cDNA Clone Laforin

• Synonyms: EPM2; MELF; EPM2A
• Plasmid: pGEX6P1 Laforin
• Parent Plasmid: pGEX6P1
• Expressed: GST 3C Laforin
• Genbank: NP_005661
• Tag: GST
• Species: Human

Sequence of Insert

GGATCCCGCTTCCGCTTTGGGGTGGTGGTGCCACCCGCCGTGGCCGGCGC
CCGGCCGGAGCTGCTGGTGGTGGGGTCGCGGCCCGAGCTGGGGCGTTGGG
AGCCGCGCGGTGCCGTCCGCCTGAGGCCGGCCGGCACCGCGGCGGGCGAC
GGGGCCCTGGCGCTGCAGGAGCCGGGCCTGTGGCTCGGGGAGGTGGAGCT
GGCGGCCGAGGAGGCGGCGCAGGACGGGGCGGAGCCGGGCCGCGTGGACA
CGTTCTGGTACAAGTTCCTGAAGCGGGAGCCGGGAGGAGAGCTCTCCTGG
GAAGGCAATGGACCTCATCATGACCGTTGCTGTACTTACAATGAAAACAA
CTTGGTGGATGGTGTGTATTGTCTCCCAATAGGACACTGGATTGAGGCCA
CTGGACACACCAATGAAATGAAGCACACAACAGACTTCTATTTTAATATT
GCAGGCCACCAAGCCATGCATTATTCAAGAATTCTACCAAATATCTGGCT
GGGTAGCTGCCCTCGTCAGGTGGAACATGTAACCATCAAACTGAAGCATG
AATTGGGGATTACAGCTGTAATGAATTTCCAGACTGAATGGGATATTGTA
CAGAATTCCTCAGGCTGTAACCGCTACCCAGAGCCCATGACTCCAGACAC
TATGATTAAACTATATAGGGAAGAAGGCTTGGCCTACATCTGGATGCCAA
CACCAGATATGAGCACCGAAGGCCGAGTACAGATGCTGCCCCAGGCGGTG
TGCCTGCTGCATGCGCTGCTGGAGAAGGGACACATCGTGTACGTGCACTG
CAACGCTGGGGTGGGCCGCTCCACCGCGGCTGTCTGCGGCTGGCTCCAGT
ATGTGATGGGCTGGAATCTGAGGAAGGTGCAGTATTTCCTCATGGCCAAG
AGGCCGGCTGTCTACATTGACGAAGAGGCCTTGGCCCGGGCACAAGAAGA
TTTTTTCCAGAAATTTGGGAAGGTTCGTTCTTCTGTGTGTAGCCTGTAGG
GATCC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSRFRFGVVVPPAVAGARPEL
LVVGSRPELGRWEPRGAVRLRPAGTAAGDGALALQEPGLWLGEVELAAEE
AAQDGAEPGRVDTFWYKFLKREPGGELSWEGNGPHHDRCCTYNENNLVDG
VYCLPIGHWIEATGHTNEMKHTTDFYFNIAGHQAMHYSRILPNIWLGSCP
RQVEHVTIKLKHELGITAVMNFQTEWDIVQNSSGCNRYPEPMTPDTMIKL
YREEGLAYIWMPTPDMSTEGRVQMLPQAVCLLHALLEKGHIVYVHCNAGV
GRSTAAVCGWLQYVMGWNLRKVQYFLMAKRPAVYIDEEALARAQEDFFQK
FGKVRSSVCSL*

• Vector Type: Bacterial
• Antibiotic: Amp
• Full Snapgene Map: pGEX6P1 Laforin56.02 KB Snapgene files can be opened using software available here: www.snapgene.com
• DU Number: DU1620
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N terminal GST tag for bacterial expression.
• Low Temp: No