cDNA Clone EF2 Kinase

• Plasmid: pGEX6P-1-EF2K human new
• Parent Plasmid: pGEX6P1
• Expressed: GST-EF2K human new
• Genbank: BC032665
• Tag: GST
• Species: Human

Sequence of Insert

GGATCCGCAGACGAAGACCTCATCTTCCGCCTGGAAGGCGTTGATGGCGG
CCAGTCCCCCCGAGCTGGCCGTGATGGTGATTCTGATGGGGACAGCGACG
ATGAGGAAGGTTACTTCATCTGCCCCATCACGGATGACCCAAGCTCGAAC
CAGAATGTCAATTCCAAGGTTAATAAGTACTACAGCAACCTAACAAAAAG
TGAGCGGTATAGCTCCAGCGGGTCCCCGGCAAACTCCTTCCACTTCAAGG
AAGCCTGGAAGCACGCAATCCAGAAGGCCAAGCACATGCCCGACCCCTGG
GCTGAGTTCCACCTGGAAGATATTGCCACCGAACGTGCTACTCGACACAG
GTACAACGCCGTCACCGGGGAATGGCTGGATGATGAAGTTCTGATCAAGA
TGGCATCTCAGCCCTTCGGCCGAGGAGCAATGAGGGAGTGCTTCCGGACG
AAGAAGCTCTCCAACTTCTTGCATGCCCAGCAGTGGAAGGGAGCCTCCAA
CTACGTGGCGAAGCGCTACATCGAGCCCGTAGACCGGGATGTGTACTTTG
AGGACGTGCGTCTACAGATGGAGGCCAAGCTCTGGGGGGAGGAGTATAAT
CGGCACAAGCCCCCCAAGCAGGTGGACATCATGCAGATGTGCATCATCGA
GCTGAAGGACAGACCGGGCAAGCCCCTCTTCCACCTGGAGCACTACATCG
AGGGCAAGTACATCAAGTACAACTCCAACTCTGGCTTTGTCCGCGATGAC
AACATCCGCCTGACGCCGCAGGCCTTCAGCCACTTCACTTTTGAGCGTTC
CGGCCATCAGCTGATAGTGGTGGACATCCAGGGAGTTGGGGATCTCTACA
CTGACCCACAGATCCACACGGAGACGGGCACTGACTTTGGAGACGGCAAC
CTAGGTGTCCGCGGGATGGCGCTCTTCTTCTACTCTCATGCCTGCAACCG
GATTTGCGAGAGCATGGGCCTTGCTCCCTTTGACCTCTCGCCCCGGGAGA
GGGATGCAGTGAATCAGAACACCAAGCTGCTGCAATCAGCCAAGACCATC
TTGAGAGGAACAGAGGAAAAATGTGGGAGCCCCCGAGTAAGGACCCTCTC
TGGGAGCCGGCCACCCCTGCTCCGTCCCCTTTCAGAGAACTCTGGAGACG
AGAACATGAGCGACGTGACCTTCGACTCTCTCCCTTCTTCCCCATCTTCG
GCCACACCACACAGCCAGAAGCTAGACCACCTCCATTGGCCAGTGTTCAG
TGACCTCGATAACATGGCATCCAGAGACCATGATCATCTAGACAACCACC
GGGAGTCTGAGAATAGTGGGGACAGCGGATACCCCAGTGAGAAGCGGGGT
GAGCTGGATGACCCTGAGCCCCGAGAACATGGCCACTCATACAGTAATCG
GAAGTACGAGTCTGACGAAGACAGCCTGGGCAGCTCTGGACGGGTATGTG
TAGAGAAGTGGAATCTCCTCAACTCCTCCCGCCTCCACCTGCCGAGGGCT
TCGGCCGTGGCCCTGGAAGTGCAAAGGCTTAATGCTCTGGACCTCGAAAA
GAAAATCGGGAAGTCCATTTTGGGGAAGGTCCATCTGGCCATGGTGCGCT
ACCACGAGGGTGGGCGCTTCTGCGAGAAGGGCGAGGAGTGGGACCAGGAG
TCGGCTGTCTTCCACCTGGAGCACGCAGCCAACCTGGGCGAGCTGGAGGC
CATCGTGGGCCTGGGACTCATGTACTCGCAGTTGCCTCATCACATCCTAG
CCGATGTCTCTCTGAAGGAGACAGAAGAGAACAAAACCAAAGGATTTGAT
TACTTACTAAAGGCCGCTGAAGCTGGCGACAGGCAGTCCATGATCCTAGT
GGCGCGAGCTTTTGACTCTGGCCAGAACCTCAGCCCGGACAGGTGCCAAG
ACTGGCTAGAGGCCCTGCACTGGTACAACACTGCCCTGGAGATGACGGAC
TGTGATGAGGGCGGTGAGTACGACGGAATGCAGGACGAGCCCCGGTACAT
GATGCTGGCCAGGGAGGCCGAGATGCTGTTCACAGGAGGCTACGGGCTGG
AGAAGGACCCGCAGAGATCAGGGGACTTGTATACCCAGGCAGCAGAGGCA
GCGATGGAAGCCATGAAGGGCCGACTGGCCAACCAGTACTACCAAAAGGC
TGAAGAGGCCTGGGCCCAGATGGAGGAATAAGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSADEDLIFRLEGVDGGQSPR
AGRDGDSDGDSDDEEGYFICPITDDPSSNQNVNSKVNKYYSNLTKSERYS
SSGSPANSFHFKEAWKHAIQKAKHMPDPWAEFHLEDIATERATRHRYNAV
TGEWLDDEVLIKMASQPFGRGAMRECFRTKKLSNFLHAQQWKGASNYVAK
RYIEPVDRDVYFEDVRLQMEAKLWGEEYNRHKPPKQVDIMQMCIIELKDR
PGKPLFHLEHYIEGKYIKYNSNSGFVRDDNIRLTPQAFSHFTFERSGHQL
IVVDIQGVGDLYTDPQIHTETGTDFGDGNLGVRGMALFFYSHACNRICES
MGLAPFDLSPRERDAVNQNTKLLQSAKTILRGTEEKCGSPRVRTLSGSRP
PLLRPLSENSGDENMSDVTFDSLPSSPSSATPHSQKLDHLHWPVFSDLDN
MASRDHDHLDNHRESENSGDSGYPSEKRGELDDPEPREHGHSYSNRKYES
DEDSLGSSGRVCVEKWNLLNSSRLHLPRASAVALEVQRLNALDLEKKIGK
SILGKVHLAMVRYHEGGRFCEKGEEWDQESAVFHLEHAANLGELEAIVGL
GLMYSQLPHHILADVSLKETEENKTKGFDYLLKAAEAGDRQSMILVARAF
DSGQNLSPDRCQDWLEALHWYNTALEMTDCDEGGEYDGMQDEPRYMMLAR
EAEMLFTGGYGLEKDPQRSGDLYTQAAEAAMEAMKGRLANQYYQKAEEAW
AQMEE*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU3011
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N terminal GST for bacterial expression.