSMEVSC*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU3193
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N terminal GST/FLAG for mammalian expression.