cDNA Clone UBE2V1

• Plasmid: pFBHTb UeV1a new
• Parent Plasmid: pFastBac HTb
• Expressed: 6His-UeV1a new
• Genbank: AY008273
• Tag: 6His
• Species: Human

Sequence of Insert

GGATCCCCAGGAGAGGTTCAAGCGTCTTACCTGAAGTCACAAAGCAAACT
GAGTGATGAAGGAAGACTTGAACCTAGAAAATTTCACTGCAAAGGAGTAA
AAGTCCCTCGCAATTTCCGACTGTTGGAAGAACTCGAAGAAGGCCAGAAA
GGAGTAGGAGATGGCACAGTTAGCTGGGGTCTAGAAGATGACGAAGACAT
GACACTTACAAGATGGACAGGGATGATAATTGGGCCTCCAAGAACAATTT
ATGAAAACCGAATATACAGCCTTAAAATAGAATGTGGACCTAAATACCCA
GAAGCACCCCCCTTTGTAAGATTTGTAACAAAAATTAATATGAATGGAGT
AAATAGTTCTAATGGAGTGGTGGACCCAAGAGCCATATCAGTGCTAGCAA
AATGGCAGAATTCATATAGCATCAAAGTTGTCCTGCAAGAGCTTCGGCGC
CTAATGATGTCTAAAGAAAATATGAAACTCCCTCAGCCGCCCGAAGGACA
GTGTTACAGCAATTAAGTCGAC

Amino Acid Sequence

MSYYHHHHHHDYDIPTTENLYFQGAMGSPGEVQASYLKSQSKLSDEGRLE
PRKFHCKGVKVPRNFRLLEELEEGQKGVGDGTVSWGLEDDEDMTLTRWTG
MIIGPPRTIYENRIYSLKIECGPKYPEAPPFVRFVTKINMNGVNSSNGVV
DPRAISVLAKWQNSYSIKVVLQELRRLMMSKENMKLPQPPEGQCYSN*

• Vector Type: Baculovirus
• Antibiotic: Amp
• DU Number: DU1833
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N terminal GST tag for baculovirus expression.