cDNA Clone PRK2

• Synonyms: PKN2
• Plasmid: pFBHTb PRK2 501-985
• Parent Plasmid: pFastBac HTb
• Expressed: 6His-PRK2 501-985
• Genbank: S75548
• Tag: 6His
• Species: Human

Sequence of Insert

GGATCCGCCACCATGGACTACAAGGACGACGATGACAAGGGCAAAACATT
TCTCAGAGCTCCTCAAATGAATATTAATATTGCCACTTGGGGAAGGCTAG
TAAGAAGAGCTATTCCTACAGTAAATCATTCTGGCACCTTCAGCCCTCAA
GCTCCTGTGCCTACTACAGTGCCAGTGGTTGATGTACGCATCCCTCAACT
AGCACCTCCAGCTAGTGATTCTACAGTAACCAAATTGGACTTTGATCTTG
AGCCTGAACCTCCTCCAGCCCCACCACGAGCTTCTTCTCTTGGAGAAATA
GATGAATCTTCTGAATTAAGAGTTTTGGATATACCAGGACAGGATTCAGA
GACTGTTTTTGATATTCAGAATGACAGAAATAGTATACTTCCAAAATCTC
AATCTGAATACAAGCCTGATACTCCTCAGTCAGGCCTAGAATATAGTGGT
ATTCAAGAACTTGAGGACAGAAGATCTCAGCAAAGGTTTCAGTTTAATCT
ACAAGATTTCAGGTGTTGTGCTGTCTTGGGAAGAGGACATTTTGGAAAGG
TGCTTTTAGCTGAATATAAAAACACAAATGAGATGTTTGCTATAAAAGCC
TTAAAGAAAGGAGATATTGTGGCTCGAGATGAAGTAGACAGCCTGATGTG
TGAAAAAAGAATTTTTGAAACTGTGAATAGTGTAAGGCATCCCTTTTTGG
TGAACCTTTTTGCATGTTTCCAAACCAAAGAGCATGTTTGCTTTGTAATG
GAATATGCTGCCGGTGGGGACCTAATGATGCACATTCATACTGATGTCTT
TTCTGAACCAAGAGCTGTATTTTATGCTGCTTGTGTAGTTCTTGGGTTGC
AGTATTTACATGAACACAAAATTGTTTATAGAGATTTGAAATTGGATAAC
TTATTGCTAGATACAGAGGGCTTTGTGAAAATTGCTGATTTTGGTCTTTG
CAAAGAAGGAATGGGATATGGAGATAGAACAAGCACATTTTGTGGCACTC
CTGAATTTCTTGCCCCAGAAGTATTAACAGAAACTTCTTATACAAGGGCT
GTAGATTGGTGGGGCCTTGGCGTGCTTATATATGAAATGCTTGTTGGTGA
GTCTCCCTTTCCTGGTGATGATGAAGAGGAAGTTTTTGACAGTATTGTAA
ATGATGAAGTAAGGTATCCAAGGTTCTTATCTACAGAAGCCATTTCTATA
ATGAGAAGGCTGTTAAGAAGAAATCCTGAACGGCGCCTTGGGGCTAGCGA
GAAAGATGCAGAGGATGTAAAAAAGCACCCATTTTTCCGGCTAATTGATT
GGAGCGCTCTGATGGACAAAAAAGTAAAGCCACCATTTATACCTACCATA
AGAGGACGAGAAGATGTTAGTAATTTTGATGATGAATTTACCTCAGAAGC
ACCTATTCTGACTCCACCTCGAGAACCAAGGATACTTTCGGAAGAGGAGC
AGGAAATGTTCAGAGATTTTGACTACATTGCTGATTGGTGTTAAGAATTC

Amino Acid Sequence

MSYYHHHHHHDYDIPTTENLYFQGAMGSATMDYKDDDDKGKTFLRAPQMN
INIATWGRLVRRAIPTVNHSGTFSPQAPVPTTVPVVDVRIPQLAPPASDS
TVTKLDFDLEPEPPPAPPRASSLGEIDESSELRVLDIPGQDSETVFDIQN
DRNSILPKSQSEYKPDTPQSGLEYSGIQELEDRRSQQRFQFNLQDFRCCA
VLGRGHFGKVLLAEYKNTNEMFAIKALKKGDIVARDEVDSLMCEKRIFET
VNSVRHPFLVNLFACFQTKEHVCFVMEYAAGGDLMMHIHTDVFSEPRAVF
YAACVVLGLQYLHEHKIVYRDLKLDNLLLDTEGFVKIADFGLCKEGMGYG
DRTSTFCGTPEFLAPEVLTETSYTRAVDWWGLGVLIYEMLVGESPFPGDD
EEEVFDSIVNDEVRYPRFLSTEAISIMRRLLRRNPERRLGASEKDAEDVK
KHPFFRLIDWSALMDKKVKPPFIPTIRGREDVSNFDDEFTSEAPILTPPR
EPRILSEEEQEMFRDFDYIADWC*

• Vector Type: Baculovirus
• Antibiotic: Amp
• DU Number: DU1658
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N terminal 6His/FLAG tag for baculovirus expression