cDNA Clone PPM1B

• Synonyms: PP2C-beta; PP2C-beta-X; PP2CB; PP2CBETA; PPC2BETAX
• Plasmid: pGEX6P1 PPM1B
• Parent Plasmid: pGEX6P1
• Expressed: GST 3C PPM1B
• Genbank: AAH64381 NM_002706.6
• Tag: GST
• Species: Human

Sequence of Insert

GAATTCGGTGCATTTTTGGATAAACCCAAAACTGAAAAACATAATGCTCA
TGGTGCTGGGAATGGTTTACGTTATGGCCTGAGCAGCATGCAAGGATGGA
GAGTGGAAATGGAAGATGCACACACAGCTGTTGTAGGTATTCCTCACGGC
TTGGAAGACTGGTCATTTTTTGCAGTTTATGATGGTCATGCTGGATCCCG
AGTGGCAAATTACTGCTCAACACATTTATTAGAACACATCACTACTAACG
AAGACTTTAGGGCAGCTGGAAAATCAGGATCTGCTCTTGAGCTTTCAGTG
GAAAATGTTAAGAATGGTATCAGAACTGGATTTTTGAAAATTGATGAATA
CATGCGTAACTTTTCAGACCTCAGAAACGGGATGGACAGGAGTGGTTCAA
CTGCAGTGGGAGTTATGATTTCACCTAAGCATATCTACTTTATCAACTGT
GGTGATTCACGTGCTGTTCTGTATAGGAATGGACAAGTCTGCTTTTCTAC
CCAGGATCACAAACCTTGCAATCCAAGGGAAAAGGAGCGAATCCAAAATG
CAGGAGGCAGCGTGATGATACAACGTGTTAATGGTTCATTAGCAGTATCT
CGTGCTCTGGGGGACTATGATTACAAGTGTGTTGATGGCAAGGGCCCAAC
AGAACAACTTGTTTCTCCAGAGCCTGAGGTTTATGAAATTTTAAGAGCAG
AAGAGGATGAATTTATCATCTTGGCTTGTGATGGGATCTGGGATGTTATG
AGTAATGAGGAGCTCTGTGAATATGTTAAATCTAGGCTTGAGGTATCTGA
TGACCTGGAAAATGTGTGCAATTGGGTAGTGGACACTTGTTTACACAAGG
GAAGTCGAGATAACATGAGTATTGTACTAGTTTGCTTTTCAAATGCTCCC
AAGGTCTCAGATGAAGCGGTGAAAAAAGATTCAGAGTTGGATAAGCACTT
GGAATCACGGGTTGAAGAGATTATGGAGAAGTCTGGCGAGGAAGGAATGC
CTGATCTTGCCCATGTCATGCGCATCTTGTCTGCAGAAAATATCCCAAAT
TTGCCTCCTGGGGGAGGTCTTGCTGGCAAGCGTAATGTTATTGAAGCTGT
TTATAGTAGACTGAATCCACATAGAGAAAGTGATGGGGCCTCCGATGAAG
CAGAGGAAAGTGGATCACAGGGAAAATTGGTGGAAGCTCTCAGGCAAATG
AGAATTAATCATAGGGGAAACTACCGACAACTTCTGGAGGAGATGCTGAC
TAGTTACAGGCTAGCTAAAGTAGAGGGAGAAGAAAGCCCTGCTGAACCAG
CTGCCACAGCTACTTCTTCGAACAGTGATGCTGGAAACCCAGTGACAATG
CAGGAAAGCCATACTGAATCAGAAAGTGGTCTTGCTGAATTAGACAGCTC
TAATGAAGATGCAGGGACAAAGATGAGTGGTGAAAAAATATGAGCGGCCG
C

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSPEFGAFLDKPKTEKHNAHG
AGNGLRYGLSSMQGWRVEMEDAHTAVVGIPHGLEDWSFFAVYDGHAGSRV
ANYCSTHLLEHITTNEDFRAAGKSGSALELSVENVKNGIRTGFLKIDEYM
RNFSDLRNGMDRSGSTAVGVMISPKHIYFINCGDSRAVLYRNGQVCFSTQ
DHKPCNPREKERIQNAGGSVMIQRVNGSLAVSRALGDYDYKCVDGKGPTE
QLVSPEPEVYEILRAEEDEFIILACDGIWDVMSNEELCEYVKSRLEVSDD
LENVCNWVVDTCLHKGSRDNMSIVLVCFSNAPKVSDEAVKKDSELDKHLE
SRVEEIMEKSGEEGMPDLAHVMRILSAENIPNLPPGGGLAGKRNVIEAVY
SRLNPHRESDGASDEAEESGSQGKLVEALRQMRINHRGNYRQLLEEMLTS
YRLAKVEGEESPAEPAATATSSNSDAGNPVTMQESHTESESGLAELDSSN
EDAGTKMSGEKI*

• Vector Type: Bacterial
• Antibiotic: Amp
• Full Snapgene Map: pGEX6P1 PPM1B57.42 KB Snapgene files can be opened using software available here: www.snapgene.com
• DU Number: DU3021
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N terminal GST for bacterial expression.
• Low Temp: No