cDNA Clone TPL2

• Synonyms: MAP3K8, COT, EST, ESTF, FLJ10486, MEKK8, TPL2, Tpl-2, c-COT, OTTHUMP00000019392, OTTHUMP00000019393, Ewing sarcoma transformant, cot (cancer Osaka thyroid) oncogene, proto-oncogene c-Cot, proto-oncogene serine/threoine protein kinase, tumor progression locus 2
• Plasmid: pFBHTb TPL2 New
• Parent Plasmid: pFastBac HTb
• Expressed: 6His-TPL2
• Genbank: P41279
• Tag: 6His
• Species: Human

Sequence of Insert

GGATCCGAGTACATGAGCACTGGAAGTGACAATAAAGAAGAGATTGATTT
ATTAATTAAACATTTAAATGTGTCTGATGTAATAGACATTATGGAAAATC
TTTATGCAAGTGAAGAGCCAGCAGTTTATGAACCCAGTCTAATGACCATG
TGTCAAGACAGTAATCAAAACGATGAGCGTTCTAAGTCTCTGCTGCTTAG
TGGCCAAGAGGTACCATGGTTGTCATCAGTCAGATATGGAACTGTGGAGG
ATTTGCTTGCTTTTGCAAACCATATATCCAACACTGCAAAGCATTTTTAT
GGACAACGACCACAGGAATCTGGAATTTTATTAAACATGGTCATCACTCC
CCAAAATGGACGTTACCAAATAGATTCCGATGTTCTCCTGATCCCCTGGA
AGCTGACTTACAGGAATATTGGTTCTGATTTTATTCCTCGGGGCGCCTTT
GGAAAGGTATACTTGGCACAAGATATAAAGACGAAGAAAAGAATGGCGTG
TAAACTGATCCCTGTAGATCAATTTAAGCCATCTGATGTGGAAATCCAGG
CTTGCTTCCGGCACGAGAACATCGCAGAGCTGTATGGCGCAGTCCTGTGG
GGTGAAACTGTCCATCTCTTTATGGAAGCAGGCGAGGGAGGGTCTGTTCT
GGAGAAACTGGAGAGCTGTGGACCAATGAGAGAATTTGAAATTATTTGGG
TGACAAAGCATGTTCTCAAGGGACTTGATTTTCTACACTCAAAGAAAGTG
ATCCATCATGATATTAAACCTAGCAACATTGTTTTCATGTCCACAAAAGC
TGTTTTGGTGGATTTTGGCCTAAGTGTTCAAATGACCGAAGATGTCTATT
TTCCTAAGGACCTCCGAGGAACAGAGATTTACATGAGCCCAGAGGTCATC
CTGTGCAGGGGCCATTCAACCAAAGCAGACATCTACAGCCTGGGGGCCAC
GCTCATCCACATGCAGACGGGCACCCCACCCTGGGTGAAGCGCTACCCTC
GCTCAGCCTATCCCTCCTACCTGTACATAATCCACAAGCAAGCACCTCCA
CTGGAAGACATTGCAGATGACTGCAGTCCAGGGATGAGAGAGCTGATAGA
AGCTTCCCTGGAGAGAAACCCCAATCACCGCCCAAGAGCCGCAGACCTAC
TAAAACATGAGGCCCTGAACCCGCCCAGAGAGGATCAGCCACGCTGTACG
AGTCTGGACTCTGCCCTCTTGGAGCGCAAGAGGCTGCTGAGTAGGAAGGA
GCTGGAACTTCCTGAGAACATTGCTGATTCTTCGTGCACAGGAAGCACCG
AGGAATCTGAGATGCTCAAGAGGCAACGCTCTCTCTACATCGACCTCGGC
GCTCTGGCCGGCTACTTCAATCTTGTTCGGGGACCACCAACGCTTGAATA
TGGCTGAGAATTC

Amino Acid Sequence

MSYYHHHHHHDYDIPTTENLYFQGAMGSEYMSTGSDNKEEIDLLIKHLNV
SDVIDIMENLYASEEPAVYEPSLMTMCQDSNQNDERSKSLLLSGQEVPWL
SSVRYGTVEDLLAFANHISNTAKHFYGQRPQESGILLNMVITPQNGRYQI
DSDVLLIPWKLTYRNIGSDFIPRGAFGKVYLAQDIKTKKRMACKLIPVDQ
FKPSDVEIQACFRHENIAELYGAVLWGETVHLFMEAGEGGSVLEKLESCG
PMREFEIIWVTKHVLKGLDFLHSKKVIHHDIKPSNIVFMSTKAVLVDFGL
SVQMTEDVYFPKDLRGTEIYMSPEVILCRGHSTKADIYSLGATLIHMQTG
TPPWVKRYPRSAYPSYLYIIHKQAPPLEDIADDCSPGMRELIEASLERNP
NHRPRAADLLKHEALNPPREDQPRCTSLDSALLERKRLLSRKELELPENI
ADSSCTGSTEESEMLKRQRSLYIDLGALAGYFNLVRGPPTLEYG*

• Vector Type: Baculovirus
• Antibiotic: Amp
• DU Number: DU1775
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Baculovirus expression plasmid with N terminal 6His tag