cDNA Clone DBF4

• Plasmid: pGEX6P-1-DBF4
• Parent Plasmid: pGEX6P1
• Expressed: GST-DBF4
• Genbank: AB028069
• Tag: GST
• Species: Human

Sequence of Insert

GAATTCAACTCCGGAGCCATGAGGATCCACAGTAAAGGACATTTCCAGGG
TGGAATCCAAGTCAAAAATGAAAAAAACAGACCATCTCTGAAATCTCTGA
AAACTGATAACAGGCCAGAAAAATCCAAATGTAAGCCACTTTGGGGAAAA
GTATTTTACCTTGACTTACCTTCTGTCACCATATCTGAAAAACTTCAAAA
GGACATTAAGGATCTGGGAGGGCGAGTTGAAGAATTTCTCAGCAAAGATA
TCAGTTATCTTATTTCAAATAAGAAGGAAGCTAAATTTGCACAAACCTTG
GGTCGAATTTCTCCTGTACCAAGTCCAGAATCTGCATATACTGCAGAAAC
CACTTCACCTCATCCCAGCCATGATGGAAGTTCATTTAAGTCACCAGACA
CAGTGTGTTTAAGCAGAGGAAAATTATTAGTTGAAAAAGCTATCAAGGAC
CATGATTTTATTCCTTCAAATAGTATATTATCAAATGCCTTGTCATGGGG
AGTAAAAATTCTTCATATTGATGACATTAGATACTACATTGAACAAAAGA
AAAAAGAGTTGTATTTACTCAAGAAATCAAGTACTTCAGTAAGAGATGGG
GGCAAAAGAGTTGGTAGTGGTGCACAAAAAACAAGAACAGGAAGACTCAA
AAAGCCTTTTGTAAAGGTGGAAGATATGAGCCAACTTTATAGGCCATTTT
ATCTTCAGCTGACCAATATGCCTTTTATAAATTATTCTATTCAGAAGCCC
TGCAGTCCATTTGATGTAGACAAGCCATCTAGTATGCAAAAGCAAACTCA
GGTTAAACTAAGAATCCAAACAGATGGCGATAAGTATGGTGGAACCTCAA
TTCAACTCCAGTTGAAAGAGAAGAAGAAAAAAGGATATTGTGAATGTTGC
TTGCAGAAATATGAAGATCTAGAAACTCACCTTCTAAGTGAGCAACACAG
AAACTTTGCACAGAGTAACCAGTATCAAGTTGTTGATGATATTGTATCTA
AGTTAGTTTTTGACTTTGTGGAATATGAAAAGGACACACCTAAAAAGAAA
AGAATAAAATACAGTGTTGGATCCCTTTCTCCTGTTTCTGCAAGTGTCCT
GAAAAAGACTGAACAAAAGGAAAAAGTGGAATTGCAACATATTTCTCAGA
AAGATTGCCAGGAAGATGATACAACAGTGAAGGAGCAGAATTTCCTGTAT
AAAGAGACCCAGGAAACTGAAAAAAAGCTCCTGTTTATTTCAGAGCCCAT
CCCCCACCCTTCAAATGAATTGAGAGGGCTTAATGAGAAAATGAGTAATA
AATGTTCCATGTTAAGTACAGCTGAAGATGACATAAGACAGAATTTTACA
CAGCTACCTCTACATAAAAACAAACAGGAATGCATTCTTGACATTTCCGA
ACACACATTAAGTGAAAATGACTTAGAAGAACTAAGGGTAGATCACTATA
AATGTAACATACAGGCATCTGTACATGTTTCTGATTTCAGTACAGATAAT
AGTGGATCTCAACCAAAACAGAAGTCAGATACTGTGCTTTTTCCAGCAAA
GGATCTCAAGGAAAAGGACCTTCATTCAATATTTACTCATGATTCTGGTC
TGATAACAATAAACAGTTCACAAGAGCACCTAACTGTTCAGGCAAAGGCT
CCATTCCATACTCCTCCTGAGGAACCCAATGAATGTGACTTCAAGAATAT
GGATAGTTTACCTTCTGGTAAAATACATCGAAAAGTGAAAATAATATTAG
GACGAAATAGAAAAGAAAATCTGGAACCAAATGCTGAATTTGATAAAAGA
ACTGAATTTATTACACAAGAAGAAAACAGAATTTGTAGTTCACCGGTACA
GTCTTTACTAGACTTGTTTCAGACTAGTGAAGAGAAATCAGAATTTTTGG
GTTTCACAAGCTACACAGAAAAGAGTGGTATATGCAATGTTTTAGATATT
TGGGAAGAGGAAAATTCAGATAATCTGTTAACAGCGTTTTTCTCGTCCCC
TTCAACTTCTACATTTACTGGCTTTTAGCTCGAG

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSPEFNSGAMRIHSKGHFQGG
IQVKNEKNRPSLKSLKTDNRPEKSKCKPLWGKVFYLDLPSVTISEKLQKD
IKDLGGRVEEFLSKDISYLISNKKEAKFAQTLGRISPVPSPESAYTAETT
SPHPSHDGSSFKSPDTVCLSRGKLLVEKAIKDHDFIPSNSILSNALSWGV
KILHIDDIRYYIEQKKKELYLLKKSSTSVRDGGKRVGSGAQKTRTGRLKK
PFVKVEDMSQLYRPFYLQLTNMPFINYSIQKPCSPFDVDKPSSMQKQTQV
KLRIQTDGDKYGGTSIQLQLKEKKKKGYCECCLQKYEDLETHLLSEQHRN
FAQSNQYQVVDDIVSKLVFDFVEYEKDTPKKKRIKYSVGSLSPVSASVLK
KTEQKEKVELQHISQKDCQEDDTTVKEQNFLYKETQETEKKLLFISEPIP
HPSNELRGLNEKMSNKCSMLSTAEDDIRQNFTQLPLHKNKQECILDISEH
TLSENDLEELRVDHYKCNIQASVHVSDFSTDNSGSQPKQKSDTVLFPAKD
LKEKDLHSIFTHDSGLITINSSQEHLTVQAKAPFHTPPEEPNECDFKNMD
SLPSGKIHRKVKIILGRNRKENLEPNAEFDKRTEFITQEENRICSSPVQS
LLDLFQTSEEKSEFLGFTSYTEKSGICNVLDIWEEENSDNLLTAFFSSPS
TSTFTGF*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU1669
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial construct to express GST N terminal fusion