cDNA Clone ERK5

• Synonyms: MAPK7
• Plasmid: pEGFP-C1 ERK5
• Parent Plasmid: pEGFPC1
• Expressed: GFP ERK5
• Genbank: NM_002749
• Tag: GFP
• Species: Human

Sequence of Insert

GGATCCGCCGAGCCTCTGAAGGAGGAAGACGGCGAGGACGGCTCTGCGGA
GCCCCCCGGGCCCGTGAAGGCCGAACCCGCCCACACCGCTGCCTCTGTAG
CGGCCAAGAACCTGGCCCTGCTTAAAGCCCGCTCCTTCGATGTGACCTTT
GACGTGGGCGACGAGTACGAGATCATCGAGACCATAGGCAACGGGGCCTA
TGGAGTGGTGTCCTCCGCCCGCCGCCGCCTCACCGGCCAGCAGGTGGCCA
TCAAGAAGATCCCTAATGCTTTCGATGTGGTGACCAATGCCAAGCGGACC
CTCAGGGAGCTGAAGATCCTCAAGCACTTTAAACACGACAACATCATCGC
CATCAAGGACATCCTGAGGCCCACCGTGCCCTATGGCGAATTCAAATCTG
TCTACGTGGTCCTGGACCTGATGGAAAGCGACCTGCACCAGATCATCCAC
TCCTCACAGCCCCTCACACTGGAACACGTGCGCTACTTCCTGTACCAACT
GCTGCGGGGCCTGAAGTACATGCACTCGGCTCAGGTCATCCACCGTGACC
TGAAGCCCTCCAACCTATTGGTGAATGAGAACTGTGAGCTCAAGATTGGT
GACTTTGGTATGGCTCGTGGCCTGTGCACCTCGCCCGCTGAACATCAGTA
CTTCATGACTGAGTATGTGGCCACGCGCTGGTACCGTGCGCCCGAGCTCA
TGCTCTCTTTGCATGAGTATACACAGGCTATTGACCTCTGGTCTGTGGGC
TGCATCTTTGGTGAGATGCTGGCCCGGCGCCAGCTCTTCCCAGGCAAAAA
CTATGTACACCAGCTACAGCTCATCATGATGGTGCTGGGTACCCCATCAC
CAGCCGTGATTCAGGCTGTGGGGGCTGAGAGGGTGCGGGCCTATATCCAG
AGCTTGCCACCACGCCAGCCTGTGCCCTGGGAGACAGTGTACCCAGGTGC
CGACCGCCAGGCCCTATCACTGCTGGGTCGCATGCTGCGTTTTGAGCCCA
GCGCTCGCATCTCAGCAGCTGCTGCCCTTCGCCACCCTTTCCTGGCCAAG
TACCATGATCCTGATGATGAGCCTGACTGTGCCCCGCCCTTTGACTTTGC
CTTTGACCGCGAAGCCCTCACTCGGGAGCGCATTAAGGAGGCCATTGTGG
CTGAAATTGAGGACTTCCATGCAAGGCGTGAGGGCATCCGCCAACAGATC
CGCTTCCAGCCTTCTCTACAGCCTGTGGCTAGTGAGCCTGGCTGTCCAGA
TGTTGAAATGCCCAGTCCCTGGGCTCCCAGTGGGGACTGTGCCATGGAGT
CTCCACCACCAGCCCCGCCACCATGCCCCGGCCCTGCACCTGACACCATT
GATCTGACCCTGCAGCCACCTCCACCAGTCAGTGAGCCTGCCCCACCAAA
GAAAGATGGTGCCATCTCAGACAATACTAAGGCTGCCCTTAAAGCTGCCC
TGCTCAAGTCTTTGAGGAGCCGGCTCAGAGATGGCCCCAGCGCACCCCTG
GAGGCTCCTGAGCCTCGGAAGCCGGTGACAGCCCAGGAGCGCCAGCGGGA
GCGGGAGGAGAAGCGGCGGAGGCGGCAAGAACGAGCCAAGGAGCGGGAGA
AACGGCGGCAGGAGCGGGAGCGAAAGGAACGGGGGGCTGGGGCCTCTGGG
GGCCCCTCCACTGACCCCTTGGCTGGACTAGTGCTCAGTGACAATGACAG
AAGCCTGTTGGAACGCTGGACTCGAATGGCCCGGCCCGCAGCCCCAGCCC
TCACCTCTGTGCCGGCCCCTGCCCCAGCGCCAACGCCAACCCCAACCCCA
GTCCAACCTACCAGTCCTCCTCCTGGCCCTGTAGCCCAGCCCACTGGCCC
GCAACCACAATCTGCGGGCTCTACCTCTGGCCCTGTACCCCAGCCTGCCT
GCCCACCCCCTGGCCCTGCACCCCACCCCACTGGCCCTCCTGGGCCCATC
CCTGTCCCCGCGCCACCCCAGATTGCCACCTCCACCAGCCTCCTGGCTGC
CCAGTCACTTGTGCCACCCCCTGGGCTGCCTGGCTCCAGCACCCCAGGAG
TTTTGCCTTACTTCCCACCTGGCCTGCCGCCCCCAGACGCCGGGGGAGCC
CCTCAGTCTTCCATGTCAGAGTCACCTGATGTCAACCTTGTGACCCAGCA
GCTATCTAAGTCACAGGTGGAGGACCCCCTGCCCCCTGTGTTCTCAGGCA
CACCAAAGGGCAGTGGGGCTGGCTACGGTGTTGGCTTTGACCTGGAGGAA
TTCTTAAACCAGTCTTTCGACATGGGCGTGGCTGATGGGCCACAGGATGG
CCAGGCAGATTCAGCCTCTCTCTCAGCCTCCCTGCTTGCTGACTGGCTCG
AAGGCCATGGCATGAACCCTGCCGATATTGAGTCCCTGCAGCGTGAGATC
CAGATGGACTCCCCAATGCTGCTGGCTGACCTGCCTGACCTCCAGGACCC
CTGAGGATCC

