cDNA Clone ERK5

• Synonyms: MAPK7
• Plasmid: pCMV5-HA ERK5 new
• Parent Plasmid: pCMV5
• Expressed: HA-ERK5
• Genbank: NP_002740
• Tag: HA
• Species: Human

Sequence of Insert

GGATCCGCCACCATGTACCCATACGATGTGCCAGATTACGCCGCCGAGCC
TCTGAAGGAGGAAGACGGCGAGGACGGCTCTGCGGAGCCCCCCGGGCCCG
TGAAGGCCGAACCCGCCCACACCGCTGCCTCTGTAGCGGCCAAGAACCTG
GCCCTGCTTAAAGCCCGCTCCTTCGATGTGACCTTTGACGTGGGCGACGA
GTACGAGATCATCGAGACCATAGGCAACGGGGCCTATGGAGTGGTGTCCT
CCGCCCGCCGCCGCCTCACCGGCCAGCAGGTGGCCATCAAGAAGATCCCT
AATGCTTTCGATGTGGTGACCAATGCCAAGCGGACCCTCAGGGAGCTGAA
GATCCTCAAGCACTTTAAACACGACAACATCATCGCCATCAAGGACATCC
TGAGGCCCACCGTGCCCTATGGCGAATTCAAATCTGTCTACGTGGTCCTG
GACCTGATGGAAAGCGACCTGCACCAGATCATCCACTCCTCACAGCCCCT
CACACTGGAACACGTGCGCTACTTCCTGTACCAACTGCTGCGGGGCCTGA
AGTACATGCACTCGGCTCAGGTCATCCACCGTGACCTGAAGCCCTCCAAC
CTATTGGTGAATGAGAACTGTGAGCTCAAGATTGGTGACTTTGGTATGGC
TCGTGGCCTGTGCACCTCGCCCGCTGAACATCAGTACTTCATGACTGAGT
ATGTGGCCACGCGCTGGTACCGTGCGCCCGAGCTCATGCTCTCTTTGCAT
GAGTATACACAGGCTATTGACCTCTGGTCTGTGGGCTGCATCTTTGGTGA
GATGCTGGCCCGGCGCCAGCTCTTCCCAGGCAAAAACTATGTACACCAGC
TACAGCTCATCATGATGGTGCTGGGTACCCCATCACCAGCCGTGATTCAG
GCTGTGGGGGCTGAGAGGGTGCGGGCCTATATCCAGAGCTTGCCACCACG
CCAGCCTGTGCCCTGGGAGACAGTGTACCCAGGTGCCGACCGCCAGGCCC
TATCACTGCTGGGTCGCATGCTGCGTTTTGAGCCCAGCGCTCGCATCTCA
GCAGCTGCTGCCCTTCGCCACCCTTTCCTGGCCAAGTACCATGATCCTGA
TGATGAGCCTGACTGTGCCCCGCCCTTTGACTTTGCCTTTGACCGCGAAG
CCCTCACTCGGGAGCGCATTAAGGAGGCCATTGTGGCTGAAATTGAGGAC
TTCCATGCAAGGCGTGAGGGCATCCGCCAACAGATCCGCTTCCAGCCTTC
TCTACAGCCTGTGGCTAGTGAGCCTGGCTGTCCAGATGTTGAAATGCCCA
GTCCCTGGGCTCCCAGTGGGGACTGTGCCATGGAGTCTCCACCACCAGCC
CCGCCACCATGCCCCGGCCCTGCACCTGACACCATTGATCTGACCCTGCA
GCCACCTCCACCAGTCAGTGAGCCTGCCCCACCAAAGAAAGATGGTGCCA
TCTCAGACAATACTAAGGCTGCCCTTAAAGCTGCCCTGCTCAAGTCTTTG
AGGAGCCGGCTCAGAGATGGCCCCAGCGCACCCCTGGAGGCTCCTGAGCC
TCGGAAGCCGGTGACAGCCCAGGAGCGCCAGCGGGAGCGGGAGGAGAAGC
GGCGGAGGCGGCAAGAACGAGCCAAGGAGCGGGAGAAACGGCGGCAGGAG
CGGGAGCGAAAGGAACGGGGGGCTGGGGCCTCTGGGGGCCCCTCCACTGA
CCCCTTGGCTGGACTAGTGCTCAGTGACAATGACAGAAGCCTGTTGGAAC
GCTGGACTCGAATGGCCCGGCCCGCAGCCCCAGCCCTCACCTCTGTGCCG
GCCCCTGCCCCAGCGCCAACGCCAACCCCAACCCCAGTCCAACCTACCAG
TCCTCCTCCTGGCCCTGTAGCCCAGCCCACTGGCCCGCAACCACAATCTG
CGGGCTCTACCTCTGGCCCTGTACCCCAGCCTGCCTGCCCACCCCCTGGC
CCTGCACCCCACCCCACTGGCCCTCCTGGGCCCATCCCTGTCCCCGCGCC
ACCCCAGATTGCCACCTCCACCAGCCTCCTGGCTGCCCAGTCACTTGTGC
CACCCCCTGGGCTGCCTGGCTCCAGCACCCCAGGAGTTTTGCCTTACTTC
CCACCTGGCCTGCCGCCCCCAGACGCCGGGGGAGCCCCTCAGTCTTCCAT
GTCAGAGTCACCTGATGTCAACCTTGTGACCCAGCAGCTATCTAAGTCAC
AGGTGGAGGACCCCCTGCCCCCTGTGTTCTCAGGCACACCAAAGGGCAGT
GGGGCTGGCTACGGTGTTGGCTTTGACCTGGAGGAATTCTTAAACCAGTC
TTTCGACATGGGCGTGGCTGATGGGCCACAGGATGGCCAGGCAGATTCAG
CCTCTCTCTCAGCCTCCCTGCTTGCTGACTGGCTCGAAGGCCATGGCATG
AACCCTGCCGATATTGAGTCCCTGCAGCGTGAGATCCAGATGGACTCCCC
AATGCTGCTGGCTGACCTGCCTGACCTCCAGGACCCCTGAGGATCC

