cDNA Clone NUCB1

• Plasmid: pGEX6P-3-NUCB1
• Parent Plasmid: pGEX6P3
• Expressed: GST-NUCB1
• Genbank: NM_006184
• Tag: GST
• Species: Human

Sequence of Insert

GTCGACCCTCCCTCTGGGCCCCGAGGAACCCTCCTTCTGTTGCCGCTGCT
GCTGCTGCTCCTGCTTCGCGCCGTGCTGGCTGTCCCCCTGGAGCGAGGGG
CGCCCAACAAGGAGGAGACCCCTGCGACTGAGAGTCCCGACACAGGCCTG
TACTACCACCGGTACCTCCAGGAGGTCATCGATGTACTGGAGACGGATGG
GCATTTCCGAGAGAAGCTGCAGGCTGCCAATGCGGAGGACATCAAGAGCG
GGAAGCTGAGCCGAGAGCTGGACTTTGTCAGCCACCACGTCCGCACCAAG
CTGGATGAGCTCAAGCGACAGGAGGTGTCACGGCTGCGGATGCTGCTCAA
GGCCAAGATGGACGCCGAGCAGGATCCCAATGTACAGGTGGATCATCTGA
ATCTCCTGAAACAGTTTGAACACCTGGACCCTCAGAACCAGCATACATTC
GAGGCCCGCGACCTGGAGCTGCTGATCCAGACGGCCACCCGGGACCTTGC
CCAGTACGACGCAGCCCATCATGAAGAGTTCAAGCGCTACGAGATGCTTA
AGGAACACGAGAGACGGCGTTATCTGGAGTCACTGGGAGAGGAGCAGAGA
AAGGAGGCGGAGAGGAAGCTGGAAGAGCAACAGCGCCGGCACCGCGAGCA
CCCTAAAGTCAACGTGCCTGGCAGCCAAGCCCAGTTGAAGGAGGTGTGGG
AGGAGCTGGATGGACTGGACCCCAACAGGTTTAACCCCAAGACCTTCTTC
ATACTGCATGATATCAACAGTGATGGTGTCCTGGATGAGCAGGAGCTGGA
GGCACTCTTCACCAAGGAGCTGGAGAAAGTGTACGACCCAAAGAATGAGG
AGGACGACATGCGGGAGATGGAGGAGGAGCGACTGCGCATGCGGGAGCAT
GTGATGAAGAATGTGGACACCAACCAGGACCGCCTCGTGACCCTGGAGGA
GTTCCTCGCATCCACTCAGAGGAAGGAGTTTGGGGACACCGGGGAGGGCT
GGGAGACAGTGGAGATGCACCCTGCCTACACCGAGGAAGAGCTGAGGCGC
TTTGAAGAGGAGCTGGCTGCCCGGGAGGCAGAGCTGAATGCCAAGGCCCA
GCGCCTCAGCCAGGAGACAGAGGCTCTAGGGCGGTCCCAGGGCCGCCTGG
AGGCCCAGAAGAGAGAGCTGCAGCAGGCTGTGCTGCACATGGAGCAGCGG
AAGCAGCAGCAGCAGCAGCAGCAAGGCCACAAGGCCCCGGCTGCCCACCC
TGAGGGGCAGCTCAAGTTCCACCCAGACACAGACGATGTACCTGTCCCAG
CTCCAGCCGGTGACCAGAAGGAGGTGGACACTTCAGAAAAGAAACTTCTC
GAGCGGCTCCCTGAGGTTGAGGTGCCCCAGCATCTGTGAGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSPNSRVDPPSGPRGTLLLLP
LLLLLLLRAVLAVPLERGAPNKEETPATESPDTGLYYHRYLQEVIDVLET
DGHFREKLQAANAEDIKSGKLSRELDFVSHHVRTKLDELKRQEVSRLRML
LKAKMDAEQDPNVQVDHLNLLKQFEHLDPQNQHTFEARDLELLIQTATRD
LAQYDAAHHEEFKRYEMLKEHERRRYLESLGEEQRKEAERKLEEQQRRHR
EHPKVNVPGSQAQLKEVWEELDGLDPNRFNPKTFFILHDINSDGVLDEQE
LEALFTKELEKVYDPKNEEDDMREMEEERLRMREHVMKNVDTNQDRLVTL
EEFLASTQRKEFGDTGEGWETVEMHPAYTEEELRRFEEELAAREAELNAK
AQRLSQETEALGRSQGRLEAQKRELQQAVLHMEQRKQQQQQQQGHKAPAA
HPEGQLKFHPDTDDVPVPAPAGDQKEVDTSEKKLLERLPEVEVPQHL*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU1692
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial expression construct with N terminal GST tag.