cDNA Clone CHK1

• Synonyms: CHEK1
• Plasmid: pGEX6P-1-D290-476 CHK1
• Parent Plasmid: pGEX6P1
• Expressed: GST-D290-476 CHK1
• Genbank: BC004202
• Tag: GST
• Species: Human

Sequence of Insert

GAATTCATGGCAGTGCCCTTTGTGGAAGACTGGGACTTGGTGCAAACCCT
GGGAGAAGGTGCCTATGGAGAAGTTCAACTTGCTGTGAATAGAGTAACTG
AAGAAGCAGTCGCAGTGAAGATTGTAGATATGAAGCGTGCCGTAGACTGT
CCAGAAAATATTAAGAAAGAGATCTGTATCAATAAAATGCTAAATCATGA
AAATGTAGTAAAATTCTATGGTCACAGGAGAGAAGGCAATATCCAATATT
TATTTCTGGAGTACTGTAGTGGAGGAGAGCTTTTTGACAGAATAGAGCCA
GACATAGGCATGCCTGAACCAGATGCTCAGAGATTCTTCCATCAACTCAT
GGCAGGGGTGGTTTATCTGCATGGTATTGGAATAACTCACAGGGATATTA
AACCAGAAAATCTTCTGTTGGATGAAAGGGATAACCTCAAAATCTCAGAC
TTTGGCTTGGCAACAGTATTTCGGTATAATAATCGTGAGCGTTTGTTGAA
CAAGATGTGTGGTACTTTACCATATGTTGCTCCAGAACTTCTGAAGAGAA
GAGAATTTCATGCAGAACCAGTTGATGTTTGGTCCTGTGGAATAGTACTT
ACTGCAATGCTCGCTGGAGAATTGCCATGGGACCAACCCAGTGACAGCTG
TCAGGAGTATTCTGACTGGAAAGAAAAAAAAACATACCTCAACCCTTGGA
AAAAAATCGATTCTGCTCCTCTAGCTCTGCTGCATAAAATCTTAGTTGAG
AATCCATCAGCAAGAATTACCATTCCAGACATCAAAAAAGATAGATGGTA
CAACAAACCCCTCAAGAAAGGGGCAAAAAGGCCCCGAGTCACTTCAGGTG
GTGTGTCAGAGTCTCCCAGTGGATGAGCGGCCGCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSPEFMAVPFVEDWDLVQTLG
EGAYGEVQLAVNRVTEEAVAVKIVDMKRAVDCPENIKKEICINKMLNHEN
VVKFYGHRREGNIQYLFLEYCSGGELFDRIEPDIGMPEPDAQRFFHQLMA
GVVYLHGIGITHRDIKPENLLLDERDNLKISDFGLATVFRYNNRERLLNK
MCGTLPYVAPELLKRREFHAEPVDVWSCGIVLTAMLAGELPWDQPSDSCQ
EYSDWKEKKTYLNPWKKIDSAPLALLHKILVENPSARITIPDIKKDRWYN
KPLKKGAKRPRVTSGGVSESPSG*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU1510
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Construct for Bacterial expression of N terminal GST tag