cDNA Clone RhoGEF16

• Plasmid: pEGFP-C1-HA-RhoGef16
• Parent Plasmid: pEGFPC1
• Expressed: GFP-HA RhoGef16
• Genbank: NM_014448
• Tag: GFP
• Tag: HA
• Species: Human

Sequence of Insert

GGATCCGCCACCATGTACCCATACGATGTGCCAGATTACGCCTTCGAGAT
CCTCACGTCGGAGTTCTCCTACCAGCACAGCCTGAGCATCCTGGTGGAGG
AGTTCCTGCAGTCCAAGGAGCTGCGGGCGACCGTGACCCAGATGGAGCAC
CACCACCTCTTCTCCAACATCCTGGATGTCCTGGGTGCCAGTCAGAGGTT
CTTCGAGGACCTGGAGCAGCGGCACAAGGCCCAGGTGCTGGTCGAGGACA
TCAGTGACATCCTGGAGGAGCACGCTGAGAAGTACTTCCACCCCTACATC
GCCTACTGCTCCAACGAGGTCTACCAACAGCGCACGCTGCAGAAGCTGAT
AAGCAGCAACGCCGCCTTCCGAGAGGCCCTGAGAGAGATTGAGAGGCGGC
CGGCGTGCGGGGGCCTGCCCATGCTCTCCTTCCTGATCCTCCCCATGCAG
CGGGTGACCCGGCTGCCCCTCCTGATGGATACGCTCTGCCTCAAGACCCA
GGGCCACTCCGAAAGGTACAAGGCTGCCAGCCGTGCACTGAAGGCCATCA
GCAAGCTGGTGAGGCAGTGCAACGAGGGGGCCCACAGGATGGAGCGCATG
GAGCAGATGTACACGCTGCACACACAGCTGGACTTCAGCAAGGTCAAGTC
CCTCCCACTGATCTCCGCCTCCCGGTGGCTGCTGAAGCGCGGAGAGCTGT
TCTTAGTGGAAGAAACCGGACTTTTTCGAAAAATTGCCAGCCGGCCAACG
TGCTACCTTTTCCTGTTCAACGATGTCCTGGTTGTGACCAAGAAGAAGAG
CGAGGAGAGCTACATGGTCCAGGACTACGCCCAGATGAACCACATCCAGG
TAGAGAAGATAGAGCCGTCTGAGCTCCCTCTGCCCGGGGGCGGCAACCGT
AGCTCCTCCGTGCCCCACCCCTTCCAGGTGACCCTGCTTCGCAACAGCGA
GGGCCGCCAGGAGCAGCTCCTGCTCTCCTCGGACTCCGCGAGTGACCGGG
CACGGTGGATCGTGGCGCTCACACACAGTGAGAGACAGTGGCAGGGCCTC
TCCAGCAAAGGAGACCTGCCCCAGGTGGAGATCACCAAGGCCTTCTTCGC
GAAGCAAGCAGACGAGGTCACACTGCAGCAGGCGGACGTGGTCCTGGTTC
TGCAGCAGGAGGATGGGTGGCTCTATGGCGAGAGGCTCCGGGACGGAGAG
ACGGGATGGTTCCCCGAGGACTTTGCCCGCTTCATCACCAGCCGTGTGGC
CGTGGAGGGCAATGTCCGCAGGATGGAGCGTCTGCGGGTGGAGACGGACG
TGTAGGGATCC

Amino Acid Sequence

MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICT
TGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIF
FKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHN
VYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
YLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKSGLRSRAQASN
SAVDGTAGPGSMYPYDVPDYAFEILTSEFSYQHSLSILVEEFLQSKELRA
TVTQMEHHHLFSNILDVLGASQRFFEDLEQRHKAQVLVEDISDILEEHAE
KYFHPYIAYCSNEVYQQRTLQKLISSNAAFREALREIERRPACGGLPMLS
FLILPMQRVTRLPLLMDTLCLKTQGHSERYKAASRALKAISKLVRQCNEG
AHRMERMEQMYTLHTQLDFSKVKSLPLISASRWLLKRGELFLVEETGLFR
KIASRPTCYLFLFNDVLVVTKKKSEESYMVQDYAQMNHIQVEKIEPSELP
LPGGGNRSSSVPHPFQVTLLRNSEGRQEQLLLSSDSASDRARWIVALTHS
ERQWQGLSSKGDLPQVEITKAFFAKQADEVTLQQADVVLVLQQEDGWLYG
ERLRDGETGWFPEDFARFITSRVAVEGNVRRMERLRVETDV*

• Vector Type: Mammalian
• Antibiotic: Kan
• DU Number: DU1595
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N terminal GFP/HA tag for mammalian expression