cDNA Clone METTL1

• Plasmid: pGEX6P-1-METTL1
• Parent Plasmid: pGEX6P1
• Expressed: GST-METTL1
• Genbank: Y18643
• Tag: GST
• Species: Human

Sequence of Insert

GGATCCGCAGCCGAGACTCGGAACGTGGCCGGAGCAGAGGCCCCACCGCC
CCAGAAGCGCTACTACCGGCAACGTGCTCACTCCAACCCCATGGCGGACC
ACACGCTGCGCTACCCTGTGAAGCCAGAGGAGATGGACTGGTCTGAGCTA
TACCCAGAGTTCTTCGCTCCACTCACTCAAAATCAGAGCCACGATGACCC
AAAGGATAAGAAAGAAAAGAGAGCTCAGGCCCAAGTGGAGTTTGCAGACA
TAGGCTGTGGCTATGGTGGCCTGTTAGTGGAACTGTCACCGCTGTTCCCA
GACACACTTATTCTGGGTCTGGAGATCCGGGTGAAGGTCTCAGACTATGT
ACAAGACCGGATTCGGGCCCTACGCGCAGCTCCTGCAGGTGGCTTCCAGA
ACATCGCCTGTCTCCGTAGCAATGCCATGAAGCACCTTCCTAACTTCTTC
TACAAGGGCCAGCTGACAAAGATGTTCTTCCTCTTCCCCGACCCACATTT
CAAGCGGACAAAGCACAAGTGGCGAATCATCAGTCCCACCCTGCTAGCAG
AATATGCCTACGTGCTAAGAGTTGGGGGGCTGGTGTATACCATAACCGAT
GTGCTGGAGCTACACGACTGGATGTGCACTCATTTCGAAGAGCACCCACT
GTTTGAGCGTGTGCCTCTGGAGGACCTGAGTGAAGACCCCGTTGTGGGAC
ATCTAGGCACCTCAACTGAGGAGGGGAAGAAAGTTCTACGTAATGGAGGG
AAGAATTTCCCAGCCATCTTCCGAAGAATACAAGATCCCGTCCTCCAGGC
AGTGACCTCCCAAACCAGCCTGCCTGGTCACTGAGGATCC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSAAETRNVAGAEAPPPQKRY
YRQRAHSNPMADHTLRYPVKPEEMDWSELYPEFFAPLTQNQSHDDPKDKK
EKRAQAQVEFADIGCGYGGLLVELSPLFPDTLILGLEIRVKVSDYVQDRI
RALRAAPAGGFQNIACLRSNAMKHLPNFFYKGQLTKMFFLFPDPHFKRTK
HKWRIISPTLLAEYAYVLRVGGLVYTITDVLELHDWMCTHFEEHPLFERV
PLEDLSEDPVVGHLGTSTEEGKKVLRNGGKNFPAIFRRIQDPVLQAVTSQ
TSLPGH*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU1806
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial construct to express GST N terminal fusion