cDNA Clone PDK1

• Plasmid: pFBHTb PDK1 FL
• Parent Plasmid: pFastBac HTb
• Expressed: 6His-PDK1 FL
• Genbank: AF017995
• Tag: 6His
• Species: Human

Sequence of Insert

GGATCTGCCACCATGGAGCAGAAGCTGATCTCTGAAGAGGACTTGGCCAG
GACCACCAGCCAGCTGTATGACGCCGTGCCCATCCAGTCCAGCGTGGTGT
TATGTTCCTGCCCATCCCCATCAATGGTGAGGACCCAGACTGAGTCCAGC
ACGCCCCCTGGCATTCCTGGTGGCAGCAGGCAGGGCCCCGCCATGGACGG
CACTGCAGCCGAGCCTCGGCCCGGCGCCGGCTCCCTGCAGCATGCCCAGC
CTCCGCCGCAGCCTCGGAAGAAGCGGCCTGAGGACTTCAAGTTTGGGAAA
ATCCTTGGGGAAGGCTCTTTTTCCACGGTTGTCCTGGCTCGAGAACTGGC
AACCTCCAGAGAATATGCGATTAAAATTCTGGAGAAGCGACATATCATAA
AAGAGAACAAGGTCCCCTATGTAACCAGAGAGCGGGATGTCATGTCGCGC
CTGGATCACCCCTTCTTTGTTAAGCTTTACTTCACATTTCAGGACGACGA
GAAGCTGTATTTCGGCCTTAGTTATGCCAAAAATGGAGAACTACTTAAAT
ATATTCGCAAAATCGGTTCATTCGATGAGACCTGTACCCGATTTTACACG
GCTGAGATCGTGTCTGCTTTAGAGTACTTGCACGGCAAGGGCATCATTCA
CAGGGACCTTAAACCGGAAAACATTTTGTTAAATGAAGATATGCACATCC
AGATCACAGATTTTGGAACAGCAAAAGTCTTATCCCCAGAGAGCAAACAA
GCCAGGGCCAACTCATTCGTGGGAACAGCGCAGTACGTTTCTCCAGAGCT
GCTCACGGAGAAGTCCGCCTGTAAGAGTTCAGACCTTTGGGCTCTTGGAT
GCATAATATACCAGCTTGTGGCAGGACTCCCACCATTCCGAGCTGGAAAC
GAGTATCTTATATTTCAGAAGATCATTAAGTTGGAATATGACTTTCCAGA
AAAATTCTTCCCTAAGGCAAGAGACCTCGTGGAGAAACTTTTGGTTTTAG
ATGCCACAAAGCGGTTAGGCTGTGAGGAAATGGAAGGATACGGACCTCTT
AAAGCACACCCGTTCTTCGAGTCCGTCACGTGGGAGAACCTGCACCAGCA
GACGCCTCCGAAGCTCACCGCTTACCTGCCGGCTATGTCGGAAGACGACG
AGGACTGCTATGGCAATTATGACAATCTCCTGAGCCAGTTTGGCTGCATG
CAGGTGTCTTCGTCCTCCTCCTCACACTCCCTGTCAGCCTCCGACACGGG
CCTGCCCCAGAGGTCAGGCAGCAACATAGAGCAGTACATTCACGATCTGG
ACTCGAACTCCTTTGAACTGGACTTACAGTTTTCCGAAGATGAGAAGAGG
TTGTTGTTGGAGAAGCAGGCTGGCGGAAACCCTTGGCACCAGTTTGTAGA
AAATAATTTAATACTAAAGATGGGCCCAGTGGATAAGCGGAAGGGTTTAT
TTGCAAGACGACGACAGCTGTTGCTCACAGAAGGACCACATTTATATTAT
GTGGATCCTGTCAACAAAGTTCTGAAAGGTGAAATTCCTTGGTCACAAGA
ACTTCGACCAGAGGCCAAGAATTTTAAAACTTTCTTTGTCCACACGCCTA
ACAGGACGTATTATCTGATGGACCCCAGCGGGAACGCACACAAGTGGTGC
AGGAAGATCCAGGAGGTTTGGAGGCAGCGATACCAGAGCCACCCGGACGC
CGCTGTGCAGTGA

Amino Acid Sequence

MSYYHHHHHHDYDIPTTENLYFQGAMGSATMEQKLISEEDLARTTSQLYD
AVPIQSSVVLCSCPSPSMVRTQTESSTPPGIPGGSRQGPAMDGTAAEPRP
GAGSLQHAQPPPQPRKKRPEDFKFGKILGEGSFSTVVLARELATSREYAI
KILEKRHIIKENKVPYVTRERDVMSRLDHPFFVKLYFTFQDDEKLYFGLS
YAKNGELLKYIRKIGSFDETCTRFYTAEIVSALEYLHGKGIIHRDLKPEN
ILLNEDMHIQITDFGTAKVLSPESKQARANSFVGTAQYVSPELLTEKSAC
KSSDLWALGCIIYQLVAGLPPFRAGNEYLIFQKIIKLEYDFPEKFFPKAR
DLVEKLLVLDATKRLGCEEMEGYGPLKAHPFFESVTWENLHQQTPPKLTA
YLPAMSEDDEDCYGNYDNLLSQFGCMQVSSSSSSHSLSASDTGLPQRSGS
NIEQYIHDLDSNSFELDLQFSEDEKRLLLEKQAGGNPWHQFVENNLILKM
GPVDKRKGLFARRRQLLLTEGPHLYYVDPVNKVLKGEIPWSQELRPEAKN
FKTFFVHTPNRTYYLMDPSGNAHKWCRKIQEVWRQRYQSHPDAAVQ

• Vector Type: Baculovirus
• Antibiotic: Amp
• DU Number: DU1600
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N terminal 6His/myc tag for baculovirus expression.