cDNA Clone Axin

• Plasmid: pGEX-Axin 237-468
• Parent Plasmid: pGEX4T1
• Expressed: GST-Axin 237-468
• Genbank: NP_003493
• Tag: GST
• Species: Human

Sequence of Insert

GAATTCGCCCTTACCTGCCGACCTTAAATGAAGATGAGGAATGGAAGTGT
GACCAGGACATGGATGAGGACGATGGCAGAGACGCTGCTCCCCCCGGAAG
ACTCCCTCAGAAGCTGCTCCTGGAGACAGCTGCCCCGAGGGTCTCCTCCA
GTAGACGGTACAGCGAAGGCAGAGAGTTCAGGTATGGATCCTGGCGGGAG
CCAGTCAACCCCTATTATGTCAATGCCGGCTATGCCCTGGCCCCAGCCAC
CAGTGCCAACGACAGCGAGCAGCAGAGCCTGTCCAGCGATGCAGACACCC
TGTCCCTCACGGACAGCAGCGTGGATGGGATCCCCCCATACAGGATCCGT
AAGCAGCACCGCAGGGAGATGCAGGAGAGCGTGCAGGTCAATGGGCGGGT
GCCCCTACCTCACATTCCCCGCACGTACCGGGTGCCGAAGGAGGTCCGCG
TGGAGCCTCAGAAGTTCGCGGAGGAGCTCATCCACCGCCTGGAGGCTGTG
CAGCGCACGCGGGAGGCCGAGGAGAAGCTGGAGGAGCGGCTGAAGCGCGT
GCGCATGGAGGAGGAAGGTGAGGACGGCGATCCATCGTCAGGGCCCCCAG
GGCCGTGTCACAAGCTGCCTCCCGCCCCCGCTTGGCACCACTTCCCGCCC
CGCTGTGTGGACATGGGCTGTGCCGGGCTCCGGGATGCACACGAGGAGAA
CCCTGAGGAAGGGCGAATTC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLVPRGSPNPYLPTLNEDEEWKCDQDMDEDD
GRDAAPPGRLPQKLLLETAAPRVSSSRRYSEGREFRYGSWREPVNPYYVN
AGYALAPATSANDSEQQSLSSDADTLSLTDSSVDGIPPYRIRKQHRREMQ
ESVQVNGRVPLPHIPRTYRVPKEVRVEPQKFAEELIHRLEAVQRTREAEE
KLEERLKRVRMEEEGEDGDPSSGPPGPCHKLPPAPAWHHFPPRCVDMGCA
GLRDAHEENPEEGRIPGSTRAAAS*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU1786
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N terminal GST for bacterial expression.; Estimated MW of expressed protein : 53254 Daltons