cDNA Clone MTHFR

• Plasmid: pGEX6P-1-HA MTHFR Corrected
• Parent Plasmid: pGEX6P1
• Expressed: GST-HA MTHFR Corrected
• Genbank: U09806
• Tag: GST
• Tag: HA
• Species: Human

Sequence of Insert

AGATCTGCCACCATGTACCCATACGATGTGCCAGATTACGCCGTGAACGA
AGCCAGAGGAAACAGCAGCCTCAACCCCTGCTTGGAGGGCAGTGCCAGCA
GTGGCAGTGAGAGCTCCAAAGATAGTTCGAGATGTTCCACCCCGGGCCTG
GACCCCGAGCGGCATGAGAGACTCCGGGAGAAGATGAGGCGGCGATTGGA
ATCTGGTGACAAGTGGTTCTCCCTGGAATTCTTCCCTCCTCGAACTGCTG
AGGGAGCTGTCAATCTCATCTCAAGGTTTGACCGGATGGCAGCAGGTGGC
CCCCTCTACATAGACGTGACCTGGCACCCAGCAGGTGACCCTGGCTCAGA
CAAGGAGACCTCCTCCATGATGATCGCCAGCACCGCCGTGAACTACTGTG
GCCTGGAGACCATCCTGCACATGACCTGCTGCCGTCAGCGCCTGGAGGAG
ATCACGGGCCATCTGCACAAAGCTAAGCAGCTGGGCCTGAAGAACATCAT
GGCGCTGCGGGGAGACCCAATAGGTGACCAGTGGGAAGAGGAGGAGGGAG
GCTTCAACTACGCAGTGGACCTGGTGAAGCACATCCGAAGTGAGTTTGGT
GACTACTTTGACATCTGTGTGGCAGGTTACCCCAAAGGCCACCCCGAAGC
AGGGAGCTTTGAGGCTGACCTGAAGCACTTGAAGGAGAAGGTGTCTGCGG
GAGCCGATTTCATCATCACGCAGCTTTTCTTTGAGGCTGACACATTCTTC
CGCTTTGTGAAGGCATGCACCGACATGGGCATCACTTGCCCCATCGTCCC
CGGGATCTTTCCCATCCAGGGCTACCACTCCCTTCGGCAGCTTGTGAAGC
TGTCCAAGCTGGAGGTGCCACAGGAGATCAAGGACGTGATTGAGCCAATC
AAAGACAACGATGCTGCCATCCGCAACTATGGCATCGAGCTGGCCGTGAG
CCTGTGCCAGGAGCTTCTGGCCAGTGGCTTGGTGCCAGGCCTCCACTTCT
ACACCCTCAACCGCGAGATGGCTACCACAGAGGTGCTGAAGCGCCTGGGG
ATGTGGACTGAGGACCCCAGGCGTCCCCTACCCTGGGCTCTCAGCGCCCA
CCCCAAGCGCCGAGAGGAAGATGTACGTCCCATCTTCTGGGCCTCCAGAC
CAAAGAGTTACATCTACCGTACCCAGGAGTGGGACGAGTTCCCTAACGGC
CGCTGGGGCAATTCCTCTTCCCCTGCCTTTGGGGAGCTGAAGGACTACTA
CCTCTTCTACCTGAAGAGCAAGTCCCCCAAGGAGGAGCTGCTGAAGATGT
GGGGGGAGGAGCTGACCAGTGAAGCAAGTGTCTTTGAAGTCTTTGTTCTT
TACCTCTCGGGAGAACCAAACCGGAATGGTCACAAAGTGACTTGCCTGCC
CTGGAACGATGAGCCCCTGGCGGCTGAGACCAGCCTGCTGAAGGAGGAGC
TGCTGCGGGTGAACCGCCAGGGCATCCTCACCATCAACTCACAGCCCAAC
ATCAACGGGAAGCCGTCCTCCGACCCCATCGTGGGCTGGGGCCCCAGCGG
GGGCTATGTCTTCCAGAAGGCCTACTTAGAGTTTTTCACTTCCCGCGAGA
CAGCGGAAGCACTTCTGCAAGTGCTGAAGAAGTACGAGCTCCGGGTTAAT
TACCACCTTGTCAATGTGAAGGGTGAAAACATCACCAATGCCCCTGAACT
GCAGCCGAATGCTGTCACTTGGGGCATCTTCCCTGGGCGAGAGATCATCC
AGCCCACCGTAGTGGATCCCGTCAGCTTCATGTTCTGGAAGGACGAGGCC
TTTGCCCTGTGGATTGAGCGGTGGGGAAAGCTGTATGAGGAGGAGTCCCC
GTCCCGCACCATCATCCAGTACATCCACGACAACTACTTCCTGGTCAACC
TGGTGGACAATGACTTCCCACTGGACAACTGCCTCTGGCAGGTGGTGGAA
GACACATTGGAGCTTCTCAACAGGCCCACCCAGAATGCGAGAGAAACGGA
GGCTCCATGACCCTGCGTCCTGACGCCCTGCGTTGGAGCCACTCCTGTCC
CGCCTTCCTCCTCCACAGTGCTGCTTCTCTTGGGAACTCCACTCTCCTTC
GTGTCTCTCCCACCCCGGCCTCCACTCCCCCACCTGACAATGGCAGCTAG
ACTGGAGTGAGGCTTCCAGGCTCTTCCTGGACCTGAGTCGGCCCCACATG
GGAACCTAGTACTCTCTGCTCTAAGATCT

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMYPYDVPDYAVNEARGNSS
LNPCLEGSASSGSESSKDSSRCSTPGLDPERHERLREKMRRRLESGDKWF
SLEFFPPRTAEGAVNLISRFDRMAAGGPLYIDVTWHPAGDPGSDKETSSM
MIASTAVNYCGLETILHMTCCRQRLEEITGHLHKAKQLGLKNIMALRGDP
IGDQWEEEEGGFNYAVDLVKHIRSEFGDYFDICVAGYPKGHPEAGSFEAD
LKHLKEKVSAGADFIITQLFFEADTFFRFVKACTDMGITCPIVPGIFPIQ
GYHSLRQLVKLSKLEVPQEIKDVIEPIKDNDAAIRNYGIELAVSLCQELL
ASGLVPGLHFYTLNREMATTEVLKRLGMWTEDPRRPLPWALSAHPKRREE
DVRPIFWASRPKSYIYRTQEWDEFPNGRWGNSSSPAFGELKDYYLFYLKS
KSPKEELLKMWGEELTSEASVFEVFVLYLSGEPNRNGHKVTCLPWNDEPL
AAETSLLKEELLRVNRQGILTINSQPNINGKPSSDPIVGWGPSGGYVFQK
AYLEFFTSRETAEALLQVLKKYELRVNYHLVNVKGENITNAPELQPNAVT
WGIFPGREIIQPTVVDPVSFMFWKDEAFALWIERWGKLYEEESPSRTIIQ
YIHDNYFLVNLVDNDFPLDNCLWQVVEDTLELLNRPTQNARETEAP*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU1650
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial construct to express GST/HA N terminal fusion.