cDNA Clone CK1 delta (rat)

• Synonyms: CSNK1D
• Plasmid: pGEX-CK1delta
• Parent Plasmid: pGEX4T1
• Expressed: GST-CK1delta
• Genbank: AB063114
• Tag: GST
• Species: Rat

Sequence of Insert

GAATTCGGCTTATGGAGCTGAGGGTCGGGAATAGGTACCGACTGGGCCGC
AAGATCGGCAGCGGCTCCTTCGGAGACATCTATCTCGGTACGGACATTGC
TGCAGGAGAAGAAGTTGCCATCAAGCTTGAATGTGTCAAAACCAAACATC
CTCAGCTCCACATTGAGAGCAAGATCTACAAAATGATGCAGGGAGGAGTG
GGCATCCCTACCATCAGATGGTGTGGGGCTGAGGGGGACTACAATGTCAT
GGTGATGGAGCTACTGGGACCCAGCCTGGAAGACCTATTCAACTTCTGTT
CAAGGAAGTTTAGTCTCAAAACTGTTCTGTTGCTTGCTGACCAAATGATA
AGTCGTATTGAGTACATTCATTCGAAGAATTTTATCCACCGAGACGTGAA
ACCAGATAACTTCCTCATGGGGCTGGGAAAGAAAGGCAACCTGGTCTACA
TCATTGACTTTGGGCTGGCCAAGAAGTATCGGGATGCCCGCACCCACCAG
CATATCCCCTATCGAGAGAACAAGAACCTCACAGGGACAGCACGCTATGC
CTCCATCAACACACACCTTGGCATTGAACAATCTCGAAGGGATGACTTGG
AGTCTCTGGGGTACGTGCTGATGTACTTCAACCTGGGCTCTCTCCCCTGG
CAGGGGCTGAAGGCCGCCACCAAAAGGCAGAGGTATGAGAGGATCAGTGA
GAAGAAGATGTCCACTCCAATTGAAGTGCTGTGCAAGGGCTATCCTTCTG
AATTTGCCACATACCTGAATTTCTGCCGTTCCTTACGTTTTGATGACAAA
CCTGACTACTCCTACCTGAGACAGCTCTTCAGAAATCTGTTCCATCGCCA
GGGCTTCTCCTACGACTATGTGTTCGACTGGAACATGCTCAAATGAGGTG
CCACCCGGGCCCGAGCCCTCAGCTCCATAAGCCGAATTC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLVPRGSPGIRLMELRVGNRYRLGRKIGSGS
FGDIYLGTDIAAGEEVAIKLECVKTKHPQLHIESKIYKMMQGGVGIPTIR
WCGAEGDYNVMVMELLGPSLEDLFNFCSRKFSLKTVLLLADQMISRIEYI
HSKNFIHRDVKPDNFLMGLGKKGNLVYIIDFGLAKKYRDARTHQHIPYRE
NKNLTGTARYASINTHLGIEQSRRDDLESLGYVLMYFNLGSLPWQGLKAA
TKRQRYERISEKKMSTPIEVLCKGYPSEFATYLNFCRSLRFDDKPDYSYL
RQLFRNLFHRQGFSYDYVFDWNMLK*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU1632
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N terminal GST fusion for bacterial expression. Encodes aa1-294 of genbank reference.