Amino Acid Sequence

MYPYDVPDYAAEPLKEEDGEDGSAEPPGPVKAEPAHTAASVAAKNLALLK
ARSFDVTFDVGDEYEIIETIGNGAYGVVSSARRRLTGQQVAIKKIPNAFD
VVTNAKRTLRELKILKHFKHDNIIAIKDILRPTVPYGEFKSVYVVLDLME
SDLHQIIHSSQPLTLEHVRYFLYQLLRGLKYMHSAQVIHRDLKPSNLLVN
ENCELKIGDFGMARGLCTSPAEHQYFMTEYVATRWYRAPELMLSLHEYTQ
AIDLWSVGCIFGEMLARRQLFPGKNYVHQLQLIMMVLGTPSPAVIQAVGA
ERVRAYIQSLPPRQPVPWETVYPGADRQALSLLGRMLRFEPSARISAAAA
LRHPFLAKYHDPDDEPDCAPPFDFAFDREALTRERIKEAIVAEIEDFHAR
REGIRQQIRFQPSLQPVASEPGCPDVEMPSPWAPSGDCAMESPPPAPPPC
PGPAPDTIDLTLQPPPPVSEPAPPKKDGAISDNTKAALKAALLKSLRSRL
RDGPSAPLEAPEPRKPVTAQERQREREEKRRRRQERAKEREKRRQERERK
ERGAGASGGPSTDPLAGLVLSDNDRSLLERWTRMARPAAPALTSVPAPAP
APTPTPTPVQPTSPPPGPVAQPTGPQPQSAGSTSGPVPQPACPPPGPAPH
PTGPPGPIPVPAPPQIATSTSLLAAQSLVPPPGLPGSSTPGVLPYFPPGL
PPPDAGGAPQSSMSESPDVNLVTQQLSKSQVEDPLPPVFSGTPKGSGAGY
GVGFDLEEFLNQSFDMGVADGPQDGQADSASLSASLLADWLEGHGMNPAD
IESLQREIQMDSPMLLADLPDLQDP*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU3127
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N terminal HA tag for mammalian expression.