cDNA Clone UBE2V1

• Plasmid: pETBlue Precission UeV1a
• Parent Plasmid: pETBlue2 original
• Expressed: 6His UeV1a
• Genbank: NM_021988
• Tag: 6His
• Species: Human

Sequence of Insert

GGATCCCCACCATCACCATCACCATCTGGAAGTTCTGTTCCAGGGGCCCC
CAGGAGAGGTTCAAGCGTCTTACCTGAAGTCACAAAGCAAACTGAGTGAT
GAAGGAAGACTTGAACCTAGAAAATTTCACTGCAAAGGGAGTAAAAGTCC
CTCGCAATTTCGACTGTTGGAAGAACTCGAAGAAGGCCAGAAAGGAGTAG
GAGATGGCACAGTTAGCTGGGGTCTAGAAGATGACGAAGACATGACACTT
ACAAGATGGACAGGGATGATAATTGGGCCTCCAAGAACAATTTATGAAAA
CCGAATATACAGCCTTAAAATAGAATGTGGACCTAAATACCCAGAAGCAC
CCCCCTTTGTAAGATTTGTAACAAAAATTAATATGAATGGAGTAAATAGT
TCTAATGGAGTGGTGGACCCAAGAGCCATATCAGTGCTAGCAAAATGGCA
GAATTCATATAGCATCAAAGTTGTCCTGCAAGAGCTTCGGCGCCTAATGA
TGTCTAAAGAAAATATGAAACTCCCTCAGCCGCCCGAAGGACAGTGTTAC
AGCAATTAAGTCGAC

Amino Acid Sequence

MAISRELVDPHHHHHHLEVLFQGPPGEVQASYLKSQSKLSDEGRLEPRKF
HCKGSKSPSQFRLLEELEEGQKGVGDGTVSWGLEDDEDMTLTRWTGMIIG
PPRTIYENRIYSLKIECGPKYPEAPPFVRFVTKINMNGVNSSNGVVDPRA
ISVLAKWQNSYSIKVVLQELRRLMMSKENMKLPQPPEGQCYSN*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU1736
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N terminal 6His tag for bacterial expression.