cDNA Clone PPM1g

• Synonyms: PP2C Gamma
• Plasmid: pGEX6P-1-PPM1g
• Parent Plasmid: pGEX6P1
• Expressed: GST-PPM1g
• Genbank: O15355 NM_177983.3 → NP_817092.1
• Tag: GST
• Species: Human

Sequence of Insert

GGATCCGGTGCCTACCTCTCCCAGCCCAACACGGTGAAGTGCTCCGGGGA
CGGGGTCGGCGCCCCGCGCCTGCCGCTGCCCTACGGCTTCTCCGCCATGC
AAGGCTGGCGCGTCTCCATGGAGGATGCTCACAACTGTATTCCTGAGCTG
GACAGTGAGACAGCCATGTTTTCTGTCTACGATGGACATGGAGGGGAGGA
AGTTGCCTTGTACTGTGCCAAATATCTTCCTGATATCATCAAAGATCAGA
AGGCCTACAAGGAAGGCAAGCTACAGAAGGCTTTAGAAGATGCCTTCTTG
GCTATTGACGCCAAATTGACCACTGAAGAAGTCATTAAAGAGCTGGCACA
GATTGCAGGGCGACCCACTGAGGATGAAGATGAAAAAGAAAAAGTAGCTG
ATGAAGATGATGTGGACAATGAGGAGGCTGCACTGCTGCATGAAGAGGCT
ACCATGACTATTGAAGAGCTGCTGACACGCTACGGGCAGAACTGTCACAA
GGGCCCTCCCCACAGCAAATCTGGAGGTGGGACAGGCGAGGAACCAGGGT
CCCAGGGCCTCAATGGGGAGGCAGGACCTGAGGACTCAACTAGGGAAACT
CCTTCACAAGAAAATGGCCCCACAGCCAAGGCCTACACAGGCTTTTCCTC
CAACTCGGAACGTGGGACTGAGGCAGGCCAAGTTGGTGAGCCTGGCATTC
CCACTGGTGAGGCTGGGCCTTCCTGCTCTTCAGCCTCTGACAAGCTGCCT
CGAGTTGCTAAGTCCAAGTTCTTTGAGGACAGTGAGGATGAGTCAGATGA
GGCGGAGGAAGAAGAGGAAGACAGTGAGGAATGCAGCGAGGAAGAGGATG
GCTACAGCAGTGAGGAGGCAGAGAATGAGGAAGATGAGGATGACACCGAG
GAGGCTGAAGAGGACGATGAAGAAGAAGAAGAAGAGATGATGGTGCCAGG
GATGGAAGGCAAAGAGGAGCCTGGCTCTGACAGTGGTACAACAGCGGTGG
TGGCCCTGATACGAGGGAAGCAGTTGATTGTAGCCAACGCAGGAGACTCT
CGCTGTGTGGTATCTGAGGCTGGCAAAGCTTTAGACATGTCCTATGATCA
CAAACCAGAGGATGAAGTAGAACTAGCACGCATCAAGAATGCTGGTGGCA
AGGTCACCATGGATGGGCGAGTCAACGGGGGCCTCAACCTCTCCAGAGCC
ATTGGGGACCACTTCTATAAGAGAAACAAGAACCTGCCACCTGAGGAACA
GATGATTTCAGCCCTTCCTGACATCAAGGTGCTGACTCTCACTGACGACC
ATGAATTCATGGTCATTGCCTGTGATGGCATCTGGAATGTGATGAGCAGC
CAGGAAGTTGTAGATTTCATTCAATCAAAGATCAGCCAGCGTGATGAAAA
TGGGGAGCTTCGGTTATTGTCATCCATTGTGGAAGAGCTGCTGGATCAGT
GCCTGGCACCAGACACTTCTGGGGATGGTACAGGGTGTGACAACATGACC
TGCATCATCATTTGCTTCAAGCCCCGAAACACAGCAGAGCTCCAGCCAGA
GAGTGGCAAGCGAAAACTAGAGGAGGTGCTCTCTACTGAGGGGGCTGAAG
AAAATGGCAACAGCGACAAGAAGAAGAAGGCCAAGCGAGACTAGGCGGCC
GC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSGAYLSQPNTVKCSGDGVGA
PRLPLPYGFSAMQGWRVSMEDAHNCIPELDSETAMFSVYDGHGGEEVALY
CAKYLPDIIKDQKAYKEGKLQKALEDAFLAIDAKLTTEEVIKELAQIAGR
PTEDEDEKEKVADEDDVDNEEAALLHEEATMTIEELLTRYGQNCHKGPPH
SKSGGGTGEEPGSQGLNGEAGPEDSTRETPSQENGPTAKAYTGFSSNSER
GTEAGQVGEPGIPTGEAGPSCSSASDKLPRVAKSKFFEDSEDESDEAEEE
EEDSEECSEEEDGYSSEEAENEEDEDDTEEAEEDDEEEEEEMMVPGMEGK
EEPGSDSGTTAVVALIRGKQLIVANAGDSRCVVSEAGKALDMSYDHKPED
EVELARIKNAGGKVTMDGRVNGGLNLSRAIGDHFYKRNKNLPPEEQMISA
LPDIKVLTLTDDHEFMVIACDGIWNVMSSQEVVDFIQSKISQRDENGELR
LLSSIVEELLDQCLAPDTSGDGTGCDNMTCIIICFKPRNTAELQPESGKR
KLEEVLSTEGAEENGNSDKKKKAKRD*

• Vector Type: Bacterial
• Antibiotic: Amp
• Full Snapgene Map: pGEX6P-1-PPM1g73.84 KB Snapgene files can be opened using software available here: www.snapgene.com
• DU Number: DU3019
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N terminal GST for bacterial expression.
• Low Temp: No