cDNA Clone Axin

• Plasmid: pGEX6P-1-Axin 489-862 6His
• Parent Plasmid: pGEX6P1
• Expressed: GST-Axin 489-862 6His
• Genbank: NP_003493
• Tag: GST
• Species: Human

Sequence of Insert

GGATCCCCTGGCCATCGCTCCCCGGACAGTGGGCACGTGGCCAAGATGCC
AGTGGCACTGGGGGGTGCCGCCTCGGGGCACGGGAAGCACGTACCCAAGT
CAGGGGCGAAGCTGGACGCGGCCGGCCTGCACCACCACCGACACGTCCAC
CACCACGTCCACCACAGCACAGCCCGGCCCAAGGAGCAGGTGGAGGCCGA
GGCCACCCGCAGGGCCCAGAGCAGCTTCGCCTGGGGCCTGGAACCACACA
GCCATGGGGCAAGGTCCCGAGGCTACTCAGAGAGTGTTGGCGCTGCCCCC
AACGCCAGTGATGGCCTCGCCCACAGTGGGAAGGTGGGCGTGGCGTGCAA
AAGAAATGCCAAGAAGGCTGAGTCGGGGAAGAGCGCCAGCACCGAGGTGC
CAGGTGCCTCGGAGGATGCGGAGAAGAACCAGAAAATCATGCAGTGGATC
ATTGAGGGGGAAAAGGAGATCAGCAGGCACCGCAGGACCGGCCACGGGTC
TTCGGGGACGAGGAAGCCACAGCCCCATGAGAACTCCAGACCCTTGTCCC
TTGAGCACCCCTGGGCCGGCCCTCAGCTCCGGACCTCCGTGCAGCCCTCC
CACCTCTTCATCCAAGACCCCACCATGCCACCCCACCCAGCTCCCAACCC
CCTAACCCAGCTGGAGGAGGCGCGCCGACGTCTGGAGGAGGAAGAAAAGA
GAGCCAGCCGAGCACCCTCCAAGCAGAGGTATGTGCAGGAGGTTATGCGG
CGGGGACGCGCCTGCGTCAGGCCAGCGTGCGCGCCGGTGCTGCACGTGGT
ACCAGCCGTGTCGGACATGGAGCTCTCCGAGACAGAGACAAGATCGCAGA
GGAAGGTGGGCGGCGGGAGTGCCCAGCCGTGTGACAGCATCGTTGTGGCG
TACTACTTCTGCGGGGAACCCATCCCCTACCGCACCCTGGTGAGGGGCCG
CGCTGTCACCCTGGGCCAGTTCAAGGAGCTGCTGACCAAAAAGGGCAGCT
ACAGATACTACTTCAAGAAAGTGAGCGACGAGTTTGACTGTGGGGTGGTG
TTTGAGGAGGTTCGAGAGGACGAGGCCGTCCTGCCCGTCTTTGAGGAGAA
GATCATCGGCAAAGTGGAGAAGGTGGACCATCATCACCATCACCATTAAG
AATTC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSPGHRSPDSGHVAKMPVALG
GAASGHGKHVPKSGAKLDAAGLHHHRHVHHHVHHSTARPKEQVEAEATRR
AQSSFAWGLEPHSHGARSRGYSESVGAAPNASDGLAHSGKVGVACKRNAK
KAESGKSASTEVPGASEDAEKNQKIMQWIIEGEKEISRHRRTGHGSSGTR
KPQPHENSRPLSLEHPWAGPQLRTSVQPSHLFIQDPTMPPHPAPNPLTQL
EEARRRLEEEEKRASRAPSKQRYVQEVMRRGRACVRPACAPVLHVVPAVS
DMELSETETRSQRKVGGGSAQPCDSIVVAYYFCGEPIPYRTLVRGRAVTL
GQFKELLTKKGSYRYYFKKVSDEFDCGVVFEEVREDEAVLPVFEEKIIGK
VEKVDHHHHHH*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU1721
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N terminal GST C terminal 6His for bacterial expression.; Estimated MW of expressed protein : 68691 Daltons