cDNA Clone PHK liver

• Plasmid: pGEX6P-1-PBK liver D302-407
• Parent Plasmid: pGEX6P1
• Expressed: GST-PBK liver D302-407
• Genbank: M31606
• Tag: GST
• Species: Human

Sequence of Insert

GAATTCACGCTGGACGTGGGGCCGGAGGATGAGCTGCCCGACTGGGCCGC
CGCCAAAGAGTTTTACCAGAAGTACGACCCTAAGGACGTCATCGGCAGAG
GAGTGAGCTCTGTGGTCCGCCGTTGTGTTCATCGAGCTACTGGCCACGAG
TTTGCGGTGAAGATTATGGAAGTGACAGCTGAGCGGCTGAGTCCTGAGCA
GCTGGAGGAGGTGCGGGAAGCCACACGGCGAGAGACACACATCCTTCGCC
AGGTCGCCGGCCACCCCCACATCATCACCCTCATCGATTCCTACGAGTCT
TCTAGCTTCATGTTCCTGGTGTTTGACCTGATGCGGAAGGGAGAGCTGTT
TGACTATCTCACAGAGAAGGTGGCCCTCTCTGAAAAGGAAACCAGGTCCA
TCATGCGGTCTCTGCTGGAAGCAGTGAGCTTTCTCCATGCCAACAACATT
GTGCATCGAGATCTGAAGCCCGAGAATATTCTCCTAGATGACAATATGCA
GATCCGACTTTCAGATTTCGGGTTCTCCTGCCACTTGGAACCTGGCGAGA
AGCTTCGAGAGTTGTGTGGGACCCCAGGGTATCTAGCGCCAGAGATCCTT
AAATGCTCCATGGATGAAACCCACCCAGGCTATGGCAAGGAGGTCGACCT
CTGGGCCTGTGGGGTGATCTTGTTCACACTCCTGGCTGGCTCGCCACCCT
TCTGGCACCGGCGGCAGATCCTGATGTTACGCATGATCATGGAGGGCCAG
TACCAGTTCAGTTCCCCCGAGTGGGATGACCGTTCCAGCACTGTCAAAGA
CCTGATCTCCAGGCTGCTGCAGGTGGATCCTGAGGCACGCCTGACAGCTG
AGCAGGCCCTACAGCACCCCTTCTTTGAGCGTTGTGAAGGCAGCCAACCC
TGGAACTAACTCGAG

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSPEFTLDVGPEDELPDWAAA
KEFYQKYDPKDVIGRGVSSVVRRCVHRATGHEFAVKIMEVTAERLSPEQL
EEVREATRRETHILRQVAGHPHIITLIDSYESSSFMFLVFDLMRKGELFD
YLTEKVALSEKETRSIMRSLLEAVSFLHANNIVHRDLKPENILLDDNMQI
RLSDFGFSCHLEPGEKLRELCGTPGYLAPEILKCSMDETHPGYGKEVDLW
ACGVILFTLLAGSPPFWHRRQILMLRMIMEGQYQFSSPEWDDRSSTVKDL
ISRLLQVDPEARLTAEQALQHPFFERCEGSQPWN*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU1937
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial construct to express GST N terminal fusion