cDNA Clone WDR4

• Plasmid: pMal-p2X-6His WDR4
• Parent Plasmid: pMALP2X
• Expressed: MBP-6His-WDR4
• Genbank: BC001074
• Tag: MBP
• Species: Human

Sequence of Insert

GGATCCCACCATCACCATCACCATCTGTTAGATGTGGCTGTGAGTCCTGA
TGACCGCTTCATCCTCACTGCCGACCGGGACGAGAAGATCCGAGTCAGCT
GGGCCGCGGCGCCCCATAGCATCGAGTCCTTCTGCTTGGGGCACACAGAG
TTTGTGAGCCGTATCTCCGTGGTGCCAACTCAGCCCGGGCTGCTTCTGTC
CTCCTCTGGGGACGGCACCCTGAGGCTCTGGGAGTACAGGAGCGGCCGCC
AGCTGCACTGCTGTCACCTGGCCAGTCTGCAGGAGCTGGTGGACCCCCAG
GCCCCCCAGAAGTTTGCCGCGTCCAGGATTGCATTCTGGTGCCAGGAGAA
CTGCGTGGCGCTCCTGTGCGACGGCACTTCTGTGGTCTACATCTTCCAGC
TGGACGCCCGCAGACAGCAGTTGGTGTACAGGCAGCAGCTGGCGTTCCAG
CACCAAGTGTGGGACGTGGCTTTCGAGGAGACCCAGGGGCTGTGGGTGCT
CCAGGACTGCCAGGAAGCCCCCCTGGTGCTCTACAGGCCTGTGGGCGACC
AGTGGCAGTCTGTTCCTGAGAGCACCGTGTTAAAGAAAGTCTCTGGTGTT
CTTCGTGGGAACTGGGCCATGCTGGAAGGCTCTGCCGGCGCAGACGCCAG
CTTCAGCAGTCTCTACAAGGCCACGTTCGACAACGTGACCTCCTACCTGA
AGAAGAAAGAGGAGAGACTGCAGCAGCAGCTAGAGAAGAAGCAGCGGCGC
CGGAGTCCCCCGCCTGGGCCCGACGGGCATGCCAAGAAGATGAGACCGGG
GGAGGCGACGCTAAGTTGCTGAGGATCC

Amino Acid Sequence

MKIKTGARILALSALTTMMFSASALAKIEEGKLVIWINGDKGYNGLAEVG
KKFEKDTGIKVTVEHPDKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSG
LLAEITPDKAFQDKLYPFTWDAVRYNGKLIAYPIAVEALSLIYNKDLLPN
PPKTWEEIPALDKELKAKGKSALMFNLQEPYFTWPLIAADGGYAFKYENG
KYDIKDVGVDNAGAKAGLTFLVDLIKNKHMNADTDYSIAEAAFNKGETAM
TINGPWAWSNIDTSKVNYGVTVLPTFKGQPSKPFVGVLSAGINAASPNKE
LAKEFLENYLLTDEGLEAVNKDKPLGAVALKSYEEELAKDPRIAATMENA
QKGEIMPNIPQMSAFWYAVRTAVINAASGRQTVDEALKDAQTNSSSNNNN
NNNNNNLGIEGRISEFGSHHHHHHLLDVAVSPDDRFILTADRDEKIRVSW
AAAPHSIESFCLGHTEFVSRISVVPTQPGLLLSSSGDGTLRLWEYRSGRQ
LHCCHLASLQELVDPQAPQKFAASRIAFWCQENCVALLCDGTSVVYIFQL
DARRQQLVYRQQLAFQHQVWDVAFEETQGLWVLQDCQEAPLVLYRPVGDQ
WQSVPESTVLKKVSGVLRGNWAMLEGSAGADASFSSLYKATFDNVTSYLK
KKEERLQQQLEKKQRRRSPPPGPDGHAKKMRPGEATLSC*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU1617
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial construct to express N terminal MBP/6His fusion