cDNA Clone PA28a

• Plasmid: pEGFP-C1-PA28a
• Parent Plasmid: pEGFPC1
• Expressed: GFP-PA28a
• Genbank: NM_006263
• Tag: GFP
• Species: Human

Sequence of Insert

GGATCCGCCATGCTCAGGGTCCAGCCCGAGGCCCAAGCCAAGGTGGATGT
GTTTCGTGAAGACCTCTGTACCAAGACAGAGAACCTGCTCGGGAGCTATT
TCCCCAAGAAGATTTCTGAGCTGGATGCATTTTTAAAGGAGCCAGCTCTC
AATGAAGCCAACTTGAGCAATCTGAAGGCCCCATTGGACATCCCAGTGCC
TGATCCAGTCAAGGAGAAAGAGAAAGAGGAGCGGAAGAAACAGCAGGAGA
AGGAAGACAAGGATGAAAAGAAGAAGGGGGAGGATGAAGACAAAGGTCCT
CCCTGTGGCCCAGTGAACTGCAATGAAAAGATCGTGGTCCTTCTGCAGCG
CTTGAAGCCTGAGATCAAGGATGTCATTGAGCAGCTCAACCTGGTCACCA
CCTGGTTGCAGCTGCAGATACCTCGGATTGAGGATGGTAACAATTTTGGA
GTGGCTGTCCAGGAGAAGGTGTTTGAGCTGATGACCAGCCTCCACACCAA
GCTAGAAGGCTTCCACACTCAAATCTCTAAGTATTTCTCTGAGCGTGGTG
ATGCAGTGACTAAAGCAGCCAAGCAGCCCCATGTGGGTGATTATCGGCAG
CTGGTGCACGAGCTGGATGAGGCAGAGTACCGGGACATCCGGCTGATGGT
CATGGAGATCCGCAATGCTTATGCTGTGTTATATGACATCATCCTGAAGA
ACTTCGAGAAGCTCAAGAAGCCCAGGGGAGAAACAAAGGGAATGATCTAT
TGAGGATCC

Amino Acid Sequence

MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICT
TGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIF
FKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHN
VYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
YLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKSGLRSRAQASN
SAVDGTAGPGSTGSAMLRVQPEAQAKVDVFREDLCTKTENLLGSYFPKKI
SELDAFLKEPALNEANLSNLKAPLDIPVPDPVKEKEKEERKKQQEKEDKD
EKKKGEDEDKGPPCGPVNCNEKIVVLLQRLKPEIKDVIEQLNLVTTWLQL
QIPRIEDGNNFGVAVQEKVFELMTSLHTKLEGFHTQISKYFSERGDAVTK
AAKQPHVGDYRQLVHELDEAEYRDIRLMVMEIRNAYAVLYDIILKNFEKL
KKPRGETKGMIY*

• Vector Type: Mammalian
• Antibiotic: Kan
• DU Number: DU1567
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N Terminal GFP fusion for mammalian expression