cDNA Clone CHK2

• Synonyms: CHEK2
• Plasmid: pGEX-CHK2
• Parent Plasmid: pGEX6P1
• Expressed: GST-CHK2
• Genbank: Q13164
• Tag: GST
• Species: Human

Sequence of Insert

GGATCCGCCGAGCCTCTGAAGGAGGAAGACGGCGAGGACGGCTCTGCGGA
GCCCCCGGCCCGTGAAGGTCGAACCCGCCCACACCGCTGCCTCTGTAGCG
CCAAGAACCTGGCCCTGCTTAAAGCCCGCTCCTTCGATGTGACCTTTGAC
GTGGGCGACGAGTACGAGATCATCGAGACCATAGGCAACGGGGCCTATGG
AGTGGTGTCCTCCGCCCGCCGCCGCCTCACCGGCCAGCAGGTGGCCATCA
AGAAGATCCCTAATGCTTTCGATGTGGTGACCAATGCCAAGCGGACCCTC
AGGGAGCTGAAGATCCTCAAGCACTTTAAACACGACAACATCATCGCCAT
CAAGGACATCCTGAGGCCCACCGTGCCCTATGGCGAATTCAAATCTGTCT
ACGTGGTCCTGGACCTGATGGAAAGCGACCTGCACCAGATCATCCACTCC
TCACAGCCCCTCACACTGGAACACGTGCGCTACTTCCTGTACCAACTGCT
GCGGGGCCTGAAGTACATGCACTCGGCTCAGGTCATCCACCGTGACCTGA
AGCCCTCCAACCTATTGGTGAATGAGAACTGTGAGCTCAAGATTGGTGAC
TTTGGTATGGCTCGTGGCCTGTGCACCTCGCCCGCTGAACATCAGTACTT
CATGACTGAGTATGTGGCCACGCGCTGGTACCGTGCGCCCGAGCTCATGC
TCTCTTTGCATGAGTATACACAGGCTATTGACCTCTGGTCTGTGGGCTGC
ATCTTTGGTGAGATGCTGGCCCGGCGCCAGCTCTTCCCAGGCAAAAACTA
TGTACACCAGCTACAGCTCATCATGATGGTGCTGGGTACCCCATCACCAG
CCGTGATTCAGGCTGTGGGGGCTGAGAGGGTGCGGGCCTATATCCAGAGC
TTGCCACCACGCCAGCCTGTGCCCTGGGAGACAGTGTACCCAGGTGCCGA
CCGCCAGGCCCTATCACTGCTGGGTCGCATGCTGCGTTTTGAGCCCAGCG
CTCGCATCTCAGCAGCTGCTGCCCTTCGCCACCCTTTCCTGGCCAAGTAC
CATGATCCTGATGATGAGCCTGACTGTGCCCCGCCCTTTGACTTTGCCTT
TGACCGCGAAGCCCTCACTCGGGAGCGCATTAAGGAGGCCATTGTGGCTG
AAATTGAGGACTTCCATGCAAGGCGTGAGGGCATCCGCCAACAGATCCGC
TTCCAGCCTTCTCTACAGCCTGTGGCTAGTGAGCCTGGCTGTCCAGATGT
TGAAATGCCCAGTCCCTGGGCTCCCAGTGGGGACTGTGCCATGGAGTCTC
CACCACCAGCCCCGCCACCATGCCCCGGCCCTGCACCTGACACCATTGAT
CTGACCCTGCAGCCACCTCCACCAGTCAGTGAGCCTGCCCCACCAAAGAA
AGATGGTGCCATCTCAGACAATACTAAGGCTGCCCTTAAAGCTGCCCTGC
TCAAGTCTTTGAGGAGCCGGCTCAGAGATGGCCCCAGCGCACCCCTGGAG
GCTCCTGAGCCTCGGAAGCCGGTGACAGCCCAGGAGCGCCAGCGGGAGCG
GGAGGAGAAGCGGCGGAGGCGGCAAGAACGAGCCAAGGAGCGGGAGAAAC
GGCGGCAGGAGCGGGAGCGAAAGGAACGGGGGGCTGGGGCCTCTGGGGGC
