cDNA Clone PIPPIN

• Plasmid: pEGFP-C1-PIPPIN
• Parent Plasmid: pEGFPC1
• Expressed: GFP-PIPPIN
• Genbank: AB027011
• Tag: GFP
• Species: Human

Sequence of Insert

GGATCCGCCACCATGTACCCATACGATGTGCCAGATTACGCCACTTCAGA
GTCGACGTCACCCCCAGTTGTGCCCCCGCTCCACTCCCCCAAGTCCCCAG
TCTGGCCCACCTTCCCCTTCCACAGGGAGGGCAGCAGGGTCTGGGAGCGG
GGTGGTGTCCCACCTCGGGACCTACCCAGCCCTCTGCCCACCAAGCGGAC
CAGGACCTATTCAGCGACAGCCCGGGCCTCAGCTGGCCCCGTGTTCAAGG
GCGTCTGTAAGCAGTTCTCACGCTCACAGGGCCATGGCTTCATCACCCCC
GAGAACGGGTCCGAGGACATCTTCGTACATGTGTCTGACATCGAGGGGGA
GTACGTGCCAGTGGAGGGCGACGAGGTGACCTACAAGATGTGCCCTATCC
CTCCCAAGAACCAGAAGTTCCAGGCCGTGGAGGTGGTGCTCACTCAGCTG
GCCCCCCACACTCCCCACGAGACGTGGTCTGGCCAGGTCGTGGGCTCCTA
GGAATTC

Amino Acid Sequence

MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICT
TGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIF
FKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHN
VYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
YLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKSGLRSRAQASN
SAVDGTAGPGSTGSATMYPYDVPDYATSESTSPPVVPPLHSPKSPVWPTF
PFHREGSRVWERGGVPPRDLPSPLPTKRTRTYSATARASAGPVFKGVCKQ
FSRSQGHGFITPENGSEDIFVHVSDIEGEYVPVEGDEVTYKMCPIPPKNQ
KFQAVEVVLTQLAPHTPHETWSGQVVGS*

• Vector Type: Mammalian
• Antibiotic: Kan
• DU Number: DU1554
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N terminal GFP/HA for mammalian expression