Amino Acid Sequence

MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICT
TGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIF
FKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHN
VYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
YLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKSGLRSRAQASN
SAVDGTAGPGSAEPLKEEDGEDGSAEPPGPVKAEPAHTAASVAAKNLALL
KARSFDVTFDVGDEYEIIETIGNGAYGVVSSARRRLTGQQVAIKKIPNAF
DVVTNAKRTLRELKILKHFKHDNIIAIKDILRPTVPYGEFKSVYVVLDLM
ESDLHQIIHSSQPLTLEHVRYFLYQLLRGLKYMHSAQVIHRDLKPSNLLV
NENCELKIGDFGMARGLCTSPAEHQYFMTEYVATRWYRAPELMLSLHEYT
QAIDLWSVGCIFGEMLARRQLFPGKNYVHQLQLIMMVLGTPSPAVIQAVG
AERVRAYIQSLPPRQPVPWETVYPGADRQALSLLGRMLRFEPSARISAAA
ALRHPFLAKYHDPDDEPDCAPPFDFAFDREALTRERIKEAIVAEIEDFHA
RREGIRQQIRFQPSLQPVASEPGCPDVEMPSPWAPSGDCAMESPPPAPPP
CPGPAPDTIDLTLQPPPPVSEPAPPKKDGAISDNTKAALKAALLKSLRSR
LRDGPSAPLEAPEPRKPVTAQERQREREEKRRRRQERAKEREKRRQERER
KERGAGASGGPSTDPLAGLVLSDNDRSLLERWTRMARPAAPALTSVPAPA
PAPTPTPTPVQPTSPPPGPVAQPTGPQPQSAGSTSGPVPQPACPPPGPAP
HPTGPPGPIPVPAPPQIATSTSLLAAQSLVPPPGLPGSSTPGVLPYFPPG
LPPPDAGGAPQSSMSESPDVNLVTQQLSKSQVEDPLPPVFSGTPKGSGAG
YGVGFDLEEFLNQSFDMGVADGPQDGQADSASLSASLLADWLEGHGMNPA
DIESLQREIQMDSPMLLADLPDLQDP*

• Vector Type: Mammalian
• Antibiotic: Kan
• Full Snapgene Map: pEGFP-C1 ERK5226.04 KB Snapgene files can be opened using software available here: www.snapgene.com
• DU Number: DU1911
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Mammalian N terminal GFP.
• Low Temp: No