cDNA Clone L Plastin

• Plasmid: pGEX6P-1-L Plastin
• Parent Plasmid: pGEX6P1
• Expressed: GST-L Plastin
• Genbank: A35836
• Tag: GST
• Species: Human

Sequence of Insert

GGATCCGCCACCATGGCCAGAGGATCAGTGTCCGATGAGGAAATGATGGA
GCTCAGAGAAGCTTTTGCCAAAGTTGATACTGATGGCAATGGATACATCA
GCTTCAATGAGTTGAATGACTTGTTCAAGGCTGCTTGCTTGCCTTTGCCT
GGGTATAGAGTACGAGAAATTACAGAAAACCTGATGGCTACAGGTGATCT
GGACCAAGATGGAAGGATCAGCTTTGATGAGTTTATCAAGATTTTCCATG
GCCTAAAAAGCACAGATGTTGCCAAGACCTTTAGAAAAGCAATCAATAAG
AAGGAAGGGATTTGTGCAATCGGTGGTACTTCAGAGCAGTCTAGCGTTGG
CACCCAACACTCCTATTCAGAGGAAGAAAAGTATGCCTTTGTCAACTGGA
TAAACAAAGCCCTGGAAAATGATCCTGATTGTCGGCATGTCATCCCAATG
AACCCAAACACGAATGATCTCTTTAATGCTGTTGGAGATGGCATTGTCCT
TTGTAAAATGATCAACCTGTCAGTGCCAGACACAATTGATGAAAGAACAA
TCAACAAAAAGAAGCTAACCCCTTTCACCATTCAGGAAAATCTGAACTTG
GCTCTGAACTCTGCCTCAGCCATCGGGTGCCATGTGGTCAACATAGGGGC
TGAGGACCTGAAGGAGGGGAAGCCTTATCTGGTCCTGGGACTTCTGTGGC
AAGTCATCAAGATTGGGTTGTTTGCTGACATTGAACTCAGCAGAAATGAA
GCTCTGATTGCTCTTTTGAGAGAAGGTGAGAGCCTGGAGGATTTGATGAA
ACTCTCCCCTGAAGAGCTCTTGCTGAGGTGGGCTAATTACCACCTGGAAA
ATGCAGGCTGCAACAAAATTGGCAACTTCAGTACTGACATCAAGGACTCA
AAAGCTTATTACCACCTGCTTGAGCAGGTGGCTCCAAAAGGAGATGAAGA
AGGTGTTCCTGCTGTTGTTATTGACATGTCAGGACTGCGGGAGAAGGATG
ACATCCAGAGGGCAGAATGCATGCTGCAGCAGGCGGAGAGGCTGGGCTGC
CGGCAGTTTGTCACAGCCACAGATGTTGTCCGAGGGAACCCCAAGTTGAA
CTTGGCTTTTATTGCCAACCTCTTTAACAGATACCCTGCCCTGCACAAAC
CAGAGAACCAGGACATTGACTGGGGGGCTCTTGAAGGTGAGACGAGAGAA
GAGCGGACATTTAGGAACTGGATGAACTCCCTGGGTGTTAACCCTCGAGT
CAATCATTTGTACAGTGACTTATCAGATGCCCTGGTCATCTTCCAGCTCT
ATGAAAAGATCAAAGTTCCTGTTGACTGGAACAGAGTAAACAAACCGCCA
TACCCCAAACTGGGAGGCAATATGAAGAAGCTTGAGAATTGTAACTACGC
GGTAGAATTGGGGAAGAATCAAGCGAAGTTCTCCCTGGTTGGCATCGGTG
GACAAGATCTCAATGAAGGAAACCGCACTCTCACACTGGCCTTGATTTGG
CAGCTAATGAGAAGGTATACACTGAATATCCTCGAAGAAATTGGTGGTGG
CCAGAAGGTCAATGATGACATTATTGTCAACTGGGTGAATGAAACATTGA
GGGAAGCAGAGAAAAGTTCATCCATCTCTAGTTTCAAGGACCCGAAGATT
AGTACAAGTCTGCCTGTTCTGGACCTCATCGATGCCATCCAACCAGGTTC
CATTAACTATGACCTTCTGAAGACAGAAAATCTGAATGATGATGAGAAAC
TCAACAATGCAAAATATGCCATCTCTATGGCCCGAAAAATTGGAGCAAGA
GTGTATGCCCTGCCAGAAGACCTGGTTGAAGTGAACCCCAAAATGGTCAT
GACCGTGTTTGCCTGCCTCATGGGGAAAGGAATGAAGAGGGTGTGAGGAT
CC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSATMARGSVSDEEMMELREA
FAKVDTDGNGYISFNELNDLFKAACLPLPGYRVREITENLMATGDLDQDG
RISFDEFIKIFHGLKSTDVAKTFRKAINKKEGICAIGGTSEQSSVGTQHS
YSEEEKYAFVNWINKALENDPDCRHVIPMNPNTNDLFNAVGDGIVLCKMI
NLSVPDTIDERTINKKKLTPFTIQENLNLALNSASAIGCHVVNIGAEDLK
EGKPYLVLGLLWQVIKIGLFADIELSRNEALIALLREGESLEDLMKLSPE
ELLLRWANYHLENAGCNKIGNFSTDIKDSKAYYHLLEQVAPKGDEEGVPA
VVIDMSGLREKDDIQRAECMLQQAERLGCRQFVTATDVVRGNPKLNLAFI
ANLFNRYPALHKPENQDIDWGALEGETREERTFRNWMNSLGVNPRVNHLY
SDLSDALVIFQLYEKIKVPVDWNRVNKPPYPKLGGNMKKLENCNYAVELG
KNQAKFSLVGIGGQDLNEGNRTLTLALIWQLMRRYTLNILEEIGGGQKVN
DDIIVNWVNETLREAEKSSSISSFKDPKISTSLPVLDLIDAIQPGSINYD
LLKTENLNDDEKLNNAKYAISMARKIGARVYALPEDLVEVNPKMVMTVFA
CLMGKGMKRV*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU3144
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N terminal GST tag for bacterial expresssion.