cDNA Clone MEKK1 (mouse)

• Synonyms: Map3k1
• Plasmid: pET MEKK1 6His
• Parent Plasmid: pET21a original
• Expressed: MEKK1 6His
• Genbank: L13103
• Tag: 6His
• Species: Mouse

Sequence of Insert

CATATGGTGACCGCAGTGCCCGCTGTGTTTTCCAAGCTGGTAACCATGCT
TAATGCTTCTGGCTCCACCCACTTCACCAGGATGCGCCGGCGTCTGATGG
CTATCGCGGATGAGGTAGAAATTGCCGAGGTCATCCAGCTGGGTGTGGAG
GACACTGTGGATGGGCATCAGGACAGCTTACAGGCGCTGGCCCCCGCCAG
CTGTCTAGAAAACAGCTCCCTTGAGCACACAGTCCATAGAGAGAAAACTG
GAAAAGGACTAAGTGCTACGAGACTGAGTGCCAGCTCGGAGGACATTTCT
GACAGACTGGCCGGCGTCTCTGTAGGACTTCCCAGCTCAACAACAACAGA
ACAACCAAAGCCAGCGGTTCAAACAAAAGGCAGACCCCACAGTCAGTGTT
TGAACTCCTCCCCTTTGTCTCATGCTCAATTAATGTTCCCAGCACCATCA
GCCCCTTGTTCCTCTGCCCCGTCTGTCCCAGATATTTCTAAGCACAGACC
CCAGGCATTTGTTCCCTGCAAAATACCTTCCGCATCTCCTCAGACACAGC
GCAAGTTCTCTCTACAATTCCAGAGGAACTGCTCTGAACACCGAGACTCA
GACCAGCTCTCCCCAGTCTTCACTCAGTCAAGACCCCCACCCTCCAGTAA
CATACACAGGCCAAAGCCATCCCGACCCGTTCCGGGCAGTACAAGCAAAC
TAGGGGACGCCACAAAAAGTAGCATGACACTTGATCTGGGCAGTGCTTCC
AGGTGTGACGACAGCTTTGGCGGCGGCGGCAACAGTGGCAACGCCGTCAT
ACCCAGCGACGAGACAGTGTTCACGCCGGTGGAGGACAAGTGCAGGTTAG
ATGTGAACACCGAGCTCAACTCCAGCATCGAGGACCTTCTTGAAGCATCC
ATGCCTTCAAGTGACACGACAGTCACTTTCAAGTCCGAAGTCGCCGTCCT
CTCTCCGGAAAAGGCCGAAAATGACGACACCTACAAAGACGACGTCAATC
ATAATCAAAAGTGCAAAGAAAAGATGGAAGCTGAAGAGGAGGAGGCTTTA
GCGATCGCCATGGCGATGTCAGCGTCTCAGGATGCCCTCCCCATCGTCCC
TCAGCTGCAGGTGGAAAATGGAGAAGATATTATCATCATTCAGCAGGACA
CACCAGAAACTCTTCCAGGACATACCAAAGCGAAACAGCCTTACAGAGAA
GACGCTGAGTGGCTGAAAGGCCAGCAGATAGGCCTCGGAGCATTTTCTTC
CTGTTACCAAGCACAGGATGTGGGGACTGGGACTTTAATGGCTGTGAAAC
AGGTGACGTACGTCAGAAACACATCCTCCGAGCAGGAGGAGGTGGTGGAA
GCGTTGAGGGAAGAGATCCGGATGATGGGTCACCTCAACCATCCAAACAT
CATCCGGATGCTGGGGGCCACGTGCGAGAAGAGCAACTACAACCTCTTCA
TTGAGTGGATGGCGGGAGGATCTGTGGCTCACCTCTTGAGTAAATACGGA
GCTTTCAAGGAGTCAGTCGTCATTAACTACACTGAGCAGTTACTGCGTGG
CCTTTCCTATCTCCACGAGAACCAGATCATTCACAGAGACGTCAAAGGTG
CCAACCTGCTCATTGACAGCACCGGTCAGAGGCTGAGAATTGCAGACTTT
GGAGCTGCTGCCAGGTTGGCATCAAAAGGAACCGGTGCAGGAGAGTTCCA
GGGACAGTTACTGGGGACAATTGCATTCATGGCGCCTGAGGTCCTAAGAG
GTCAGCAGTATGGTAGGAGCTGTGATGTATGGAGTGTTGGCTGCGCCATT
ATAGAAATGGCTTGTGCAAAACCACCTTGGAATGCAGAAAAACACTCCAA
TCATCTCGCCTTGATATTTAAGATTGCTAGCGCAACTACTGCACCGTCCA
TCCCGTCACACCTGTCCCCGGGTCTGCGCGACGTGGCGCTGCGCTGCTTA
GAACTTCAGCCTCAGGACCGGCCTCCGTCCAGAGAGCTGCTGAAACATCC
GGTCTTCCGTACCACGGATCC

Amino Acid Sequence

MVTAVPAVFSKLVTMLNASGSTHFTRMRRRLMAIADEVEIAEVIQLGVED
TVDGHQDSLQALAPASCLENSSLEHTVHREKTGKGLSATRLSASSEDISD
RLAGVSVGLPSSTTTEQPKPAVQTKGRPHSQCLNSSPLSHAQLMFPAPSA
PCSSAPSVPDISKHRPQAFVPCKIPSASPQTQRKFSLQFQRNCSEHRDSD
QLSPVFTQSRPPPSSNIHRPKPSRPVPGSTSKLGDATKSSMTLDLGSASR
CDDSFGGGGNSGNAVIPSDETVFTPVEDKCRLDVNTELNSSIEDLLEASM
PSSDTTVTFKSEVAVLSPEKAENDDTYKDDVNHNQKCKEKMEAEEEEALA
IAMAMSASQDALPIVPQLQVENGEDIIIIQQDTPETLPGHTKAKQPYRED
AEWLKGQQIGLGAFSSCYQAQDVGTGTLMAVKQVTYVRNTSSEQEEVVEA
LREEIRMMGHLNHPNIIRMLGATCEKSNYNLFIEWMAGGSVAHLLSKYGA
FKESVVINYTEQLLRGLSYLHENQIIHRDVKGANLLIDSTGQRLRIADFG
AAARLASKGTGAGEFQGQLLGTIAFMAPEVLRGQQYGRSCDVWSVGCAII
EMACAKPPWNAEKHSNHLALIFKIASATTAPSIPSHLSPGLRDVALRCLE
LQPQDRPPSRELLKHPVFRTTDPNSSSVDKLAAALEHHHHHH*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU1847
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: C terminal 6His for bacterial expression.