cDNA Clone F3K

• Plasmid: pCMV5-HA F3K
• Parent Plasmid: pCMV5
• Expressed: HA-F3K
• Genbank: AK022233
• Tag: HA
• Species: Human

Sequence of Insert

GTCGACGCCACCATGTACCCATACGATGTGCCAGATTACGCCGAGGAGCT
GCTGAGGCGCGAGCTGGGCTGCAGCTCTGTCAGGGCCACGGGCCACTCGG
GGGGCGGGTGCATCAGCCAGGGCCGGAGCTACGACACGGATCAAGGACGA
GTGTTCGTGAAAGTGAACCCCAAGGCGGAGGCCAGAAGAATGTTTGAAGG
TGAGATGGCAAGTTTAACTGCCATCCTGAAAACAAACACGGTGAAAGTGC
CCAAGCCCATCAAGGTTCTGGATGCCCCAGGCGGCGGGAGCGTGCTGGTG
ATGGAGCACATGGACATGAGGCATCTGAGCAGTCATGCTGCAAAGCTTGG
AGCCCAGCTGGCCGATTTACACCTTGATAACAAGAAGCTTGGAGAGATGC
GCCTGAAGGAGGCGGGCACAGTGGGGAGAGGAGGTGGGCAGGAGGAACGG
CCCTTTGTGGCCCGGTTTGGATTTGACGTGGTGACGTGCTGTGGATACCT
CCCCCAGGTGAATGACTGGCAGGAGGACTGGGTCGTGTTCTATGCCCGGC
AGCGCATTCAGCCCCAGATGGACATGGTGGAGAAGGAGTCTGGGGACAGG
GAGGCCCTCCAGCTTTGGTCTGCTCTGCAGTTAAAGATCCCTGACCTGTT
CCGTGACCTGGAGATCATCCCAGCCTTACTCCACGGGGACCTCTGGGGTG
GAAACGTAGCAGAGGATTCCTCTGGGCCGGTGATTTTTGACCCAGCTTCT
TTCTACGGCCACTCGGAATATGAGCTGGCAATAGCTGGCATGTTTGGGGG
CTTTAGCAGCTCCTTTTACTCCGCCTACCACGGCAAAATCCCCAAGGCCC
CAGGATTCGAGAAGCGCCTTCGGTTGTATCAGCTCTTTCACTACTTGAAC
CACTGGAATCATTTTGGATCGGGGTACAGAGGATCCTCCCTGAACATCAT
GAGGAATCTGGTCAAGTGAGTCGAC

Amino Acid Sequence

MYPYDVPDYAEELLRRELGCSSVRATGHSGGGCISQGRSYDTDQGRVFVK
VNPKAEARRMFEGEMASLTAILKTNTVKVPKPIKVLDAPGGGSVLVMEHM
DMRHLSSHAAKLGAQLADLHLDNKKLGEMRLKEAGTVGRGGGQEERPFVA
RFGFDVVTCCGYLPQVNDWQEDWVVFYARQRIQPQMDMVEKESGDREALQ
LWSALQLKIPDLFRDLEIIPALLHGDLWGGNVAEDSSGPVIFDPASFYGH
SEYELAIAGMFGGFSSSFYSAYHGKIPKAPGFEKRLRLYQLFHYLNHWNH
FGSGYRGSSLNIMRNLVK*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU1654
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N Terminal HA fusion for mammalian expression