cDNA Clone UBE2V1

• Plasmid: pEBG-2T-UeV1a
• Parent Plasmid: pEBG2T
• Expressed: GST-UeV1a
• Genbank: NM_021988
• Tag: GST
• Species: Human

Sequence of Insert

GGATCCCCAGGAGAGGTTCAAGCGTCTTACCTGAAGTCACAAAGCAAACT
GAGTGATGAAGGAAGACTTGAACCTAGAAAATTTCACTGCAAAGGGAGTA
AAAGTCCCTCGCAATTTCGACTGTTGGAAGAACTCGAAGAAGGCCAGAAA
GGAGTAGGAGATGGCACAGTTAGCTGGGGTCTAGAAGATGACGAAGACAT
GACACTTACAAGATGGACAGGGATGATAATTGGGCCTCCAAGAACAATTT
ATGAAAACCGAATATACAGCCTTAAAATAGAATGTGGACCTAAATACCCA
GAAGCACCCCCCTTTGTAAGATTTGTAACAAAAATTAATATGAATGGAGT
AAATAGTTCTAATGGAGTGGTGGACCCAAGAGCCATATCAGTGCTAGCAA
AATGGCAGAATTCATATAGCATCAAAGTTGTCCTGCAAGAGCTTCGGCGC
CTAATGATGTCTAAAGAAAATATGAAACTCCCTCAGCCGCCCGAAGGACA
GTGTTACAGCAATTAAGTCGAC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLVPRGSPGEVQASYLKSQSKLSDEGRLEPR
KFHCKGSKSPSQFRLLEELEEGQKGVGDGTVSWGLEDDEDMTLTRWTGMI
IGPPRTIYENRIYSLKIECGPKYPEAPPFVRFVTKINMNGVNSSNGVVDP
RAISVLAKWQNSYSIKVVLQELRRLMMSKENMKLPQPPEGQCYSN*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU1830
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N terminal GST tag for Mammalian expression.