CCCTCCACTGACCCCTTGGCTGGACTAGTGCTCAGTGACAATGACAGAAG
CCTGTTGGAACGCTGGACTCGAATGGCCCGGCCCGCAGCCCCAGCCCTCA
CCTCTGTGCCGGCCCCTGCCCCAGCGCCAACGCCAACCCCAACCCCAGTC
CAACCTACCAGTCCTCCTCCTGGCCCTCTAGCCCAGCCCACTGGCCCGCA
ACCACAATCTGCGGGCTCTACCTCTGGCCCTGTACCCCAGCCTGCCTGCC
CACCCCCTGGCCCTGCACCCCACCCCACTGGCCCTCCTGGGCCCATCCCT
GTCCCCGCGCCACCCCAGATTGCCACCTCCACCAGCCTCCTGGCTGCCCA
GTCACTTGTGCCACCCCCTGGGCTGCCTGGCTCCAGCACCCCAGGAGTTT
TGCCTTACTTCCCACCTGGCCTGCCGCCCCCAGACGCCGGGGGAGCCCCT
CAGTCTTCCATGTCAGAGTCACCTGATGTCAACCTTGTGACCCAGCAGCT
ATCTAAGTCACAGGTGGAGGACCCCCTGCCCCCTGTGTTCTCAGGCACAC
CAAAGGGCAGTGGGGCTGGCTACGGTGTTGGCTTTGACCTGGAGGAATTC
TTAAACCAGTCTTTCGACATGGGCGTGGCTGATGGGCCACAGGATGGCCA
GGCAGATTCAGCCTCTCTCTCAGCCTCCCTGCTTGCTGACTGGCTCGAAG
GCCATGGCATGAACCCTGCCGATATTGAGTCCCTGCAGCGTGAGATCCAG
ATGGACTCCCCAATGCTGCTGGCTGACCTGCCTGACCTCCAGGACCCCCA
CCATCACCATCACCATGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSAEPLKEEDGEDGSAEPPAR
EGRTRPHRCLCSAKNLALLKARSFDVTFDVGDEYEIIETIGNGAYGVVSS
ARRRLTGQQVAIKKIPNAFDVVTNAKRTLRELKILKHFKHDNIIAIKDIL
RPTVPYGEFKSVYVVLDLMESDLHQIIHSSQPLTLEHVRYFLYQLLRGLK
YMHSAQVIHRDLKPSNLLVNENCELKIGDFGMARGLCTSPAEHQYFMTEY
VATRWYRAPELMLSLHEYTQAIDLWSVGCIFGEMLARRQLFPGKNYVHQL
QLIMMVLGTPSPAVIQAVGAERVRAYIQSLPPRQPVPWETVYPGADRQAL
SLLGRMLRFEPSARISAAAALRHPFLAKYHDPDDEPDCAPPFDFAFDREA
LTRERIKEAIVAEIEDFHARREGIRQQIRFQPSLQPVASEPGCPDVEMPS
PWAPSGDCAMESPPPAPPPCPGPAPDTIDLTLQPPPPVSEPAPPKKDGAI
SDNTKAALKAALLKSLRSRLRDGPSAPLEAPEPRKPVTAQERQREREEKR
RRRQERAKEREKRRQERERKERGAGASGGPSTDPLAGLVLSDNDRSLLER
WTRMARPAAPALTSVPAPAPAPTPTPTPVQPTSPPPGPLAQPTGPQPQSA
GSTSGPVPQPACPPPGPAPHPTGPPGPIPVPAPPQIATSTSLLAAQSLVP
PPGLPGSSTPGVLPYFPPGLPPPDAGGAPQSSMSESPDVNLVTQQLSKSQ
VEDPLPPVFSGTPKGSGAGYGVGFDLEEFLNQSFDMGVADGPQDGQADSA
SLSASLLADWLEGHGMNPADIESLQREIQMDSPMLLADLPDLQDPHHHHH
H*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU1663
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial construct to express GST N terminal and 6His C terminal fusion