cDNA Clone DYRK1A (rat)

• Plasmid: pGFP DYRK
• Parent Plasmid: pEGFPC1
• Expressed: GFP DYRK
• Genbank: X79769
• Tag: GFP
• Species: Rat

Sequence of Insert

AGATCTCAGACGATGCATACAGGAGGAGAGACTTCAGCATGCAAACCTTC
ATCTGTCCGGCTCGCACCGTCATTCTCATTCCATGCTGCTGGCCTTCAGA
TGGCTGCACAGATGCCCCATTCACATCAGTACAGTGACCGTCGCCAGCCA
AACATAAGTGACCAGCAGGTTTCTGCCTTATCATATTCTGACCAGATTCA
GCAACCTCTAACTAACCAGGTGATGCCTGACATTGTCATGTTACAGAGGC
GGATGCCCCAAACCTTCCGTGATCCAGCAACTGCTCCTCTGAGAAAACTC
TCTGTGGACTTGATCAAAACATACAAGCATATTAATGAGGTTTACTATGC
AAAAAAGAAGCGAAGACACCAACAGGGCCAGGGGGACGATTCTAGTCATA
AGAAGGAGCGGAAGGTTTACAACGATGGTTACGATGACGATAACTATGAT
TACATTGTAAAAAACGGAGAAAAGTGGATGGACCGTTATGAAATCGACTC
CTTAATAGGCAAAGGTTCATTTGGACAGGTTGTGAAAGCATATGACAGAG
TGGAACAAGAATGGGTCGCCATTAAAATCATCAAGAACAAGAAGGCGTTT
CTGAATCAAGCGCAGATAGAAGTGCGGCTGCTTGAGCTCATGAACAAACA
TGACACTGAGATGAAGTACTACATAGTGCATTTGAAACGCCACTTTATGT
TTCGGAACCATCTCTGTCTAGTGTTTGAAATGCTGTCCTATAATCTCTAT
GATTTGTTGAGGAACACCAACTTCCGAGGGGTCTCTTTGAACCTGACACG
AAAGTTTGCACAACAGATGTGCACAGCATTGCTTTTCCTTGCGACTCCAG
AACTTAGTATCATTCACTGTGACTTAAAGCCGGAGAACATCCTTCTGTGT
AACCCCAAACGAAGTGCAATCAAGATTGTTGACTTTGGCAGCTCTTGTCA
GTTGGGGCAGAGGATATACCAGTATATTCAGAGTCGCTTCTATCGGTCTC
CAGAGGTGCTACTGGGAATGCCTTATGACCTTGCTATCGACATGTGGTCC
CTTGGATGTATCTTGGTTGAAATGCACACTGGAGAGCCTCTGTTCAGTGG
TGCCAATGAGGTGGATCAGATGAATAAAATAGTGGAAGTCTTGGGCATCC
CACCTGCTCATATTCTTGACCAAGCACCGAAAGCAAGAAAGTTCTTTGAG
AAGTTGCCTGATGGCACTTGGAGCTTAAAGAAGACCAAAGATGGAAAACG
GGAGTACAAACCACCAGGAACCCGTAAACTTCATAATATTCTTGGAGTGG
AAACAGGAGGACCTGGCGGGCGGCGTGCTGGGGAGTCAGGTCACACTGTA
GCTGACTACTTGAAGTTCAAAGACCTCATTTTAAGGATGCTTGATTATGA
CCCCAAAACTCGGATTCAACCTTATTATGCCCTGCAGCACAGTTTTTTCA
AGAAAACAGCTGATGAAGGTACAAACACAAGCAACAGTGTGTCCACCAGC
CCTGCCATGGAGCAGTCCCAGTCTTCAGGCACCACCTCCAGCACCTCCTC
CAGCTCAGGTGGATCCTCGGGAACGAGCAACAGTGGGAGAGCCAGGTCGG
ATCCGACGCACCAGCATCGGCACAGTGGCGGGCACTTCGCTGCTGCTGTC
CAGGCCATGGACTGTGAGACACATAGTCCCCAGGTGCGCCAGCAGTTTCC
GGCTCCTCTGGGCTGGTCAGGCACTGAAGCTCCTACACAAGTCACTGTTG
AAACTCATCCTGTTCAAGAAACAACCTTTCATGTAGCCCCTCAGCAGAAT
GCATTGCATCATCACCATGGAAACAGTTCCCATCATCACCACCACCACCA
TCACCACCACCACCACCATGGACAGCAAGCCTTGGGTAACCGGACCAGGC
CAAGGGTCTACAATTCTCCAACAAATAGCTCCTCTACCCAGGATTCTATG
GAGGTTGGCCACAGTCACCACTCCATGACATCCCTGTCTTCCTCAACAAC
TTCTTCCTCGACATCTTCCTCCTCTACTGGTAATCAAGGCAATCAGGCCT
ATCAGAACCGCCCAGTGGCTGCTAACACCTTGGACTTTGGACAGAATGGA
GCTATGGACGTTAATTTGACCGTCTACTCCAATCCCCGCCAAGAGACTGG
CATAGCTGGACATCCAACATACCAATTTTCTGCTAATACAGGTCCTGCAC
ATTACATGACTGAAGGACATCTGACGATGAGGCAAGGGGCTGATAGAGAA
GAGTCTCCCATGACAGGAGTTTGTGTGCAACAGAGTCCTGTAGCTAGCTC
GTGACTACATTGAAACTTGAGTTTGTTTCTTGTGTGTTTTTATAGAAGTG
GTGTTTTTTTCCCAAAAACAAAAGTGCAAAGCTGCTTGAATCAGAAGGAG
ATTAACACACTGAACCGCTACGAGAGGGCAAAGCTGACTTTTTTTTTTAA
CTTGAAAAGATTGCAAAGGGACAATGAAGTTTTTAAGAGCCATGTCCACC
CATCTTCATGGAGCGCTCAGAGTGTCCTCTTTCTGCCTCCCCCATTTAAC
TTCCATCTCCCGGTCACAGTGGGTTTTGTCTTTCTATCCAACAAAAGTTA
ATGTTCAGCTGTTGGTCTTGGTCATTTGCCAACTAATTTTGGGATCCACC
GGATCTAGA

