cDNA Clone NIK

• Plasmid: pEBG-2T-NIK CAT
• Parent Plasmid: pEBG2T
• Expressed: GST-NIK CAT
• Genbank: Q99558
• Tag: GST
• Species: Human

Sequence of Insert

GGATCCTGGGCCACGCACCAGCTCCGCCTGGGCAGAGGCTCCTTCGGAGA
GGTGCACAGGATGGAGGACAAGCAGACTGGCTTCCAGTGCGCTGTCAAAA
AGGTGCGGCTGGAAGTATTTCGGGCAGAGGAGCTGATGGCATGTGCAGGA
TTGACCTCACCCAGAATTGTCCCTTTGTATGGAGCTGTGAGAGAAGGGCC
TTGGGTCAACATCTTCATGGAGCTGCTGGAAGGTGGCTCCCTGGGCCAGC
TGGTCAAGGAGCAGGGCTGTCTCCCAGAGGACCGGGCCCTGTACTACCTG
GGCCAGGCCCTGGAGGGTCTGGAATACCTCCACTCACGAAGGATTCTGCA
TGGGGACGTCAAAGCTGACAACGTGCTCCTGTCCAGCGATGGGAGCCACG
CAGCCCTCTGTGACTTTGGCCATGCTGTGTGTCTTCAACCTGATGGCCTG
GGAAAGTCCTTGCTCACAGGGGACTACATCCCTGGCACAGAGACCCACAT
GGCTCCGGAGGTGGTGCTGGGCAGGAGCTGCGACGCCAAGGTGGATGTCT
GGAGCAGCTGCTGTATGATGCTGCACATGCTCAACGGCTGCCACCCCTGG
ACTCAGTTCTTCCGAGGGCCGCTCTGCCTCAAGATTGCCAGCGAGCCTCC
GCCTGTGAGGGAGATCCCACCCTCCTGCGCCCCTCTCACAGCCCAGGCCA
TCCAAGAGGGGCTGAGGAAAGAGCCCATCCACCGCGTGTCTGCAGCGGAG
CTGGGAGGGAAGGTGAACCGGGCACTACAGTAAGAATTC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLVPRGSWATHQLRLGRGSFGEVHRMEDKQT
GFQCAVKKVRLEVFRAEELMACAGLTSPRIVPLYGAVREGPWVNIFMELL
EGGSLGQLVKEQGCLPEDRALYYLGQALEGLEYLHSRRILHGDVKADNVL
LSSDGSHAALCDFGHAVCLQPDGLGKSLLTGDYIPGTETHMAPEVVLGRS
CDAKVDVWSSCCMMLHMLNGCHPWTQFFRGPLCLKIASEPPPVREIPPSC
APLTAQAIQEGLRKEPIHRVSAAELGGKVNRALQ*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU1627
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Mammalian expression plasmid with N terminal GST tag