cDNA Clone ERK8

• Synonyms: MAPK15
• Plasmid: pGEX6P-1-ERK8 6His D154A
• Parent Plasmid: pGEX6P1
• Expressed: GST-ERK8 6His D154A
• Genbank: AY065978
• Tag: GST
• Species: Human

Sequence of Insert

GGATCCTGCACCGTAGTGGACCCTCGCATTGTCCGGAGATACCTACTCAG
GCGGCAGCTCGGGCAGGGGGCCTATGGCATTGTGTGGAAGGCAGTGGACC
GGAGGACTGGTGAGGTCGTGGCCATCAAGAAAATCTTTGATGCTTTTAGG
GATAAGACAGATGCCCAGAGAACATTCCGGGAAATCACGCTCCTCCAGGA
GTTTGGGGACCATCCCAACATCATCAGCCTCCTTGACGTGATCCGGGCAG
AGAACGACAGGGACATTTACCTGGTGTTTGAGTTTATGGACACTGACCTG
AACGCAGTCATCCGGAAGGGCGGCCTGCTGCAGGACGTCCACGTGCGCTC
CATCTTCTACCAGCTCCTGCGGGCCACCCGGTTCCTCCACTCGGGGCACG
TTGTGCACCGGGACCAGAAGCCGTCCAATGTGCTCCTGGATGCCAACTGC
ACAGTGAAGCTGTGTGCCTTTGGCCTGGCCCGCTCCCTGGGCGACCTCCC
TGAGGGGCCTGAGGACCAGGCCGTGACAGAGTACGTGGCCACACGCTGGT
ACCGAGCACCGGAGGTGCTGCTCTCTTCGCACCGATACACCCTTGGGGTG
GACATGTGGAGTCTGGGCTGTATCCTGGGGGAGATGCTGCGGGGGAGACC
CCTGTTCCCCGGCACGTCCACCCTCCACCAGCTGGAGCTGATCCTGGAGA
CCATCCCACCGCCATCTGAGGAGGACCTCCTGGCTCTCGGCTCAGGCTGC
CGTGCCTCTGTGCTGCACCAGCTGGGGTCCCGGCCACGACAGACGCTGGA
TGCCCTCCTACCGCCAGACACCTCCCCAGAGGCCTTGGACCTCCTTAGGC
GACTCCTGGTGTTCGCCCCGGACAAGCGGTTAAGCGCGACCCAGGCACTG
CAGCACCCCTACGTGCAGAGGTTCCACTGCCCCAGCGACGAGTGGGCACG
AGAGGCAGATGTGCGGCCCCGGGCACACGAAGGGGTCCAGCTCTCTGTGC
CTGAGTACCGCAGCCGCGTCTATCAGATGATCCTGGAGTGTGGAGGCAGC
AGCGGCACCTCGAGAGAGAAGGGCCCGGAGGGTGTCTCCCCAAGCCAGGC
ACACCTGCACAAACCCAGAGCCGACCCTCAGCTGCCTTCTAGGACACCTG
TGCAGGGTCCCAGACCCAGGCCCCAGAGCAGCCCAGGCCATGACCCTGCC
GAGCACGAGTCCCCCCGTGCAGCCAAGAACGTTCCCAGGCAGAACTCCGC
TCCCCTGCTCCAAACTGCTCTCCTAGGGAATGGGGAAAGGCCCCCTGGGG
CGAAGGAAGCGCCCCCCTTGACACTCTCGCTGGTGAAGCCAAGCGGGAGG
GGAGCTGCGCCCTCCCTGACCTCCCAGGCTGCGGCTCAGGTGGCCAACCA
GGCCCTGATCCGGGGTGACTGGAACCGGGGCGGTGGGGTGAGGGTGGCCA
GCGTACAACAGGTCCCTCCCCGGCTTCCTCCGGAGGCCCGGCCCGGCCGG
AGGATGTTCAGCACCTCTGCCTTGCAGGGTGCCCAGGGGGGTGCCAGGGC
TTTGCTTGGAGGCTACTCCCAAGCCTACGGGACTGTCTGCCACTCGGCAC
TGGGCCACCTGCCCCTGCTGGAGGGGCACCATGTGCACCATCACCATCAC
CATTGAGAATTCCG

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSCTVVDPRIVRRYLLRRQLG
QGAYGIVWKAVDRRTGEVVAIKKIFDAFRDKTDAQRTFREITLLQEFGDH
PNIISLLDVIRAENDRDIYLVFEFMDTDLNAVIRKGGLLQDVHVRSIFYQ
LLRATRFLHSGHVVHRDQKPSNVLLDANCTVKLCAFGLARSLGDLPEGPE
DQAVTEYVATRWYRAPEVLLSSHRYTLGVDMWSLGCILGEMLRGRPLFPG
TSTLHQLELILETIPPPSEEDLLALGSGCRASVLHQLGSRPRQTLDALLP
PDTSPEALDLLRRLLVFAPDKRLSATQALQHPYVQRFHCPSDEWAREADV
RPRAHEGVQLSVPEYRSRVYQMILECGGSSGTSREKGPEGVSPSQAHLHK
PRADPQLPSRTPVQGPRPRPQSSPGHDPAEHESPRAAKNVPRQNSAPLLQ
TALLGNGERPPGAKEAPPLTLSLVKPSGRGAAPSLTSQAAAQVANQALIR
GDWNRGGGVRVASVQQVPPRLPPEARPGRRMFSTSALQGAQGGARALLGG
YSQAYGTVCHSALGHLPLLEGHHVHHHHHH*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU1720
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial expression construct to express N terminal GST / C terminal 6His fusion