cDNA Clone PA28a

• Plasmid: pGEX6P-1-PA28a
• Parent Plasmid: pGEX6P1
• Expressed: GST-PA28a
• Genbank: NM_006263
• Tag: GST

Sequence of Insert

GGATCCGCCATGCTCAGGGTCCAGCCCGAGGCCCAAGCCAAGGTGGATGT
GTTTCGTGAAGACCTCTGTACCAAGACAGAGAACCTGCTCGGGAGCTATT
TCCCCAAGAAGATTTCTGAGCTGGATGCATTTTTAAAGGAGCCAGCTCTC
AATGAAGCCAACTTGAGCAATCTGAAGGCCCCATTGGACATCCCAGTGCC
TGATCCAGTCAAGGAGAAAGAGAAAGAGGAGCGGAAGAAACAGCAGGAGA
AGGAAGACAAGGATGAAAAGAAGAAGGGGGAGGATGAAGACAAAGGTCCT
CCCTGTGGCCCAGTGAACTGCAATGAAAAGATCGTGGTCCTTCTGCAGCG
CTTGAAGCCTGAGATCAAGGATGTCATTGAGCAGCTCAACCTGGTCACCA
CCTGGTTGCAGCTGCAGATACCTCGGATTGAGGATGGTAACAATTTTGGA
GTGGCTGTCCAGGAGAAGGTGTTTGAGCTGATGACCAGCCTCCACACCAA
GCTAGAAGGCTTCCACACTCAAATCTCTAAGTATTTCTCTGAGCGTGGTG
ATGCAGTGACTAAAGCAGCCAAGCAGCCCCATGTGGGTGATTATCGGCAG
CTGGTGCACGAGCTGGATGAGGCAGAGTACCGGGACATCCGGCTGATGGT
CATGGAGATCCGCAATGCTTATGCTGTGTTATATGACATCATCCTGAAGA
ACTTCGAGAAGCTCAAGAAGCCCAGGGGAGAAACAAAGGGAATGATCTAT
TGAGGATCC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSAMLRVQPEAQAKVDVFRED
LCTKTENLLGSYFPKKISELDAFLKEPALNEANLSNLKAPLDIPVPDPVK
EKEKEERKKQQEKEDKDEKKKGEDEDKGPPCGPVNCNEKIVVLLQRLKPE
IKDVIEQLNLVTTWLQLQIPRIEDGNNFGVAVQEKVFELMTSLHTKLEGF
HTQISKYFSERGDAVTKAAKQPHVGDYRQLVHELDEAEYRDIRLMVMEIR
NAYAVLYDIILKNFEKLKKPRGETKGMIY*

• Vector Type: Bacterial
• DU Number: DU1565
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N Terminal GST fusion for bacterial expression