cDNA Clone Cyclin G1

• Plasmid: pGEX6P-1-Cyclin g1
• Parent Plasmid: pGEX6P1
• Expressed: GST-Cyclin g1
• Genbank: 2210321A
• Tag: GST
• Species: Human

Sequence of Insert

GGATCCATAGAGGTACTGACAACAACTGACTCTCAGAAACTGCTACACCA
GCTGAATGCCCTGTTGGAACAGGAGTCTAGATGTCAGCCAAAGGTCTGTG
GTTTGAGACTAATTGAGTCTGCACACGATAATGGCCTCAGAATGACTGCA
AGACTAAGGGACTTTGAAGTAAAAGATCTTCTTAGTCTAACTCAGTTCTT
TGGCTTTGACACAGAGACATTTTCTCTAGCTGTGAATTTACTGGACAGAT
TCCTGTCTAAAATGAAGGTACAGCCCAAGCACCTTGGGTGTGTTGGACTG
AGCTGCTTTTATTTGGCTGTAAAATCAATAGAAGAGGAAAGGAATGTCCC
ATTGGCAACTGACTTGATCCGAATAAGTCAATATAGGTTTACGGTTTCAG
ACTTGATGAGAATGGAAAAGATTGTATTGGAGAAGGTGTGTTGGAAAGTC
AAAGCTACTACTGCCTTTCAATTTCTGCAACTGTATTATTCACTCCTTCA
AGAGAACTTGCCACTTGAAAGGAGAAATAGCATTAATTTTGAAAGACTAG
AAGCTCAACTGAAGGCATGTCATTGCAGGATCATATTTTCTAAAGCAAAG
CCTTCTGTGTTGGCATTGTCTATCATTGCATTAGAGATCCAAGCACAGAA
GTGTGTAGAGTTAACAGAAGGAATAGAATGTCTTCAGAAACATTCCAAGA
TAAATGGCAGAGATCTGACCTTCTGGCAAGAGCTTGTATCCAAATGTTTA
ACTGAATATTCATCAAATAAGTGTTCCAAACCAAATGTTCAGAAGTTGAA
ATGGATTGTTTCTGGGCGTACTGCACGGCAATTGAAGCATAGCTACTACA
GAATAACTCACCTTCCAACAATTCCTGAAATGGTCCCTTAAGAATTC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSIEVLTTTDSQKLLHQLNAL
LEQESRCQPKVCGLRLIESAHDNGLRMTARLRDFEVKDLLSLTQFFGFDT
ETFSLAVNLLDRFLSKMKVQPKHLGCVGLSCFYLAVKSIEEERNVPLATD
LIRISQYRFTVSDLMRMEKIVLEKVCWKVKATTAFQFLQLYYSLLQENLP
LERRNSINFERLEAQLKACHCRIIFSKAKPSVLALSIIALEIQAQKCVEL
TEGIECLQKHSKINGRDLTFWQELVSKCLTEYSSNKCSKPNVQKLKWIVS
GRTARQLKHSYYRITHLPTIPEMVP*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU3018
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N terminal GST for bacterial expression.