cDNA Clone AMPK (rat)

• Plasmid: pGEX-AMPK 3-307 T172D
• Parent Plasmid: pGEX4T1
• Expressed: GST-AMPK 3-307
• Genbank: U40819
• Tag: GST
• Species: Rat

Sequence of Insert

GGATCCGAATTCGCCATGGAGCAGAAGCTTATCTCCGAGGAGGACCTCGG
TGGCGGCGAGAAGCAGAAGCACGACGGGCGGGTGAAGATCGGCCACTACA
TCCTGGGGGACACGCTGGGCGTCGGCACCTTCGGGAAAGTGAAGGTGGGC
AAGCACGAGTTGACTGGACATAAAGTTGCTGTGAAGATACTCAACCGGCA
GAAGATTCGAAGCCTGGACGTGGTCGGGAAAATCCGCAGAGAGATCCAGA
ACCTGAAGCTTTTCAGGCACCCTCATATAATCAAACTGTACCAGGTCATC
AGTACACCGTCTGATATTTTCATGGTCATGGAATATGTCTCAGGAGGAGA
GCTATTTGATTATATCTGTAAAAATGGAAGGTTGGACGAAAAGGAGAGTC
GACGTCTGTTCCAGCAGATCCTTTCTGGTGTGGACTATTGTCACAGGCAT
ATGGTGGTCCACAGAGATTTGAAACCTGAAAACGTCCTGCTTGATGCACA
CATGAATGCAAAGATAGCCGACTTCGGTCTTTCAAACATGATGTCAGATG
GTGAATTTTTAAGAGATAGCTGTGGCTCGCCCAATTATGCTGCACCAGAA
GTAATTTCAGGAAGATTGTACGCAGGCCCTGAAGTAGACATCTGGAGCAG
CGGGGTCATTCTCTATGCTTTGCTGTGTGGAACTCTCCCTTTTGATGATG
ACCACGTGCCAACTCTTTTTAAGAAGATATGTGACGGGATATTTTATACC
CCTCAGTATTTGAATCCCTCTGTAATAAGCCTTTTGAAGCATATGCTGCA
GGTAGATCCTATGAAGAGGGCCACAATAAAAGATATCAGGGAACATGAAT
GGTTTAAGCAGGACCTTCCAAAATATCTCTTTCCTGAAGACCCGTCTTAT
AGTTCAACCATGATTGATGATGAAGCCTTAAAAGAAGTGTGTGAGAAGTT
CGAGTGCTCAGAGGAGGAGGTCCTCAGATCCATCACACTGGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLVPRGSEFAMEQKLISEEDLGGGEKQKHDG
RVKIGHYILGDTLGVGTFGKVKVGKHELTGHKVAVKILNRQKIRSLDVVG
KIRREIQNLKLFRHPHIIKLYQVISTPSDIFMVMEYVSGGELFDYICKNG
RLDEKESRRLFQQILSGVDYCHRHMVVHRDLKPENVLLDAHMNAKIADFG
LSNMMSDGEFLRDSCGSPNYAAPEVISGRLYAGPEVDIWSSGVILYALLC
GTLPFDDDHVPTLFKKICDGIFYTPQYLNPSVISLLKHMLQVDPMKRATI
KDIREHEWFKQDLPKYLFPEDPSYSSTMIDDEALKEVCEKFECSEEEVLR
SITLAAARDRLD*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU1713
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee