cDNA Clone METTL1

• Plasmid: pFBHTb METTL1
• Parent Plasmid: pFastBac HTb
• Expressed: 6His-METTL1
• Genbank: Y18643
• Tag: 6His

Sequence of Insert

GGATCCGCAGCCGAGACTCGGAACGTGGCCGGAGCAGAGGCCCCACCGCC
CCAGAAGCGCTACTACCGGCAACGTGCTCACTCCAACCCCATGGCGGACC
ACACGCTGCGCTACCCTGTGAAGCCAGAGGAGATGGACTGGTCTGAGCTA
TACCCAGAGTTCTTCGCTCCACTCACTCAAAATCAGAGCCACGATGACCC
AAAGGATAAGAAAGAAAAGAGAGCTCAGGCCCAAGTGGAGTTTGCAGACA
TAGGCTGTGGCTATGGTGGCCTGTTAGTGGAACTGTCACCGCTGTTCCCA
GACACACTTATTCTGGGTCTGGAGATCCGGGTGAAGGTCTCAGACTATGT
ACAAGACCGGATTCGGGCCCTACGCGCAGCTCCTGCAGGTGGCTTCCAGA
ACATCGCCTGTCTCCGTAGCAATGCCATGAAGCACCTTCCTAACTTCTTC
TACAAGGGCCAGCTGACAAAGATGTTCTTCCTCTTCCCCGACCCACATTT
CAAGCGGACAAAGCACAAGTGGCGAATCATCAGTCCCACCCTGCTAGCAG
AATATGCCTACGTGCTAAGAGTTGGGGGGCTGGTGTATACCATAACCGAT
GTGCTGGAGCTACACGACTGGATGTGCACTCATTTCGAAGAGCACCCACT
GTTTGAGCGTGTGCCTCTGGAGGACCTGAGTGAAGACCCCGTTGTGGGAC
ATCTAGGCACCTCAACTGAGGAGGGGAAGAAAGTTCTACGTAATGGAGGG
AAGAATTTCCCAGCCATCTTCCGAAGAATACAAGATCCCGTCCTCCAGGC
AGTGACCTCCCAAACCAGCCTGCCTGGTCACTGAGGATCC

Amino Acid Sequence

MSYYHHHHHHDYDIPTTENLYFQGAMGSAAETRNVAGAEAPPPQKRYYRQ
RAHSNPMADHTLRYPVKPEEMDWSELYPEFFAPLTQNQSHDDPKDKKEKR
AQAQVEFADIGCGYGGLLVELSPLFPDTLILGLEIRVKVSDYVQDRIRAL
RAAPAGGFQNIACLRSNAMKHLPNFFYKGQLTKMFFLFPDPHFKRTKHKW
RIISPTLLAEYAYVLRVGGLVYTITDVLELHDWMCTHFEEHPLFERVPLE
DLSEDPVVGHLGTSTEEGKKVLRNGGKNFPAIFRRIQDPVLQAVTSQTSL
PGH*

• Vector Type: Baculovirus
• DU Number: DU1618
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Baculovirus construct to express 6His N terminal fusion