cDNA Clone BTK

• Synonyms: AT; ATK; BPK; XLA; IMD1; AGMX1; IGHD3; PSCTK1
• Plasmid: pEBG2T BTK
• Parent Plasmid: pEBG2T
• Expressed: GST Thrombin BTK
• Genbank: NM_000061
• Tag: GST
• Species: Human

Sequence of Insert

GGATCCGCCGCAGTGATTCTGGAGAGCATCTTTCTGAAGCGATCCCAACA
GAAAAAGAAAACATCACCTCTAAACTTCAAGAAGCGCCTGTTTCTCTTGA
CCGTGCACAAACTCTCCTACTATGAGTATGACTTTGAACGTGGGAGAAGA
GGCAGTAAGAAGGGTTCAATAGATGTTGAGAAGATCACTTGTGTTGAAAC
AGTGGTTCCTGAAAAAAATCCTCCTCCAGAAAGACAGATTCCGAGAAGAG
GTGAAGAGTCCAGTGAAATGGAGCAAATTTCAATCATTGAAAGGTTCCCT
TATCCCTTCCAGGTTGTATATGATGAAGGGCCTCTCTACGTCTTCTCCCC
AACTGAAGAACTAAGGAAGCGGTGGATTCACCAGCTCAAAAACGTAATCC
GGTACAACAGTGATCTGGTTCAGAAATATCACCCTTGCTTCTGGATCGAT
GGGCAGTATCTCTGCTGCTCTCAGACAGCCAAAAATGCTATGGGCTGCCA
AATTTTGGAGAACAGGAATGGAAGCTTAAAACCTGGGAGTTCTCACCGGA
AGACAAAAAAGCCTCTTCCCCCAACGCCTGAGGAGGACCAGATCTTGAAA
AAGCCACTACCGCCTGAGCCAGCAGCAGCACCAGTCTCCACAAGTGAGCT
GAAAAAGGTTGTGGCCCTTTATGATTACATGCCAATGAATGCAAATGATC
TACAGCTGCGGAAGGGTGATGAATATTTTATCTTGGAGGAAAGCAACTTA
CCATGGTGGAGAGCACGAGATAAAAATGGGCAGGAAGGCTACATTCCTAG
TAACTATGTCACTGAAGCAGAAGACTCCATAGAAATGTATGAGTGGTATT
CCAAACACATGACTCGGAGTCAGGCTGAGCAACTGCTAAAGCAAGAGGGG
AAAGAAGGAGGTTTCATTGTCAGAGACTCCAGCAAAGCTGGCAAATATAC
AGTGTCTGTGTTTGCTAAATCCACAGGGGACCCTCAAGGGGTGATACGTC
ATTATGTTGTGTGTTCCACACCTCAGAGCCAGTATTACCTGGCTGAGAAG
CACCTTTTCAGCACCATCCCTGAGCTCATTAACTACCATCAGCACAACTC
TGCAGGACTCATATCCAGGCTCAAATATCCAGTGTCTCAACAAAACAAGA
ATGCACCTTCCACTGCAGGCCTGGGATACGGATCATGGGAAATTGATCCA
AAGGACCTGACCTTCTTGAAGGAGCTGGGGACTGGACAATTTGGGGTAGT
GAAGTATGGGAAATGGAGAGGCCAGTACGACGTGGCCATCAAGATGATCA
AAGAAGGCTCCATGTCTGAAGATGAATTCATTGAAGAAGCCAAAGTCATG
ATGAATCTTTCCCATGAGAAGCTGGTGCAGTTGTATGGCGTCTGCACCAA
GCAGCGCCCCATCTTCATCATCACTGAGTACATGGCCAATGGCTGCCTCC
TGAACTACCTGAGGGAGATGCGCCACCGCTTCCAGACTCAGCAGCTGCTA
GAGATGTGCAAGGATGTCTGTGAAGCCATGGAATACCTGGAGTCAAAGCA
GTTCCTTCACCGAGACCTGGCAGCTCGAAACTGTTTGGTAAACGATCAAG
GAGTTGTTAAAGTATCTGATTTCGGCCTGTCCAGGTATGTCCTGGATGAT
GAATACACAAGCTCAGTAGGCTCCAAATTTCCAGTCCGGTGGTCCCCACC
GGAAGTCCTGATGTATAGCAAGTTCAGCAGCAAATCTGACATTTGGGCTT
TTGGGGTTTTGATGTGGGAAATTTACTCCCTGGGGAAGATGCCATATGAG
AGATTTACTAACAGTGAGACTGCTGAACACATTGCCCAAGGCCTACGTCT
CTACAGGCCTCATCTGGCTTCAGAGAAGGTATATACCATCATGTACAGTT
GCTGGCATGAGAAAGCAGATGAGCGTCCCACTTTCAAAATTCTTCTGAGC
AATATTCTAGATGTCATGGATGAAGAATCCTGAGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLVPRGSAAVILESIFLKRSQQKKKTSPLNF
KKRLFLLTVHKLSYYEYDFERGRRGSKKGSIDVEKITCVETVVPEKNPPP
ERQIPRRGEESSEMEQISIIERFPYPFQVVYDEGPLYVFSPTEELRKRWI
HQLKNVIRYNSDLVQKYHPCFWIDGQYLCCSQTAKNAMGCQILENRNGSL
KPGSSHRKTKKPLPPTPEEDQILKKPLPPEPAAAPVSTSELKKVVALYDY
MPMNANDLQLRKGDEYFILEESNLPWWRARDKNGQEGYIPSNYVTEAEDS
IEMYEWYSKHMTRSQAEQLLKQEGKEGGFIVRDSSKAGKYTVSVFAKSTG
DPQGVIRHYVVCSTPQSQYYLAEKHLFSTIPELINYHQHNSAGLISRLKY
PVSQQNKNAPSTAGLGYGSWEIDPKDLTFLKELGTGQFGVVKYGKWRGQY
DVAIKMIKEGSMSEDEFIEEAKVMMNLSHEKLVQLYGVCTKQRPIFIITE
YMANGCLLNYLREMRHRFQTQQLLEMCKDVCEAMEYLESKQFLHRDLAAR
NCLVNDQGVVKVSDFGLSRYVLDDEYTSSVGSKFPVRWSPPEVLMYSKFS
SKSDIWAFGVLMWEIYSLGKMPYERFTNSETAEHIAQGLRLYRPHLASEK
VYTIMYSCWHEKADERPTFKILLSNILDVMDEES*

• Vector Type: Mammalian
• Antibiotic: Amp
• Full Snapgene Map: pEBG2T BTK599.39 KBSnapgene files can be opened using software available here: www.snapgene.com
• DU Number: DU1855
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N terminal GST tag for mammalian expression
• Low Temp: No