cDNA Clone EWS

• Plasmid: pGEX6P-1-EWS
• Parent Plasmid: pGEX6P1
• Expressed: GST-EWS
• Genbank: NP_005234
• Tag: GST
• Species: Human

Sequence of Insert

GAATTCGCGTCCACGGATTACAGTACCTATAGCCAAGCTGCAGCGCAGCA
GGGCTACAGTGCTTACACCGCCCAGCCCACTCAAGGATATGCACAGACCA
CCCAGGCATATGGGCAACAAAGCTATGGAACCTATGGACAGCCCACTGAT
GTCAGCTATACCCAGGCTCAGACCACTGCAACCTATGGGCAGACCGCCTA
TGCAACTTCTTATGGACAGCCTCCCACTGGTTATACTACTCCAACTGCCC
CCCAGGCATACAGCCAGCCTGTCCAGGGGTATGGCACTGGTGCTTATGAT
ACCACCACTGCTACAGTCACCACCACCCAGGCCTCCTATGCAGCTCAGTC
TGCATATGGCACTCAGCCTGCTTATCCAGCCTATGGGCAGCAGCCAGCAG
CCACTGCACCTACAAGACCGCAGGATGGAAACAAGCCCACTGAGACTAGT
CAACCTCAATCTAGCACAGGGGGTTACAACCAGCCCAGCCTAGGATATGG
ACAGAGTAACTACAGTTATCCCCAGGTACCTGGGAGCTACCCCATGCAGC
CAGTCACTGCACCTCCATCCTACCCTCCTACCAGCTATTCCTCTACACAG
CCGACTAGTTATGATCAGAGCAGTTACTCTCAGCAGAACACCTATGGGCA
ACCGAGCAGCTATGGACAGCAGAGTAGCTATGGTCAACAAAGCAGCTATG
GGCAGCAGCCTCCCACTAGTTACCCACCCCAAACTGGATCCTACAGCCAA
GCTCCAAGTCAATATAGCCAACAGAGCAGCAGCTACGGGCAGCAGAGTTC
ATTCCGACAGGACCACCCCAGTAGCATGGGTGTTTATGGGCAGGAGTCTG
GAGGATTTTCCGGACCAGGAGAGAACCGGAGCATGAGTGGCCCTGATAAC
CGGGGCAGGGGAAGAGGGGGATTTGATCGTGGAGGCATGAGCAGAGGTGG
GCGGGGAGGAGGACGCGGTGGAATGGGCAGCGCTGGAGAGCGAGGTGGCT
TCAATAAGCCTGGTGGACCCATGGATGAAGGACCAGATCTTGATCTAGGC
CCTCCTGTAGATCCAGATGAAGACTCTGACAACAGTGCAATTTATGTACA
AGGATTAAATGACAGTGTGACTCTAGATGATCTGGCAGACTTCTTTAAGC
AGTGTGGGGTTGTTAAGATGAACAAGAGAACTGGGCAACCCATGATCCAC
ATCTACCTGGACAAGGAAACAGGAAAGCCCAAAGGCGATGCCACAGTGTC
CTATGAAGACCCACCCACTGCCAAGGCTGCCGTGGAATGGTTTGATGGGA
AAGATTTTCAAGGGAGCAAACTTAAAGTCTCCCTTGCTCGGAAGAAGCCT
CCAATGAACAGTATGCGGGGTGGTCTGCCACCCCGTGAGGGCAGAGGCAT
GCCACCACCACTCCGTGGAGGTCCAGGAGGCCCAGGAGGTCCTGGGGGAC
CCATGGGTCGCATGGGAGGCCGTGGAGGAGATAGAGGAGGCTTCCCTCCA
AGAGGACCCCGGGGTTCCCGAGGGAACCCCTCTGGAGGAGGAAACGTCCA
GCACCGAGCTGGAGACTGGCAGTGTCCCAATCCGGGTTGTGGAAACCAGA
ACTTCGCCTGGAGAACAGAGTGCAACCAGTGTAAGGCCCCAAAGCCTGAA
GGCTTCCTCCCGCCACCCTTTCCGCCCCCGGGTGGTGATCGTGGCAGAGG
TGGCCCTGGTGGCATGCGGGGAGGAAGAGGTGGCCTCATGGATCGTGGTG
GTCCCGGTGGAATGTTCAGAGGTGGCCGTGGTGGAGACAGAGGTGGCTTC
CGTGGTGGCCGGGGCATGGACCGAGGTGGCTTTGGTGGAGGAAGACGAGG
TGGCCCTGGGGGGCCCCCTGGACCTTTGATGGAACAGATGGGAGGAAGAA
GAGGAGGACGTGGAGGACCTGGAAAAATGGATAAAGGCGAGCACCGTCAG
GAGCGCAGAGATCGGCCCTACTAGGTCGAC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSPEFASTDYSTYSQAAAQQG
YSAYTAQPTQGYAQTTQAYGQQSYGTYGQPTDVSYTQAQTTATYGQTAYA
TSYGQPPTGYTTPTAPQAYSQPVQGYGTGAYDTTTATVTTTQASYAAQSA
YGTQPAYPAYGQQPAATAPTRPQDGNKPTETSQPQSSTGGYNQPSLGYGQ
SNYSYPQVPGSYPMQPVTAPPSYPPTSYSSTQPTSYDQSSYSQQNTYGQP
SSYGQQSSYGQQSSYGQQPPTSYPPQTGSYSQAPSQYSQQSSSYGQQSSF
RQDHPSSMGVYGQESGGFSGPGENRSMSGPDNRGRGRGGFDRGGMSRGGR
GGGRGGMGSAGERGGFNKPGGPMDEGPDLDLGPPVDPDEDSDNSAIYVQG
LNDSVTLDDLADFFKQCGVVKMNKRTGQPMIHIYLDKETGKPKGDATVSY
EDPPTAKAAVEWFDGKDFQGSKLKVSLARKKPPMNSMRGGLPPREGRGMP
PPLRGGPGGPGGPGGPMGRMGGRGGDRGGFPPRGPRGSRGNPSGGGNVQH
RAGDWQCPNPGCGNQNFAWRTECNQCKAPKPEGFLPPPFPPPGGDRGRGG
PGGMRGGRGGLMDRGGPGGMFRGGRGGDRGGFRGGRGMDRGGFGGGRRGG
PGGPPGPLMEQMGGRRGGRGGPGKMDKGEHRQERRDRPY*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU1883
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N terminal GST for bacterial expression