Amino Acid Sequence

MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICT
TGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIF
FKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHN
VYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
YLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKSGLRSQTMHTG
GETSACKPSSVRLAPSFSFHAAGLQMAAQMPHSHQYSDRRQPNISDQQVS
ALSYSDQIQQPLTNQVMPDIVMLQRRMPQTFRDPATAPLKKLSVDLIKTY
KHINEVYYAKKKRRHQQGQGDDSSHKKERKVYNDGYDDDNYDYIVKNGEK
WMDRYEIDSLIGKGSFGQVVKAYDRVEQEWVAIKIIKNKKAFLNQAQIEV
RLLELMNKHDTEMKYYIVHLKRHFMFRNHLCLVFEMLSYNLYDLLRNTNF
RGVSLNLTRKFAQQMCTALLFLATPELSIIHCDLKPENILLCNPKRSAIK
IVDFGSSCQLGQRIYQYIQSRFYRSPEVLLGMPYDLAIDMWSLGCILVEM
HTGEPLFSGANEVDQMNKIVEVLGIPPAHILDQAPKARKFFEKLPDGTWS
LKKTKDGKREYKPPGTRKLHNILGVETGGPGGRRAGESGHTVADYLKFKD
LILRMLDYDPKTRIQPYYALQHSFFKKTADEGTNTSNSVSTSPAMEQSQS
SGTTSSTSSSSGGSSGTSNSGRARSDPTHQHRHSGGHFAAAVQAMDCETH
SPQVRQQFPAPLGWSGTEAPTQVTVETHPVQETTFHVAPQQNALHHHHGN
SSHHHHHHHHHHHHHGQQALGNRTRPRVYNSPTNSSSTQDSMEVGHSHHS
MTSLSSSTTSSSTSSSSTGNQGNQAYQNRPVAANTLDFGQNGAMDVNLTV
YSNPRQETGIAGHPTYQFSANTGPAHYMTEGHLTMRQGADREESPMTGVC
VQQSPVASS*LH*NLSLFLVCFYRSGVFFPKTKVQSCLNQKEINTLNRYE
RAKLTFFFNLKRLQRDNEVFKSHVHPSSWSAQSVLFLPPPFNFHLPVTVG
FVFLSNKS*

• Vector Type: Mammalian
• Antibiotic: Kan
• DU Number: DU1950
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Mammalian expression plasmid with N terminal GFP tag encoding DYRK1a.