cDNA Clone PRAK

• Synonyms: MAPKAPK5
• Plasmid: pFB PRAK
• Parent Plasmid: pFastbac1
• Expressed: 6His-PRAK
• Genbank: AAH41049
• Tag: 6His
• Species: Human

Sequence of Insert

GGATCCACCATGCACCATCACCATCACCATATGTCGGAGGAGAGCGACAT
GGACAAAGCCATCAAGGAAACTTCCATTTTAGAAGAATACAGTATCAATT
GGACTCAGAAGCTGGGAGCTGGAATTAGTGGTCCAGTTAGAGTCTGTGTA
AAGAAATCTACTCAAGAACGGTTTGCGCTGAAAATTCTTCTTGATCGTCC
AAAAGCTAGAAATGAGGTACGTCTGCACATGATGTGTGCCACACACCCAA
ACATAGTTCAGATTATTGAAGTGTTTGCTAACAGTGTCCAGTTTCCCCAT
GAGTCCAGCCCTAGGGCCCGACTCTTAATTGTAATGGAGATGATGGAAGG
GGGAGAGCTATTTCACAGAATCAGCCAGCACCGGCACTTTACAGAGAAGC
AAGCCAGCCAAGTAACAAAGCAGATAGCTTTGGCTCTGCGGCACTGTCAC
TTGTTAAACATTGCGCACAGAGACCTCAAGCCTGAAAATCTGCTTTTTAA
GGATAACTCTTTGGATGCCCCAGTGAAGTTGTGTGACTTTGGATTTGCCA
AGATTGACCAAGGTGACTTGATGACACCCCAGTTCACCCCTTATTATGTA
GCACCCCAGGTACTGGAGGCGCAAAGAAGGCATCAGAAGGAGAAATCTGG
CATCATACCTACCTCACCGACGCCCTACACTTACAACAAGAGCTGTGACT
TGTGGTCCCTAGGGGTGATTATCTATGTGATGCTGTGCGGATACCCTCCT
TTTTACTCCAAACACCACAGCCGGACTATCCCAAAGGATATGCGAAGAAA
GATCATGACAGGCAGTTTTGAGTTCCCAGAGGAAGAGTGGAGTCAGATCT
CAGAGATGGCCAAAGATGTTGTGAGGAAGCTCCTGAAGGTCAAACCGGAG
GAGAGACTCACCATCGAGGGAGTGCTGGACCACCCCTGGCTCAATTCCAC
CGAGGCCCTGGATAATGTGCTGCCTTCTGCTCAGCTGATGATGGACAAGG
CAGTGGTTGCAGGAATCCAGCAGGCTCACGCGGAACAGTTGGCCAACATG
AGAATCCAGGATCTGAAAGTCAGCCTCAAACCCCTGCACTCAGTGAACAA
CCCCATTCTGCGGAAGAGGAAGTTACTTGGCACCAAGCCAAAGGACAGTG
TCTATATCCACGACCATGAGAATGGAGCCGAGGATTCCAATGTTGCCTTG
GAAAAACTCCGAGATGTGATTGCTCAGTGTATTCTCCCCCAGGCTGGAGA
GAATGAAGATGAGAAACTGAATGAAGTAATGCAGGAGGCTTGGAAGTATA
ACCGGGAATGCAAACTCCTAAGAGATACTCTGCAGAGCTTCAGCTGGAAT
GGTCGTGGATTCACAGATAAAGTAGATCGACTAAAACTGGCAGAAATTGT
GAAGCAGGTGATAGAAGAGCAAACCACGTCCCACGAATCCCAATAACTCG
AG

Amino Acid Sequence

MHHHHHHMSEESDMDKAIKETSILEEYSINWTQKLGAGISGPVRVCVKKS
TQERFALKILLDRPKARNEVRLHMMCATHPNIVQIIEVFANSVQFPHESS
PRARLLIVMEMMEGGELFHRISQHRHFTEKQASQVTKQIALALRHCHLLN
IAHRDLKPENLLFKDNSLDAPVKLCDFGFAKIDQGDLMTPQFTPYYVAPQ
VLEAQRRHQKEKSGIIPTSPTPYTYNKSCDLWSLGVIIYVMLCGYPPFYS
KHHSRTIPKDMRRKIMTGSFEFPEEEWSQISEMAKDVVRKLLKVKPEERL
TIEGVLDHPWLNSTEALDNVLPSAQLMMDKAVVAGIQQAHAEQLANMRIQ
DLKVSLKPLHSVNNPILRKRKLLGTKPKDSVYIHDHENGAEDSNVALEKL
RDVIAQCILPQAGENEDEKLNEVMQEAWKYNRECKLLRDTLQSFSWNGRG
FTDKVDRLKLAEIVKQVIEEQTTSHESQ*

• Vector Type: Baculovirus
• Antibiotic: Amp
• DU Number: DU1853
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N ter 6His for baculovirus expression.; Estimated MW of expressed protein : 54987 Daltons