cDNA Clone PKBa

• Synonyms: AKT1
• Plasmid: pGEX-PKBalpha 1-123
• Parent Plasmid: pGEX6P1
• Expressed: GST-PKBalpha 1-123
• Genbank: M63167
• Tag: GST
• Species: Human

Sequence of Insert

GGGATCCATGAGCGACGTGGCTATTGTGAAGGAGGGTTGGCTGCACAAAC
GAGGGGAGTACATCAAGACCTGGCGGCCACGCTACTTCCTCCTCAAGAAT
GATGGCACCTTCATTGGCTACAAGGAGCGGCCGCAGGATGTGGACCAACG
TGAGGCTCCCCTCAACAACTTCTCTGTGGCGCAGTGCCAGCTGATGAAGA
CGGAGCGGCCCCGGCCCAACACCTTCATCATCCGCTGCCTGCAGTGGACC
ACTGTCATCGAACGCACCTTCCATGTGGAGACTCCTGAGGAGCGGGAGGA
GTGGACAACCGCCATCCAGACTGTGGCTGACGGCCTCAAGAAGCAGGAGG
AGGAGGAGATGGACTTCCGGTCGGGCTGAGGATCC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMSDVAIVKEGWLHKRGEYI
KTWRPRYFLLKNDGTFIGYKERPQDVDQREAPLNNFSVAQCQLMKTERPR
PNTFIIRCLQWTTVIERTFHVETPEEREEWTTAIQTVADGLKKQEEEEMD
FRSG*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU1789
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N terminal GST for bacterial expression.