cDNA Clone NDRG1

• Plasmid: pGEX6P-1-NDRG1
• Parent Plasmid: pGEX6P1
• Expressed: GST-NDRG1
• Genbank: XP_005243
• Tag: GST
• Species: Human

Sequence of Insert

GGATCCGCCACCATGGACTACAAGGACGACGATGACAAGTCTCGGGAGAT
GCAGGATGTAGACCTCGCTGAGGTGAAGCCTTTGGTGGAGAAAGGGGAGA
CCATCACCGGCCTCCTGCAAGAGTTTGATGTCCAGGAGCAGGACATCGAG
ACTTTACATGGCTCTGTTCACGTCACGCTGTGTGGGACTCCCAAGGGAAA
CCGGCCTGTCATCCTCACCTACCATGACATCGGCATGAACCACAAAACCT
GCTACAACCCCCTCTTCAACTACGAGGACATGCAGGAGATCACCCAGCAC
TTTGCCGTCTGCCACGTGGACGCCCCTGGCCAGCAGGACGGCGCAGCCTC
CTTCCCCGCAGGGTACATGTACCCCTCCATGGATCAGCTGGCTGAAATGC
TTCCTGGAGTCCTTCAACAGTTTGGGCTGAAAAGCATTATTGGCATGGGA
ACAGGAGCAGGCGCCTACATCCTAACTCGATTTGCTCTAAACAACCCTGA
GATGGTGGAGGGCCTTGTCCTTATCAACGTGAACCCTTGTGCGGAAGGCT
GGATGGACTGGGCCGCCTCCAAGATCTCAGGATGGACCCAAGCTCTGCCG
GACATGGTGGTGTCCCACCTTTTTGGGAAGGAAGAAATGCAGAGTAACGT
GGAAGTGGTCCACACCTACCGCCAGCACATTGTGAATGACATGAACCCCG
GCAACCTGCACCTGTTCATCAATGCCTACAACAGCCGGCGCGACCTGGAG
ATTGAGCGACCAATGCCGGGAACCCACACAGTCACCCTGCAGTGCCCTGC
TCTGTTGGTGGTTGGGGACAGCTCGCCTGCAGTGGATGCCGTGGTGGAGT
GCAACTCAAAATTGGACCCAACAAAGACCACTCTCCTCAAGATGGCGGAC
TGTGGCGGCCTCCCGCAGATCTCCCAGCCGGCCAAGCTCGCTGAGGCCTT
CAAGTACTTCGTGCAGGGCATGGGATACATGCCCTCGGCTAGCATGACCC
GCCTGATGCGGTCCCGCACAGCCTCTGGTTCCAGCGTCACTTCTCTGGAT
GGCACCCGCAGCCGCTCCCACACCAGCGAGGGCACCCGAAGCCGCTCCCA
CACCAGCGAGGGCACCCGCAGCCGCTCGCACACCAGCGAGGGGGCCCACC
TGGACATCACCCCCAACTCGGGTGCTGCTGGGAACAGCGCCGGGCCCAAG
TCCATGGAGGTCTCCTGCTAGGGATCC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSATMDYKDDDDKSREMQDVD
LAEVKPLVEKGETITGLLQEFDVQEQDIETLHGSVHVTLCGTPKGNRPVI
LTYHDIGMNHKTCYNPLFNYEDMQEITQHFAVCHVDAPGQQDGAASFPAG
YMYPSMDQLAEMLPGVLQQFGLKSIIGMGTGAGAYILTRFALNNPEMVEG
LVLINVNPCAEGWMDWAASKISGWTQALPDMVVSHLFGKEEMQSNVEVVH
TYRQHIVNDMNPGNLHLFINAYNSRRDLEIERPMPGTHTVTLQCPALLVV
GDSSPAVDAVVECNSKLDPTKTTLLKMADCGGLPQISQPAKLAEAFKYFV
QGMGYMPSASMTRLMRSRTASGSSVTSLDGTRSRSHTSEGTRSRSHTSEG
TRSRSHTSEGAHLDITPNSGAAGNSAGPKSMEVSC*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU1557
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial expression with N terminal GST/FLAG tag